A5102993006- T-cell and B-cell Immunology
- Dermatology and Skin Diseases
- RNA Research and Splicing
- Pain Mechanisms and Treatments
- CAR-T cell therapy research
- IL-33, ST2, and ILC Pathways
- Single-cell and spatial transcriptomics
- EU Law and Policy Analysis
- Genetic factors in colorectal cancer
- Cancer Immunotherapy and Biomarkers
- Merger and Competition Analysis
- Monoclonal and Polyclonal Antibodies Research
- Neuropeptides and Animal Physiology
- Immune Cell Function and Interaction
- European and International Law Studies
- RNA regulation and disease
- Gene Regulatory Network Analysis
- Gene expression and cancer classification
- Cardiomyopathy and Myosin Studies
- Lepidoptera: Biology and Taxonomy
- Genomics and Chromatin Dynamics
- Regulation of Appetite and Obesity
- Atherosclerosis and Cardiovascular Diseases
- Healthcare cost, quality, practices
- Adipose Tissue and Metabolism
University of Rochester Medical Center
2025
Campbell University
2023
Regional Medical Center
2023
University of California, San Francisco
2020-2022
San Francisco VA Medical Center
2022
Imperial College London
2020
San Francisco VA Health Care System
2020
University of Manchester
2017
University of California, Irvine
2014-2016
Vanderbilt Health
2016
Inflammatory conditions represent the largest class of chronic skin disease, but molecular dysregulation underlying many individual cases remains unclear. Single-cell RNA sequencing (scRNA-seq) has increased precision in dissecting complex mixture immune and stromal cell perturbations inflammatory disease states. We single-cell–profiled CD45 + transcriptomes from samples 31 patients (7 atopic dermatitis, 8 psoriasis vulgaris, 2 lichen planus (LP), 1 bullous pemphigoid (BP), 6...
Chronic pain attenuates midbrain dopamine (DA) transmission, as evidenced by a decrease in opioid-evoked DA release the ventral striatum, suggesting that occurrence of chronic impairs reward-related behaviors. However, mechanisms which modifies transmission remain elusive. Using vivo microdialysis and microinjection drugs into mesolimbic system, we demonstrate mice rats microglial activation VTA compromises not only DA, but also other DA-stimulating drugs, such cocaine. Our data show loss...
Abstract This study had two aims. First, we tested the monophyly of and relationships within ‘bombycoid complex’, an assembly approximately 5300 species postulated by Minet to represent 12 families in three superfamilies, sequencing five protein‐coding nuclear gene regions (CAD, DDC, enolase, period, wingless; 6750 bp total) 66 representatives most subfamilies tribes. Second, sought initial evidence on utility these genes for estimating among Macrolepidoptera more broadly (11 superfamilies...
Abstract The Saturniidae, or wild silkmoths, number approximately 1861 species in 162 genera and nine subfamilies including Cercophaninae Oxyteninae. They include some of the largest most spectacular all Lepidoptera, such as moon luna moths, atlas emperor many others. Saturniids have been important sources silk and/or human food a cultures, models for comparative studies genetics, development, physiology, ecology. Seeking to improve phylogenetic framework studies, we estimated relationships...
Inflammatory response heterogeneity has impeded high-resolution dissection of diverse immune cell populations during activation. We characterize mouse cutaneous cells by single-cell RNA sequencing, after inducing inflammation using imiquimod and oxazolone dermatitis models. identify 13 CD45+ subpopulations, which broadly represent most functionally characterized types. Oxazolone pervasively upregulates Jak2/Stat3 expression across T antigen-presenting (APCs). also induces Il4/Il13 in newly...
Abstract Introduction: Untreated avascular necrosis (AVN) can lead to femoral head collapse, which, when symptomatic, requires total hip arthroplasty (THA). Osteochondral allograft transplantation (OATS) of the was designed restore subchondral bone and articular cartilage in post-collapse AVN, ideally preventing or delaying THA. Our cohort includes patients with dysplasia who underwent simultaneous periacetabular osteotomy (PAO) OATS, outcomes which have not previously been described....
Significance Recombination between divergent DNA sequences (homeologous recombination) is generally suppressed to preserve cellular genetic integrity and ultimately introduce barriers species. Decades of cell biology studies have identified the involvement mismatch repair (MMR) machinery in quality control homologous recombination. However, molecular mechanism by which this remarkable achieved unknown. Here, we report biophysical reconstitution analysis early steps rejection MMR during...
The homeostatic mechanisms that fail to restrain chronic tissue inflammation in diseases, such as psoriasis vulgaris, remain incompletely understood. We profiled transcriptomes and epitopes of single psoriatic normal skin-resident T cells, revealing a gradated transcriptional program coordinately regulated inflammation-suppressive genes. This program, which is sharply suppressed lesional skin, strikingly restricts Th17/Tc17 cytokine other inflammatory mediators on the single-cell level....
Identifying genetic variation underlying human diseases establishes targets for therapeutic development and helps tailor treatments to individual patients. Large-scale transcriptomic profiling has extended the study of such molecular heterogeneity between patients somatic tissues. However, lower resolution bulk RNA profiling, especially in a complex, composite tissue as skin, limited its success. Here we demonstrate approaches interrogate patient-level variance chronic skin inflammatory...
Feelings of hunger carry a negative-valence (emotion) signal that appears to be conveyed through agouti-related peptide (AgRP) neurons in the hypothalamic arcuate nucleus. The circulating hormone, ghrelin, activates these although it remains unclear whether also carries signal. Given ghrelin pathways midbrain are important for reward, possible could act as positive reinforcer and hence, positive-valence Here we used condition preference/avoidance tests explore reinforcing/aversive properties...
Background Abundant, abnormal myocardial trabeculae define left ventricular noncompaction (LVNC) but measurement is difficult and at least 5 techniques are described. We hypothesized that part of the reason for difficulties was LV were fractal in nature beyond simple geometry 1 or 2 dimensional (2D) measurement. designed validated a new, rapid, clinically applicable method measuring based on analysis. Methods developed analysis technique trabeculation using CMR volume stack images. With no...
The kappa opioid receptor (KOR) is crucial for the regulation of mood and reward pathways in brain. Activation this with endogenous ligand dynorphin or KOR agonists can lead to dysphoria humans. It hypothesized that chronic neuropathic pain leads a decreased dopaminergic tone within mesocorticolimbic pathway, which could induce depression, insomnia, anxiety, demotivation, anhedonia. In study, we aimed determine role signaling on negative affective component pain. We produced (NP) adult...
Abstract A retrospective study using light microscopy was performed to assess the prevalence of surface and follicular bacteria fungi in skin biopsy specimens from 247 horses with inflammatory dermatoses 27 healthy skin. Cocci were found on 23% (95% confidence interval 18%, 29%) 7% interval, 0%, 19%), respectively, disease Of nine at least 10 cases our series horses, bacterial folliculitis had a higher (57%; 95% 34%, 81%) than other eight (which all < 30%). There significant association...