A5088400206- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Microbial Community Ecology and Physiology
- Enzyme Structure and Function
- Advanced biosensing and bioanalysis techniques
- Genomics and Phylogenetic Studies
- Hemoglobin structure and function
- Porphyrin Metabolism and Disorders
- Monoclonal and Polyclonal Antibodies Research
- Astronomy and Astrophysical Research
- Immune Cell Function and Interaction
- DNA and Nucleic Acid Chemistry
- Microbial metabolism and enzyme function
- Stellar, planetary, and galactic studies
- Heme Oxygenase-1 and Carbon Monoxide
- Biochemical and Structural Characterization
- Analytical Chemistry and Chromatography
- Astronomical Observations and Instrumentation
- Machine Learning in Bioinformatics
- Enzyme function and inhibition
- Neuropeptides and Animal Physiology
- Retinoids in leukemia and cellular processes
- Alcohol Consumption and Health Effects
- Click Chemistry and Applications
- Spondyloarthritis Studies and Treatments
ETH Zurich
2009-2024
University of Zurich
2009-2024
University of Vienna
2024
Genomics (United Kingdom)
2015
University of Kassel
2008-2013
Instituto de Investigaciones Químicas
2009
Ben-Gurion University of the Negev
2009
École Polytechnique Fédérale de Lausanne
2005-2007
Technische Universität Braunschweig
2002-2004
Killing Pseudomonas Gram-negative bacteria are opportunistic pathogens, and drug-resistant strains present a serious health problem. Srinivas et al. (p. 1010 ) synthesized family of peptidomimetic antibiotics that is active only against . These do not lyse the cell membrane, but instead target an essential outer membrane protein, LptD, which plays role in assembly lipopolysaccharide membrane. Activity mouse infection model suggests might have therapeutic potential. In addition, LptD widely...
Cellular senescence can exert dual effects in tumors, either suppressing or promoting tumor progression. The senescence-associated secretory phenotype (SASP), released by senescent cells, plays a crucial role this dichotomy. Consequently, the clinical challenge lies developing therapies that safely enhance cancer, favoring tumor-suppressive SASP factors over tumor-promoting ones. Here, we identify retinoic-acid-receptor (RAR) agonist adapalene as an effective pro-senescence compound prostate...
Natural Killer (NK) cells recognize and destroy tumors virus-infected in an antibody-independent manner. The regulation of NK is mediated by activating inhibiting receptors on the cell surface. One important family natural cytotoxicity (NCRs) which include NKp30, NKp44 NKp46. NCRs initiate tumor targeting recognition heparan sulfate cancer cells. This study aims to elucidate structural motifs that are for NCR binding. Microarray surface plasmon resonance experiments with a small library...
We performed a systematic comparison of three label-free methods for quantitative assessment binding strengths proteins interacting with small molecule ligands. The performance (1) nanoelectrospray ionization mass spectrometry (nESI-MS), (2) surface plasmon resonance (SPR), and (3) isothermal titration calorimetry (ITC) was compared the determination dissociation constants (K(D)). model system studied this purpose human carbonic anhydrase I (hCAI) eight known well characterized sulfonamide...
Rationale: Hemolysis occurs not only in conditions such as sickle cell disease and malaria but also during transfusion of stored blood, extracorporeal circulation, sepsis. Cell-free Hb depletes nitric oxide (NO) the vasculature, causing vasoconstriction eventually cardiovascular complications. We hypothesize that Hb-binding proteins may preserve vascular NO signaling hemolysis.Objectives: Characterization an archetypical function by which scavenger could hemolysis.Methods: investigated...
ABSTRACT The species B human adenoviruses (HAdVs) infect cells upon attaching to CD46 or desmoglein 2 (DSG-2) by one several of their 12 fiber knob trimers (FKs). To test whether DSG-2 and simultaneously serve as virus receptors for adenovirus type 3 (Ad3), we performed individual combined CD46/DSG-2 loss-of-function studies in lung A549 16HBE14o cells. Our results suggest that these cells, functions a major attachment receptor Ad3, whereas exerts minor contribution uptake the range ∼10%....
Dual control of cellular heme levels by extracellular scavenger proteins and degradation oxygenases is essential in diseases associated with increased release. During severe hemolysis or rhabdomyolysis, uncontrolled exposure can cause acute kidney injury endothelial cell damage. The toxicity was primarily attributed to its pro-oxidant effects; however additional mechanisms have not been studied systematically. In addition redox reactivity, may adversely alter functions binding impairing...
In Escherichia coli the first common precursor of all tetrapyrroles, 5-aminolevulinic acid, is synthesized from glutamyl-tRNA (Glu-tRNA(Glu)) in a two-step reaction catalyzed by reductase (GluTR) and glutamate-1-semialdehyde 2,1-aminomutase (GSA-AM). To protect highly reactive intermediate (GSA), tight complex between these two enzymes was proposed based on their solved crystal structures. The existence this hypothetical verified independent biochemical techniques. Co-immunoprecipitation...
Land plants (embryophytes) evolved in the presence of prokaryotic microbes. As a result, numerous mutually beneficial associations (symbioses) developed that can be analyzed using variety methods. Here we describe isolation and characterization new pink-pigmented facultatively methylotrophic symbiotic bacterium genus Methylobacterium (laboratory strain F3.2) was isolated from gametophytic phylloids common cord moss Funaria hygrometrica Hedw. Plantlets were collected field laboratory....
