A5047434963- Bacterial biofilms and quorum sensing
- Antimicrobial agents and applications
- Antimicrobial Peptides and Activities
- Antifungal resistance and susceptibility
- Mechanical Circulatory Support Devices
- Cardiac Structural Anomalies and Repair
- Antibiotic Resistance in Bacteria
- Reproductive tract infections research
- Advanced biosensing and bioanalysis techniques
- Fungal Infections and Studies
- Cancer-related gene regulation
- Liver Disease Diagnosis and Treatment
- RNA modifications and cancer
- Cardiac Arrest and Resuscitation
- Advanced Nanomaterials in Catalysis
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Neonatal and Maternal Infections
- Antimicrobial Resistance in Staphylococcus
- Polymer Surface Interaction Studies
- Silk-based biomaterials and applications
- Drilling and Well Engineering
- Antibiotics Pharmacokinetics and Efficacy
- Cancer Research and Treatments
- Metabolomics and Mass Spectrometry Studies
Monash University
2016-2025
The Alfred Hospital
2015-2025
Australian Regenerative Medicine Institute
2015-2025
Fudan University
2023-2025
Shaanxi Normal University
2022-2025
Yangtze University
2025
Qingdao University
2000-2025
Guangzhou University of Chinese Medicine
2022-2025
Zhejiang Chinese Medical University
2025
South China Agricultural University
2024
The fungal pathogen Candida albicans causes macrophage death and escapes, but the molecular mechanisms remained unknown. Here we used live-cell imaging to monitor interaction of C. with macrophages show that kills in two temporally mechanistically distinct phases. Early upon phagocytosis, triggers pyroptosis, a proinflammatory death. Pyroptosis is controlled by developmental yeast-to-hypha transition Candida. When pyroptosis inactivated, wild-type hyphae cause significantly less killing for...
Abstract Temozolomide (TMZ) resistance remains the major obstacle in treatment of glioblastoma (GBM). Lactylation is a novel post‐translational modification that involved various tumors. However, whether lactylation plays role GBM TMZ unclear. Here it found histone H3K9 (H3K9la) confers via LUC7L2‐mediated intron 7 retention MLH1. Mechanistically, upregulated recurrent tissues and TMZ‐resistant cells, mainly concentrated H3K9. Combined multi‐omics analysis, including CUT&Tag, SLAM‐seq,...
We have synthesized a series of copolymers containing both positively charged (amine, guanidine) and hydrophobic side chains (amphiphilic antimicrobial peptide mimics). To investigate the structure–activity relationships these polymers, low polydispersity polymethacrylates varying but uniform molecular weight composition were synthesized, using reversible addition–fragmentation chain transfer (RAFT) approach. In facile second reaction, pendant amine groups converted to guanidines, allowing...
Acinetobacter baumannii is one of the most notorious hospital-acquired pathogens, and novel treatment strategies are desperately required. Two-component regulatory systems represent potential therapeutic targets as they mediate microorganism adaptation to changing environments, often control virulence, specific bacteria. Here we describe first global virulence regulator in A. baumannii.Using transcriptional profiling functional assays a deletion mutant sensor kinase gene, A1S_0574 (termed...
Antimicrobial polymers appear as a promising alternative to tackle the current development of bacterial resistance against conventional antibiotics they rely on membrane disruption. This study investigates effect segmentation hydrophobic and cationic functionalities antimicrobial over their selectivity between bacteria mammalian cells. Using RAFT technology, statistical, diblock, highly segmented multiblock copolymers were synthesized in controlled manner. Polymers analyzed by HPLC, was...
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Sirtuins are an ancient family of NAD(+)-dependent deacylases connected with the regulation fundamental cellular processes including metabolic homeostasis and genome integrity. We show existence a hitherto unrecognized class sirtuins, found predominantly in microbial pathogens. In contrast to earlier described classes, these sirtuins exhibit robust protein ADP-ribosylation activity. our model organisms, Staphylococcus aureus Streptococcus pyogenes, activity is dependent on prior lipoylation...
Biofilm-related human infections have high mortality rates due to drug resistance. Cohabitation of diverse microbes in polymicrobial biofilms is common and these present additional challenges for treatment compared with monomicrobial biofilms. Here, we address this therapeutic gap by assessing the potential a new class antimicrobial agents, guanylated polymethacrylates, built two prominent pathogens, fungus Candida albicans bacterium Staphylococcus aureus.We used imaging quantitative methods...
We report the use of RAFT polymerization to obtain eight cationic methacrylate polymers bearing amine or guanidine pendant groups, while varying R- and Z-RAFT end-groups.
Vaginal candidiasis is an important medical condition awaiting more effective treatment. How Candida albicans causes this disease and survives antifungal treatment not yet fully understood. This study aimed to establish a comprehensive understanding of biofilm-related defensive strategies that C. uses vaginal survive treatment.A mouse model was adopted examine the formation biotic biofilms on epithelium fungal infiltration by laboratory clinical strains albicans. Histopathological changes...
Abstract Background Coagulase-negative staphylococci are major causes of bloodstream infections in very low birth weight babies cared for Neonatal Intensive Care Units. The virulence these bacteria is mainly due to their ability form biofilms on indwelling medical devices. Biofilm-related often fail respond antibiotic chemotherapy guided by conventional susceptibility tests. Methods staphylococcal blood culture isolates were grown different phases relevant biofilm formation: planktonic cells...
Coagulase-negative staphylococci (CoNS) are the main causative agents of bacteraemia in infants managed neonatal intensive care units (NICUs). Intraluminal colonization long-term central venous catheters by these bacteria and subsequent biofilm formation prerequisites bloodstream infections acquired NICUs. The catheter lock technique has been used to treat colonization; however, optimum choice antimicrobial their corresponding concentrations exposure times have not determined. effectiveness...
Objectives(i) To evaluate the role of adherent growth mode and extracellular polymer substance build-up in biofilm resistance to antibiotics. (ii) re-assess various mechanisms leading
The Mediator complex is an essential co-regulator of RNA polymerase II that conserved throughout eukaryotes. Here we present the first study in pathogenic fungus Candida albicans. We focused on Middle domain subunit Med31, Head Med20, and Srb9/Med13 from Kinase domain. C. albicans shares some roles with model yeasts Saccharomyces cerevisiae Schizosaccharomyces pombe, such as functions response to certain stresses role Med31 expression genes regulated by activator Ace2. also has additional...
Light triggered release of an antibiotic from a click crosslinked hydrogel was developed by conjugating ciprofloxacin through photo-cleavable linker to the network structure.