A5046882263- Antimicrobial Peptides and Activities
- Immune Response and Inflammation
- Probiotics and Fermented Foods
- Reproductive tract infections research
- Parasitic Infections and Diagnostics
- Milk Quality and Mastitis in Dairy Cows
- Microbial infections and disease research
- Toxoplasma gondii Research Studies
- Amoebic Infections and Treatments
- Gut microbiota and health
- Reproductive Physiology in Livestock
- Clostridium difficile and Clostridium perfringens research
- Infant Nutrition and Health
- Biochemical and Structural Characterization
- Herpesvirus Infections and Treatments
- Ruminant Nutrition and Digestive Physiology
- Escherichia coli research studies
- Vector-Borne Animal Diseases
- Dermatology and Skin Diseases
- Autophagy in Disease and Therapy
- Animal Behavior and Welfare Studies
- Helicobacter pylori-related gastroenterology studies
- Animal Disease Management and Epidemiology
- Viral gastroenteritis research and epidemiology
- Helminth infection and control
University of Calgary
2016-2025
Département Santé Animale
2018
University of California, San Diego
2009-2012
Institut Langevin
2010
Arkema (France)
2010
University of California, Davis
2004-2007
National Agricultural Technology Institute
2003-2004
Consejo Nacional de Investigaciones Científicas y Técnicas
2001-2004
Centro Científico Tecnológico - San Juan
2004
In this study we identified mechanisms at the colonic mucosa by which MUC2 mucin regulated production of β-defensin in a proinflammatory milieu but functionally protected susceptible bacteria from its antimicrobial effects. The regulator role on 2 combination with cytokine interleukin-1β (IL-1β) was confirmed using purified human and goblet cells short hairpin RNA (shRNA) silenced for MUC2. vivo, Muc2−/− mice showed impaired mRNA expression peptide localization colon as compared Muc2+/−...
ABSTRACT Embedded in the colonic mucus are cathelicidins, small cationic peptides secreted by epithelial cells. Humans and mice have one cathelicidin-related antimicrobial peptide (CRAMP) each, LL-37/hCAP-18 Cramp, respectively, with related structure functions. Altered production of MUC2 mucin is characteristic intestinal amebiasis. The interactions between cathelicidins conferring innate immunity against Entamoeba histolytica not well characterized. In this study, we quantified whether...
Introduction Calves are highly susceptible to gastrointestinal infection with Cryptosporidium parvum ( C. ), which can result in watery diarrhea and eventually death or impaired development. With little no effective therapeutics, understanding the host’s microbiota pathogen interaction at mucosal immune system has been critical identify test novel control strategies. Methods Herein, we used an experimental model of challenge neonatal calves describe clinical signs histological proteomic...
Metronidazole and other 5-nitroimidazoles (5-NI) are among the most effective antimicrobials available against many important anaerobic pathogens, but evolving resistance is threatening their long-term clinical utility. The common 5-NIs were developed decades ago, yet little 5-NI drug development has since taken place, leaving true potential of this class unexplored. Here we report on a unique approach to modular synthesis diversified for broad exploration antimicrobial potential. Many more...
Entamoeba histolytica cysteine proteinases (EhCPs) play a key role in disrupting the colonic epithelial barrier and innate host immune response during invasion of E. histolytica, protozoan cause human amebiasis. EhCPs are encoded by 50 genes, which ehcp4 (ehcp-a4) is most up-regulated colonization mouse cecal model Up-regulation vivo correlated with our finding that co-culture trophozoites mucin-producing T84 cells increased expression up to 6-fold. We have expressed recombinant EhCP4, was...
We hypothesized that the antimicrobial peptide cathelicidin has a physiological role in regulating gut inflammatory homeostasis. determined synergizes with LPS to facilitate its internalization and signaling via endosomic TLR4 colonic epithelium, evoking synthesis of human neutrophil chemoattractant, CXCL8 (or murine homolog, CXCL1). Interaction control CXCL8/CXCL1 was assessed colon epithelial cells, colonoids cathelicidin-null mice (Camp−/-). Mechanistically, (LL-37), as an extracellular...
Streptococcus uberis mastitis in cattle infects mammary epithelial cells. Although oxidative responses often remove intracellular microbes, S. survives, but the mechanisms are not well understood. Herein, we aimed to elucidate antioxidative during pathogenesis of after isolation from clinical bovine milk samples. uberis’s vitro pathomorphology, stress biological activities, transcription factors, inflammatory response cytokines, autophagosome and autophagy functions were evaluated, vivo was...
The enteric protozoan parasite Entamoeba histolytica is the cause of potentially fatal amebic colitis and liver abscesses. E. trophozoites colonize colon, where they induce inflammation, penetrate mucosa, disrupt host immune system. early establishment in colon occurs presence antimicrobial human (LL-37) murine (CRAMP [cathelin-related peptide]) cathelicidins, essential components mammalian innate defense system intestine. Studying this step pathogenesis colitis, we demonstrate that or their...
Trichomonas vaginalis and Tritrichomonas foetus cause common sexually transmitted infections in humans cattle, respectively. Mouse models of trichomoniasis are important for pathogenic therapeutic studies. Here, we compared murine genital with T. foetus. Persistent vaginal infection was established 100 parasites but required doses 10(6), perhaps because greater susceptibility to killing by mouse polymorphonuclear leukocytes. Infection persisted longest after combined treatment mice estrogen...
Abstract Background Diarrheal diseases are a threat to human health, being the second leading cause of death in children. Diarrheic enterocolitis can be caused by attaching/effacing (A/E) pathogens like enteropathogenic and enterohemorrhagic Escherichia coli (EPEC EHEC). Citrobacter rodentium (CR), mouse A/E pathogen, mimics EPEC EHEC infections, causing goblet cell depletion, crypt hyperplasia, leukocyte infiltration. Autophagy, critical immune pathway linked mucus secretion, is involved...
Abstract Background Citrobacter rodentium (CR) is a murine attaching and effacing enteric bacterial pathogen that induces colitis mimics enteropathogenic enterohemorrhagic E. coli in humans. Studies of macrophages during CR infection have focused on proinflammatory functions, paying less attention to M2 or regulatory macrophages. We shown IL-4-treated (M(IL4)) promising potential as novel anti-colitic therapy using chemical-induced mice. However, any benefit M(IL4)s infectious unknown. This...