A5085805590- Alzheimer's disease research and treatments
- Amyotrophic Lateral Sclerosis Research
- SARS-CoV-2 and COVID-19 Research
- Parkinson's Disease Mechanisms and Treatments
- COVID-19 Clinical Research Studies
- Antibiotic Resistance in Bacteria
- Neurological diseases and metabolism
- Occupational and environmental lung diseases
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Prion Diseases and Protein Misfolding
- Neurogenetic and Muscular Disorders Research
- Parasite Biology and Host Interactions
- Parasitic Diseases Research and Treatment
- Dementia and Cognitive Impairment Research
- Bacterial biofilms and quorum sensing
- Long-Term Effects of COVID-19
- Antimicrobial Resistance in Staphylococcus
- Nosocomial Infections in ICU
- Gut microbiota and health
- Cholinesterase and Neurodegenerative Diseases
- Medical Imaging and Pathology Studies
- COVID-19 and healthcare impacts
- Neuroinflammation and Neurodegeneration Mechanisms
- Ubiquitin and proteasome pathways
- Computational Drug Discovery Methods
University of Antwerp
2016-2025
University of Medicine and Pharmacy of Craiova
2020
Central Pollution Control Board
2014
VIB-UAntwerp Center for Molecular Neurology
2006-2013
Virginia Commonwealth University
2012
Vlaams Instituut voor Biotechnologie
2000-2009
Mayo Clinic in Florida
2007
Hertie Institute for Clinical Brain Research
2007
German Center for Neurodegenerative Diseases
2006
University of Manchester
2006
Nomenclature and nosology for neuropathologic subtypes of frontotemporal lobar degeneration : an update
We identified a novel plasmid-mediated colistin-resistance gene in porcine and bovine colistin-resistant Escherichia coli that did not contain mcr-1. The gene, termed mcr-2, 1,617 bp phosphoethanolamine transferase harboured on an IncX4 plasmid, has 76.7% nucleotide identity to Prevalence of mcr-2 E. (11/53) Belgium was higher than mcr-1 (7/53). These data call for immediate introduction screening ongoing molecular epidemiological surveillance Gram-negative pathogens.
Neuronal cytoplasmic and intranuclear aggregates of RNA-binding protein TDP-43 are a hallmark feature neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) frontotemporal lobar degeneration (FTLD). ALS FTLD show considerable clinical pathological overlap occur both familial sporadic forms. Though missense mutations in cause rare forms ALS, it is not yet known whether this due to loss function or gain aberrant function. Moreover, the role wild-type (WT) TDP-43, associated...
Lack of specific definitions clinical characteristics, disease severity, and risk preventive factors post-COVID-19 syndrome (PCS) severely impacts research discovery new therapeutics drugs.This prospective multicenter cohort study was conducted from February 2020 to June 2022 in 5 countries, enrolling SARS-CoV-2 out- in-patients followed at 3-, 6-, 12-month diagnosis, with assessment biochemical features, antibody (Ab) response, Variant Concern (VoC), physical mental quality life (QoL)....
The varied ways in which mutations presenilins (PSEN1 and PSEN2) affect amyloid b precursor protein (APP) processing causing early-onset familial Alzheimer disease (FAD) are complex not yet properly understood. Nonetheless, one useful diagnostic marker is an increased ratio of Ab42 to Ab40 (Ab42/Ab40) patients' brain biological fluids as well transgenic mice cells. We studied Ab APP for a set nine clinical PSEN on novel highly reproducible enzyme-linked immunosorbent assay (ELISA)-based...
Through an international consortium, we have collected 37 tau- and TAR DNA-binding protein 43 (TDP-43)-negative frontotemporal lobar degeneration (FTLD) cases, present here the first comprehensive analysis of these cases in terms neuropathology, genetics, demographics clinical data. 92% (34/37) had fused sarcoma (FUS) pathology, indicating that FTLD-FUS is important FTLD subtype. This collection specifically focussed on aFTLD-U one three recently defined subtypes FTLD-FUS. The subtype...
Abstract Familial forms of frontotemporal dementia (FTD) with tauopathy are mostly caused by mutations in the gene encoding microtubule‐associated protein tau (MAPT). However, rare familial without MAPT have been reported, suggesting other tauopathy‐related genetic defects. Interestingly, two presenilin 1 (PS1) (Leu113Pro and insArg352) recently associated FTD albeit neuropathological confirmation. We report here a novel PS1 mutation patient Pick‐type absence extracellular β‐amyloid...
