A5056886474- Virus-based gene therapy research
- Viral Infectious Diseases and Gene Expression in Insects
- CAR-T cell therapy research
- RNA Interference and Gene Delivery
- Viral gastroenteritis research and epidemiology
- Bacteriophages and microbial interactions
- Herpesvirus Infections and Treatments
- interferon and immune responses
- Viral Infections and Immunology Research
- Cellular transport and secretion
- Cell Image Analysis Techniques
- RNA Research and Splicing
- Cytomegalovirus and herpesvirus research
- Animal Virus Infections Studies
- Nuclear Structure and Function
- CRISPR and Genetic Engineering
- Advanced Fluorescence Microscopy Techniques
- RNA and protein synthesis mechanisms
- Microtubule and mitosis dynamics
- SARS-CoV-2 and COVID-19 Research
- Respiratory viral infections research
- Diabetic Foot Ulcer Assessment and Management
- Digital Imaging for Blood Diseases
- Endoplasmic Reticulum Stress and Disease
- Plant Virus Research Studies
University of Zurich
2016-2025
Heidelberg University
2016
Scripps Research Institute
1992-2002
Cancer Research Institute
2002
Johns Hopkins University
2002
Emory University
2002
Yale University
1993-1996
Scripps Clinic
1990
ETH Zurich
1988-1989
École Polytechnique Fédérale de Lausanne
1986
Another host factor for SARS-CoV-2 Virus-host interactions determine cellular entry and spreading in tissues. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) the earlier SARS-CoV use angiotensin-converting enzyme (ACE2) as a receptor; however, their tissue tropism differs, raising possibility that additional factors are involved. The spike protein of contains cleavage site protease furin is absent from (see Perspective by Kielian). Cantuti-Castelvetri et al. now show...
Adenovirus type 2 (Ad2) binds the coxsackie B virus Ad receptor and is endocytosed upon activation of αv integrin coreceptors. Here, we demonstrate that expression dominant negative clathrin hub, eps15, or K44A-dynamin (dyn) inhibited Ad2 uptake into epithelial cells, indicating clathrin-dependent viral endocytosis. Surprisingly, strongly stimulated endocytic fluid phase tracers, coincident with internalization but without affecting receptor-mediated transferrin uptake. A large amount...
Adenovirus (Ad) enters target cells by receptor-mediated endocytosis, escapes to the cytosol, and then delivers its DNA genome into nucleus. Here we analyzed trafficking of fluorophore-tagged viruses in HeLa TC7 time-lapse microscopy. Our results show that native or taxol-stabilized microtubules (MTs) support alternating minus- plus end–directed movements cytosolic virus with elementary speeds up 2.6 μm/s. No directed movement was observed nocodazole-treated cells. Switching between plus-...
Viruses use a limited set of host pathways for infection. These represent bona fide antiviral targets with low likelihood viral resistance. We identified the salicylanilide niclosamide as broad range agent targeting acidified endosomes. Niclosamide is approved human against helminthic infections, and has anti-neoplastic effects. Its mode action unknown. Here, we show that niclosamide, which weak lipophilic acid inhibited infection pH-dependent rhinoviruses (HRV) influenza virus....
ABSTRACT Many human adenovirus (Ad) serotypes use the coxsackie B virus-Ad receptor (CAR). Recently, CD46 was suggested to be a of species Ad serotype 11 (Ad11), Ad14, Ad16, Ad21, Ad35, and Ad50. Using Sindbis virus-mediated cDNA library expression, we identify here membrane cofactor protein as surface Ad3. All four major transcripts one minor transcript expressed in nucleated cells were isolated. Rodent BHK stably expressing BC1 form bound radiolabeled Ad3 with dissociation constant 0.3 nM,...
The tumor suppressor protein p53 localizes to microtubules (MT) and, in response DNA damage, is transported the nucleus via MT minus-end-directed motor dynein. Dynein also responsible for MT-mediated nuclear targeting of adenovirus type 2 (Ad2). Here we show that treatment with low concentrations MT-targeting compounds (MTCs) do not disrupt network but are known suppress dynamics enhanced accumulation, and activation p53-downstream target genes. accumulation required binding MTCs MTs...
Nuclear pore complexes provide channels for molecular transport across the nuclear envelope. Translocation of most proteins and RNAs through complex is mediated by signal- ATP-dependent mechanisms, while small molecules accomplished passive diffusion. We report here that depletion calcium from lumen endoplasmic reticulum envelope with ionophores or pump inhibitor thapsigargin rapidly potently inhibits signal into nucleus. Lumenal also diffusion complex. Signal-mediated protein import are...
Isogenic cells in culture show strong variability, which arises from dynamic adaptations to the microenvironment of individual cells. Here we study influence cell population context, determines a single cell's microenvironment, image-based RNAi screens. We developed comprehensive computational approach that employs Bayesian and multivariate methods at single-cell level. applied these 45 RNA interference screens various sizes, including 7 druggable genome 2 genome-wide screens, analysing 17...