A5033453546- Welding Techniques and Residual Stresses
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Genetic factors in colorectal cancer
- Advanced Welding Techniques Analysis
- Advanced Fluorescence Microscopy Techniques
- Hydrogen embrittlement and corrosion behaviors in metals
- Microstructure and Mechanical Properties of Steels
- Cultural and Historical Studies
- Advanced biosensing and bioanalysis techniques
- DNA Repair Mechanisms
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- Engineering Applied Research
- Bacterial Genetics and Biotechnology
- Fatigue and fracture mechanics
- Laser and Thermal Forming Techniques
- Consumer Perception and Purchasing Behavior
- Force Microscopy Techniques and Applications
- DNA and Nucleic Acid Chemistry
- Metal Forming Simulation Techniques
- Metallurgy and Material Forming
- Advanced Biosensing Techniques and Applications
- Liquid Crystal Research Advancements
- Aluminum Alloy Microstructure Properties
Pohang University of Science and Technology
2016-2025
Korea Post
2024
Asia Pacific Center for Theoretical Physics
2022
Chosun University
2018
Agency for Defense Development
2010
Pohang Iron and Steel (South Korea)
2003-2009
Brandeis University
2001-2007
Harvard University
2006-2007
Sun Moon University
2006
Pusan National University
2004
Membrane nanotubes or tunneling (TNTs) that connect cells have been recognized as a previously unidentified pathway for intercellular transport between distant cells. However, it is unknown how this delicate structure, which extends over tens of micrometers and remains robust hours, formed. Here, we found TNT develops from double filopodial bridge (DFB) created by the physical contact two filopodia through helical deformation DFB. The transition DFB to close-ended most likely triggered...
Temperature dependent photoluminescence (PL) spectroscopy in a range of 5 K to room temperature (RT, 290 K) and single dot blinking behavior were investigated for CdTe/CdSe (core/shell, C/S) quantum dots (QDs). The QDs show type-II characteristics as both the valence conduction band levels CdTe core are placed higher energy than those CdSe shell. thickness shell was varied control degree character, bare used controls. (C/S) have unique PL properties including (i) high susceptibility thermal...
Abstract Helicase is a nucleic acid motor that catalyses the unwinding of double-stranded (ds) RNA and DNA via ATP hydrolysis. Helicases can act either as unwinds its ds substrates or chaperone alters stability substrates, but two activities have not yet been reported to simultaneously. Here, we used single-molecule techniques unravel synergistic coordination helicase activities, found severe acute respiratory syndrome coronavirus (nsp13) capable modes action: (i) binding nsp13 in tandem...
Significance The ensemble interactions between the human mismatch repair (MMR) components during mismatch-dependent DNA excision remain poorly characterized. We have detailed these in real time to reveal new dynamic collaborations four evolutionarily conserved that ultimately result a complete MMR event. Our observations demonstrate stochastic nature of an essential genome maintenance system results robust events.
Retroviruses must integrate their linear viral cDNA into the host genome for a productive infection. Integration is catalysed by retrovirus-encoded integrase (IN), which forms tetramer or octamer complex with long terminal repeat (LTR) ends termed an intasome. IN removes two 3'-nucleotides from both LTR and catalyses strand transfer of recessed 3'-hydroxyls target DNA separated 4-6 bp. Host repair restores resulting 5'-Flap single-stranded (ssDNA) gap. Here we have used multiple single...
A shared paradigm of mismatch repair (MMR) across biology depicts extensive exonuclease-driven strand-specific excision that begins at a distant single-stranded DNA (ssDNA) break and proceeds back past the mismatched nucleotides. Historical reconstitution studies concluded Escherichia coli (Ec) MMR employed EcMutS, EcMutL, EcMutH, EcUvrD, EcSSB one four ssDNA exonucleases to accomplish excision. Recent single-molecule images demonstrated EcMutS EcMutL formed cascading sliding clamps on...
Nonsense-mediated mRNA decay (NMD) typifies an surveillance pathway. Because NMD necessitates a translation event to recognize premature termination codon on mRNAs, truncated misfolded polypeptides (NMD-polypeptides) could potentially be generated from substrates as byproducts. Here, we show that when the ubiquitin-proteasome system is overwhelmed, various including NMD-polypeptides accumulate in aggresome: perinuclear nonmembranous compartment eventually cleared by autophagy....
Basic characteristics of CO2 laser welded 800 MPa class TRIP steel such as defects, microstructure, mechanical properties and formability was investigated. Bead-on-plate welding carried out under various power, speed shield gas. Porosity fraction reduced with increasing the using Ar-He mixed gas compared to Ar The maximum hardness obtained at weld metal well HAZ near metal. value same regardless nearly equal that water quenched raw In perpendicular tensile test axis, joints produced optimum...
Curved textures of ferroelectric smectic-${C}^{*}$ liquid crystals produce space charge when they involve divergence the spontaneous polarization field. Impurity ions can partially screen this charge, reducing long-range interactions to local ones. Through studies islands on very thin free-standing smectic films, we see evidence effect, in which materials with a large have static structures described by effective bend elastic constant. To address issue, calculated electrostatic free energy...
DNA binding by MutL homologs (MLH/PMS) during mismatch repair (MMR) has been considered based on biochemical and genetic studies. Bulk studies with its yeast Mlh1-Pms1 have suggested an integral role for a single-stranded (ssDNA) activity MMR. We developed single-molecule Förster resonance energy transfer (smFRET) flow-extension assays to examine interaction ssDNA in real time. The smFRET assay allowed us observe MutL-ssDNA association dissociation. determined that required ATP was the...
Photoswitching or modulation of quantum dots (QDs) can be promising for many fields that include display, memory, and super-resolution imaging. However, such modulations have mostly relied on photomodulations conjugated molecules in QD vicinity, which typically require high power energy photons at UV. We report a visible light-induced facile route QD-dye conjugates. crystal violets conjugates (QD-CVs) were prepared the violet (CV) quenched fluorescence efficiently. The QD-CVs showed single...
We present a single-molecule diffusional-mobility-shift assay (smDIMSA) for analyzing the interactions between membrane and water-soluble proteins in crowded of living cells. found that ligand-receptor decreased diffusional mobility ErbB receptors β-adrenergic receptors, as determined by single-particle tracking with super-resolution microscopy. The shift was sensitive to size binders ranged from few tens kilodaltons several hundred kilodaltons. This technique used quantitatively analyze...
Abstract Fluorophore labeling of proteins while preserving native functions is essential for bulk Förster resonance energy transfer (FRET) interaction and single molecule imaging analysis. Here we describe a versatile, efficient, specific, irreversible, gentle low-cost method with fluorophores that appears substantially more robust than similar but chemically distinct procedure. The employs the controlled enzymatic conversion central Cys to reactive formylglycine (fGly) aldehyde within six...