A5046202195- Electrospun Nanofibers in Biomedical Applications
- Nerve injury and regeneration
- 3D Printing in Biomedical Research
- Tissue Engineering and Regenerative Medicine
- Polymer Nanocomposite Synthesis and Irradiation
- Polymer Surface Interaction Studies
- Silicone and Siloxane Chemistry
- Hydrogels: synthesis, properties, applications
- Polymer Nanocomposites and Properties
- Supramolecular Self-Assembly in Materials
- Neuroscience and Neural Engineering
- Click Chemistry and Applications
- Epoxy Resin Curing Processes
- Advanced Polymer Synthesis and Characterization
- Conducting polymers and applications
- RNA Interference and Gene Delivery
- Photopolymerization techniques and applications
- Bone Tissue Engineering Materials
- Polymer crystallization and properties
- Neurogenesis and neuroplasticity mechanisms
- Advanced Sensor and Energy Harvesting Materials
- Nanofabrication and Lithography Techniques
- Pluripotent Stem Cells Research
- biodegradable polymer synthesis and properties
- Chemical Synthesis and Analysis
Monash University
2016-2025
Australian Regenerative Medicine Institute
2010-2025
Monash Institute of Medical Research
2015-2023
Australian Research Council
2023
ARC Centre of Excellence in Future Low-Energy Electronics Technologies
2021
Clayton Foundation
2014-2020
Materials Science & Engineering
2013-2018
Victoria College
2015
Cooperative Research Centre for Polymers
2001-2013
Australian National University
2011-2013
Attempts to repair the central nervous system damaged as a result of trauma or disease will depend on ability restore appropriate neuronal connectivity. This rely establishing chemical and physical environments for supporting neural cells their processes in this regard, engineering biomaterials is increasing interest. It be important understand how behave these vitro, prior future vivo application. We reveal that modification 3-dimensional (3D) electrospun poly-epsilon-caprolactone (PCL)...
Hydrogels formed by ultrashort heterochiral peptides are emerging as cost-effective materials for cell culture.
Stem cell injections for the treatment of articular cartilage damage are a promising approach to achieve tissue regeneration. However, this method is encumbered by high apoptosis rates, low retention in lesion, and inefficient chondrogenesis. Here, we have used facile, very cost-based microfluidic technique create visible light-cured microgels composed gelatin norbornene (GelNB) poly(ethylene glycol) (PEG) cross-linker. In addition, demonstrated that process enables rapid situ...
Change of chirality is a useful tool to manipulate the aqueous self-assembly behaviour uncapped, hydrophobic tripeptides. In contrast with other short peptides, these tripeptides form hydrogels at physiological pH without aid organic solvents or end-capping groups (e.g. Fmoc). The novel hydrogel forming peptide DLeu-Phe-Phe (DLFF) and its epimer Leu-Phe-Phe (LFF) exemplify dramatic supramolecular effects induced by subtle changes stereochemistry. Only D-amino acid-containing instantly forms...
Reversible photocycloaddition reactions have previously been employed in chemical cross-linking for the preparation of biomaterial scaffolds. However, processes require activation by high-energy UV light, rendering them unsuitable modification biological environments. Here we demonstrate that [2 + 2] styrylpyrene can be activated visible light at λ = 400-500 nm, enabling rapid and effective conjugation poly(ethylene glycol) (PEG) water under mild irradiation conditions (I 20 mW cm-2)....
Recent developments in photochemistry have introduced new methods to prepare hydrogels initiated by nonharmful light which is essential for encapsulation of cells and bioactive components. However, bioorthogonal photoclick reactions generally requires two components cross-linking and, many cases, the formation a reactive intermediate that may cross-react with nucleophiles biological media. Here we report utilization visible triggered dimerization electron-rich anthracene polymer form bulk...
Hydrogels are often employed as temporary platforms for cell proliferation and tissue organization in vitro. Researchers have incorporated photodegradable (PD) moieties into synthetic polymeric hydrogels a means of achieving spatiotemporal control over material properties. In this study protein‐based PD composed methacrylated gelatin crosslinker containing o ‐nitrobenzyl ester groups developed. The able to degrade rapidly specifically response UV light can be photopatterned variety shapes...
In this study, we present a method for the fabrication of in situ forming gelatin and poly(ethylene glycol)-based hydrogels utilizing bioorthogonal, strain-promoted alkyne-azide cycloaddition as cross-linking reaction. By incorporating nitrobenzyl moieties within network structure, these can be designed to degradable upon irradiation with low intensity UV light, allowing precise photopatterning. Fibroblast cells encapsulated were viable at 14 days could readily harvested using light trigger....
Electroactive materials have been investigated as next-generation neuronal tissue engineering scaffolds to enhance regeneration and functional recovery after brain injury. Graphene, an emerging scaffold material with charge transfer properties, has shown promising results for cell survival differentiation in vitro. In this vivo work, electrospun microfiber coated self-assembled colloidal graphene, were implanted into the striatum or subventricular zone of adult rats. Microglia astrocyte...
Abstract Dynamic changes in elasticity during tissue development, remodeling, and aging influence cell behavior through mechanotransduction, yet most studies rely on hydrogels with fixed mechanical properties. Although photoresponsive azobenzene‐based can control substrate stiffness dynamically, they require UV light, which damage cells DNA. This makes it difficult to determine whether cellular responses are due or UV‐induced damage. study develops a polyacrylamide‐azobenzene hydrogel system...
Neurological disorders such as traumatic brain injuries or stroke result in neuronal loss and disruption of the parenchyma. Current treatment strategies are limited that they can only mitigate degeneration process alleviate symptoms but do not reverse condition. In contrast, regenerative cell-based therapies offer long-term hope for many patients. Bioactive scaffolds likely to reinforce success cell replacement by providing a microenvironment facilitates survival, proliferation,...
Development of biomaterials with specific characteristics to influence cell behaviour has played an important role in exploiting strategies promote nerve regeneration. The effect three-dimensional (3D) non-woven electrospun poly(ε-caprolactone) (PCL) scaffolds on the rat brain-derived neural stem cells (NSCs) is reported. interaction NSCs randomly orientated submicron fibrous scaffolds, average fibre diameter 750 ± 100 nm, was investigated. PCL were modified ethylenediamine (ED) determine if...
Electroactive scaffolds that are passively conductive and able to transmit applied electrical stimuli of increasing importance for neural tissue engineering. Here, we report a process rendering both 2D 3D polymer electrically conducting, while also enhancing neuron attachment. Graphene-heparin/poly-l-lysine polyelectrolytes were assembled via layer-by-layer (LbL) deposition onto surfaces electrospun nanofibers. The employed LbL coating technique in this work enables the electro-...
We report a new class of β-peptide based hydrogel for neural tissue engineering. Our forms network nanofibres in aqueous solution, resulting stable at physiological conditions. The shows excellent compatibility with cells and provides suitable environment to adhere proliferate.
We report a new class of amyloid-inspired peptide hydrogels that was designed and based on α-synuclein protein for which hydrogel formation is triggered by various stimuli, such as heating/cooling or changes in pH. The peptides resemble cross-β-sheet-rich amyloid, they assemble into nanofibrous meshwork mimics the natural extracellular matrix. Our design principle allows easy manipulation gelator sequence to exploit desirable properties amyloids use cell replacement therapies...