In the first step of tetrapyrrole biosynthesis in<i>Escherichia coli</i>, glutamyl-tRNA reductase (GluTR, encoded by <i>hemA</i>) catalyzes NADPH-dependent reduction to glutamate-1-semialdehyde. Soluble homodimeric <i>E. coli</i> GluTR was made co-expressing the<i>hemA</i> gene and chaperone genes <i>dnaJK</i> and<i>grpE</i>. During Mg<sup>2+</sup>-stimulated catalysis, reactive sulfhydryl group Cys-50 in enzyme attacks α-carbonyl tRNA-bound glutamate. The resulting thioester intermediate...
A pink-pigmented, facultatively methylotrophic bacterium, designated strain JT1(T), was isolated from a thallus of the liverwort Marchantia polymorpha L. and analysed by using polyphasic approach. Comparative 16S rRNA gene sequence analysis placed in clade with Methylobacterium adhaesivum AR27(T), fujisawaense DSM 5686(T), radiotolerans JCM 2831(T) jeotgali S2R03-9(T), which it showed similarities 97.8, 97.7, 97.2 97.4 %, respectively. However, levels DNA-DNA relatedness between JT1(T) these...
Background: Sprinting, a high-intensity, short-duration exercise, induces oxidative stress. This causes molecular and ultrastructural alterations. Antioxidant supplementation may mitigate side effects of near or complete exhaustion. Methods: Twenty-eight healthy male adult rats received orally normal saline, carboxymethylcellulose (vehicle), artificial, N-acetylcysteine natural antioxidant, Rutin. Rats were subjected to treadmill sprinting at increasing speeds for 5 days/week. After 26 days,...
Preclinical studies have evaluated haptoglobin (Hp) polymers from pooled human plasma as a therapeutic protein to attenuate toxic effects of cell-free hemoglobin (Hb). Proof concept demonstrated efficacy Hp in hemolysis associated with transfusion and sickle cell anemia. However, phenotype-specific products might be desirable exploit phenotype specific activities 1-1 versus 2-2, offering opportunities for recombinant therapeutics. Prohaptoglobin (proHp) is the primary translation product...
During the first step of porphyrin biosynthesis in Archaea, most bacteria, and chloroplasts glutamyl-tRNA reductase (GluTR) catalyzes NADPH-dependent reduction to glutamate-1-semialdehyde. Elements tRNAGlu important for utilization by Escherichia coli GluTR were determined kinetic analysis 51 variant transcripts E. Glu-tRNAGlu. Base U8, U13*G22**A46 base triple, tertiary Watson-Crick pair 19*56, lack residue 47 are required recognition. All these bases contribute formation unique core...
ABC transporters use the energy from binding and hydrolysis of ATP to import or extrude substrates across membrane. Using ribosome display, we raised designed ankyrin repeat proteins (DARPins) against detergent solubilized LmrCD, a heterodimeric multidrug exporter Lactococcus lactis. Several target-specific DARPin binders were identified that bind at least three distinct, partially overlapping epitopes on LmrD in solution as well native membranes. Remarkably, functional screening...
DNA modifications drive aging, neurodegeneration, carcinogenesis, and chemotherapy drug action. To understand the functional genomic roles of modifications, it is critical to accurately map their diverse chemical forms with single-nucleotide precision in complex genomes, but remains challenging. Click-code-seq a click-chemistry-aided single-nucleotide-resolution strategy for guanine-oxidation mapping, used yeast having poor applicability human genomes. Here, we upgraded click-code-seq enable...
Current methods for the determination of molecular interactions are widely used in analytical sciences. To identify new methods, we investigated as a model system hybridisation short 7 nt oligonucleotide labelled with, structurally, very similar cyanine dyes CY3 and DY-547, respectively, to 34 probe immobilised zero-mode waveguide (ZMW) nanostructure. Using modified commercial off-the-shelf DNA sequencer, established principles measure biomolecular at single-molecule level. Kinetic data were...
The initial step of tetrapyrrole biosynthesis in Escherichia coli involves the NADPH‐dependent reduction by glutamyl‐tRNA reductase (GluTR) tRNA‐bound glutamate to glutamate‐1‐semialdehyde. We evaluated contribution moiety substrate specificity vitro using a range substrates and enzyme variants. Unexpectedly, we found that tRNA Glu mischarged with glutamine was for purified recombinant GluTR. Similarly unexpectedly, substitution amino acid residues involved side chain binding (S109A, T49V,...
Objectives HLA-B27 is a common genetic risk factor for the development of Spondyloarthritides (SpA). can misfold to form cell-surface heavy chain homodimers (B272) and induce pro-inflammatory responses that may lead SpA pathogenesis. The presence B272 be detected on leukocytes HLA-B27+ Ankylosing spondylitis (AS) patients transgenic rats. We characterized novel B272–specific monoclonal antibody study its therapeutic use in associated disorders. Methods HD5 was selected from phage library...
The analysis of non-variable stars is generally neglected in the literature. However, such objects are needed for many calibration processes and testing pulsational models. photometric time series Kepler satellite mission still stand as most accurate data available today excellently suited to search stars. We analysed all long-cadence light curves not reported a variable so far from mission. Using known characteristics flaws these sets, we defined three different frequency ranges where...
On the surface of healthy land plants (embryophytes), numerous non-pathogenic bacteria have been discovered and described. Among these epiphytic microbes, pink-pigmented facultative methylotrophic microbes genus Methylobacterium are special significance, because microorganisms consume methanol emitted via stomatal pores secrete growth-promoting phytohormones. funariae, Schauer Kutschera 2011, a species isolated in our lab from common cord moss, described as nova this journal, was recently...