<h3>Background</h3> Progranulin gene (<i>PGRN</i>) haploinsufficiency was recently associated with ubiquitin-positive frontotemporal lobar degeneration linked to chromosome 17q21 (FTLDU-17). <h3>Objective</h3> To assess whether<i>PGRN</i>genetic variability contributed other common neurodegenerative brain diseases, such as Alzheimer disease (AD) or Parkinson (PD). <h3>Design</h3> Mutation analysis of<i>PGRN</i> <h3>Setting</h3> Memory Clinic of the Middelheim General Hospital....
Double or multiple antigen labeling in IHC classically relies on the existence of primary antibodies raised different species IgG isotypes to ensure specific with secondary detection systems. However, suitable pairs are not always available best choice (e.g., as diagnostic tools). During last few years, several methods have been proposed overcome this, but none them offers flexibility needed for reliable double enzymatic fluorescent IHC. We present here a procedure that elutes after first...
Abstract Loss‐of‐function mutations in progranulin ( GRN ) are associated with frontotemporal lobar degeneration intraneuronal ubiquitinated protein accumulations composed primarily of hyperphosphorylated TDP‐43 (FTLD‐TDP). The mechanism by which deficiency causes pathology or neurodegeneration remains elusive. To explore the role vivo , we established Grn knockout mice using a targeted genomic recombination approach and Cre‐LoxP technology. Constitutive homozygous (Grn −/− were born an...
The initial report of the mcr-1 (mobile colistin resistance) gene has led to many reports variants and other mcr genes from different bacterial species originating human, animal environmental samples in geographical locations. Resistance nomenclature is complex unfortunately problems such as names being used for same gene/protein or name genes/proteins are not uncommon. Registries exist some families, bla (β-lactamase) genes, but there yet no agreed scheme genes. National Center...
Abstract It is well established that antibiotic treatment selects for resistance, but the dynamics of this process during infections are poorly understood. Here we map responses Pseudomonas aeruginosa to in high definition a lung infection single ICU patient. Host immunity and therapy with meropenem suppressed P. , second wave emerged due growth oprD wbpM resistant mutants evolved situ. Selection then led loss resistance by decreasing prevalence low fitness mutants, increasing frequency...
Abstract Bacteria have the potential to translocate between sites in human body, but dynamics and consequences of within-host bacterial migration remain poorly understood. Here we investigate link gut lung Pseudomonas aeruginosa populations an intensively sampled ICU patient using a combination genomics, isolate phenotyping, host immunity profiling, clinical data. Crucially, show that colonization was driven by translocation P. from gut. Meropenem treatment for suspected urinary tract...
Abstract Objectives To determine the susceptibility profiles and resistome of Pseudomonas aeruginosa isolates from European ICUs during a prospective cohort study (ASPIRE-ICU). Methods 723 respiratory samples or perianal swabs 402 patients 29 sites in 11 countries were studied. MICs 12 antibiotics determined by broth microdilution. Horizontally acquired β-lactamases analysed through phenotypic genetic assays. The first 105 providing such WGS, including analysis previously defined genotypic...
BackgroundThe role of host immunity in emergence evasive SARS-CoV-2 Spike mutations under therapeutic monoclonal antibody (mAb) pressure remains to be explored.MethodsIn a prospective, observational, monocentric ORCHESTRA cohort study, conducted between March 2021 and November 2022, mild-to-moderately ill COVID-19 patients (n = 204) receiving bamlanivimab, bamlanivimab/etesevimab, casirivimab/imdevimab, or sotrovimab were longitudinally studied over 28 days for viral loads, de novo...
Vascular endothelial growth factor (VEGF), acidic and basic fibroblast factors (FGF-1 -2), transforming β (TGFβ) are potent angiogenic cytokines. Malignant mesothelioma of the pleura presents with a high intra-tumoural microvascular density (IMD) which also has prognostic relevance. This study was designed to verify immunohistochemical expression cytokines in as well non-neoplastic human mesothelial cells individual combined these relation both IMD prognosis. In addition, four cell lines...
Amyloidogenic processing of the amyloid precursor protein (APP) with deposition in brain 42 amino acid long beta-peptide (A beta(42)) is considered central to Alzheimer's disease (AD) pathology. However, it generally believed that nonfibrillar pre-amyloid A beta(42) deposits have mature presence beta(40) into fibrillar plaques cause neurodegeneration. Here, we describe an aggressive form AD caused by a novel missense mutation APP (T714I) directly involving gamma-secretase cleavages APP. The...