A5037912517- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- CAR-T cell therapy research
- Cancer Immunotherapy and Biomarkers
- Reproductive System and Pregnancy
- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Virus-based gene therapy research
- Cancer Research and Treatments
- Immune Response and Inflammation
- Viral Infections and Outbreaks Research
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Vectors
- Lung Cancer Treatments and Mutations
- RNA Interference and Gene Delivery
- Mosquito-borne diseases and control
- Respiratory viral infections research
- Cancer Genomics and Diagnostics
- Immune cells in cancer
- Antimicrobial Peptides and Activities
- Polydiacetylene-based materials and applications
- Cancer Cells and Metastasis
- Cellular Mechanics and Interactions
- SARS-CoV-2 detection and testing
Ben-Gurion University of the Negev
2016-2025
Iuliu Hațieganu University of Medicine and Pharmacy
2021
Czech Academy of Sciences, Institute of Biotechnology
2020
Faculty of Public Health
2012-2018
The Technological College of Beer Sheva
2007-2018
National Center for Biotechnology
2012
DKFZ-ZMBH Alliance
2011
German Cancer Research Center
2011
Heidelberg University
2011
Hebrew University of Jerusalem
2001-2010
Cutaneous gene (DNA) bombardment results in substantial expression of the encoded antigen epidermal layer as well detectable dendritic cells (DC) draining lymph nodes (LNs). Under these conditions, two possible modes DC presentation to naive CD8+ T might exist: (a) directly by gene-transfected trafficking local nodes, and (b) cross-presentation untransfected released from or associated with transfected cells. The relative contributions distinct priming for cytotoxic cell (CTL) responses have...
Abstract Natural killer (NK) cells destroy virus-infected and tumor without prior antigen stimulation. The NK cell cytotoxicity is regulated in large part by the expression of receptors that are able to bind major histocompatibility complex (MHC) class I glycoproteins. also express lysis triggering specific for non-MHC ligands, including NKp30, NKp44, NKp46 CD16. However, nature their recognized on target cells, undefined. We have recently shown protein, but not CD16 recognizes hemagglutinin...
Recent reports suggest that 10 to 30% of severe acute respiratory syndrome coronavirus 2 (SARS- CoV-2) infected patients are asymptomatic and viral shedding may occur before symptom onset. Therefore, there is an urgent need increase diagnostic testing capabilities prevent disease spread. We developed P-BEST, a method for Pooling-Based Efficient SARS-CoV-2 Testing, which identifies all positive subjects within set samples using single round testing. Each sample assigned into multiple pools...
It has previously been shown that bone marrow-generated dendritic cells (DC) are potent stimulators in allogeneic mixed leukocyte reactions and capable of activating naive CD4+ T situ an antigen-specific manner. In this study we have investigated whether DC inducing CD8+ cell responses vivo. Initial attempts to induce specific cytotoxic lymphocyte (CTL) mice injected with pulsed ovalbumin (OVA) peptide were frustrated by the presence high levels nonspecific lytic activity, which obscured,...
We have previously shown that bone marrow-generated dendritic cells (DC) pulsed with a class I-restricted peptide are potent inducers of CD8+ CTL. In the present study we investigated whether DC capable inducing antitumor immunity. show single immunization OVA was highly effective in eliciting protective immune response against challenge tumor expressing gene (E.G7-OVA), more so than irradiated E.G7-OVA cells, peptide-pulsed RMA-S or free mixed adjuvant. The addition protein to day 4 7...
Cellular identity and differentiation are determined by epigenetic programs. The characteristics of these programs in normal human mammary epithelium their similarity to those stem cells unknown. To begin investigating issues, we analyzed the DNA methylation gene expression profiles distinct subpopulations epithelial using MSDK (methylation-specific digital karyotyping) SAGE (serial analysis expression). We identified discrete cell-type state-specific patterns that were maintained a subset...
Abstract Natural killer (NK) cells are immune sensing and eliminating foreign, stressed, transformed, senescent through specialized surface receptors, such as NKG2D, that interacts with several virus- or stress-inducible ligands, including ULBP1 -2, which expressed on target cell surfaces. For example, induction of DNA damage cellular senescence pathways in tumor led to upregulation NKG2D ligands activate NK cells. Although, both p53, the relationship p53 activation has not been addressed....
Abstract We studied the role of NK cell-activating receptors and their ligands in lysis mononuclear phagocytes infected with intracellular pathogen Mycobacterium tuberculosis. Expression activating NKp30, NKp46, NKG2D were enhanced on cells by exposure to M. tuberculosis-infected monocytes, whereas expression DNAX accessory molecule-1 2B4 was not. Anti-NKG2D anti-NKp46 inhibited cell but Abs molecule-1, had no effect. Infection monocytes up-regulated ligand, UL-16 binding protein (ULBP)1,...
NK cells play an important role in the early immune response to cancer. The NKp44 activating receptor is only natural cytotoxicity that expressed exclusively by primate cells, yet its cellular ligands remain largely unknown. Proliferating cell nuclear Ag (PCNA) overexpressed cancer cells. In this study, we show recognizes PCNA. Their interaction inhibits function through NKp44/ITIM. physical of and PCNA enabled recruitment target immunological synapse. We demonstrate promotes survival...
Abstract We previously showed that human NK cells used the NKp46 receptor to lyse Mycobacterium tuberculosis H37Ra-infected monocytes. To identify ligands on mononuclear phagocytes, we anti-NKp46 immunoprecipitate from bound its ligand(s) Mass spectrometry analysis identified a 57-kDa molecule, vimentin, as putative ligand for NKp46. Vimentin expression was significantly up-regulated surface of infected monocytes, compared with uninfected cells, and this confirmed by fluorescence microscopy....
Natural Killer (NK) cells recognize and destroy tumors virus-infected in an antibody-independent manner. The regulation of NK is mediated by activating inhibiting receptors on the cell surface. One important family natural cytotoxicity (NCRs) which include NKp30, NKp44 NKp46. NCRs initiate tumor targeting recognition heparan sulfate cancer cells. This study aims to elucidate structural motifs that are for NCR binding. Microarray surface plasmon resonance experiments with a small library...
Dengue virus (DV) and West Nile (WNV) have become a global concern due to their widespread distribution ability cause variety of human diseases. Antiviral immune defenses involve NK cells. In the present study, we investigated interaction between cells these two flaviviruses. We show that NK-activating receptor NKp44 is involved in virally mediated activation through direct with flavivirus envelope protein. Recombinant directly binds purified DV WNV proteins specifically domain III protein;...
Abstract We used human tuberculosis as a model to investigate the role of NK cytotoxic mechanisms in immune response intracellular infection. Freshly isolated cells and cell lines from healthy donors lysed Mycobacterium tuberculosis-infected monocytes greater extent than uninfected monocytes. Lysis infected was associated with increased expression mRNA for NKp46 receptor, but not NKp44 receptor. Antisera markedly inhibited lysis cell-mediated due reduced MHC class I molecules on surface or...
Initiation of the adaptive immune response is dependent on priming naive T cells by APCs. Proteomic analysis unactivated and activated human NK cell membrane–enriched fractions demonstrated that can efficiently stimulate cells, since they upregulate MHC class II molecules multiple ligands for TCR costimulatory molecules. Furthermore, manipulating antigen administration, we show possess independent unique pathways uptake. These results highlight cell–mediated cytotoxicity specific ligand...