A5056220165- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Xenotransplantation and immune response
- Reproductive Biology and Fertility
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Renal and related cancers
- Nanoparticles: synthesis and applications
- Ion channel regulation and function
- Sperm and Testicular Function
- Tissue Engineering and Regenerative Medicine
- Laser-Ablation Synthesis of Nanoparticles
- Microbial Inactivation Methods
- Viral Infectious Diseases and Gene Expression in Insects
- Epigenetics and DNA Methylation
- Ovarian function and disorders
- Telomeres, Telomerase, and Senescence
- Prenatal Screening and Diagnostics
- Retinal Development and Disorders
- Gold and Silver Nanoparticles Synthesis and Applications
- Neuroscience and Neuropharmacology Research
- Microplastics and Plastic Pollution
- Biomedical Ethics and Regulation
- Organ Transplantation Techniques and Outcomes
Friedrich-Loeffler-Institut
2016-2025
Heidelberg Institute for Stem Cell Technology and Experimental Medicine
2025
Animal Health Institute
2024
Islamic Azad University, Isfahan
2021
Schön Klinik Neustadt
2014-2017
University of Duisburg-Essen
2012
Research Institute for Farm Animal Biology (FBN)
2008
Biotechnology Institute
2006
Medizinische Hochschule Hannover
2004
Faktor 10 – Institut für nachhaltiges Wirtschaften gemeinnützige
2000-2001
The success of somatic nuclear transfer critically depends on the cell cycle stage donor nucleus and recipient cytoplast. In this study we tested serum deprivation as well two reversible inhibitors, aphidicolin butyrolactone I, for their ability to synchronize porcine fetal fibroblasts at either G0 or G1/S G2/M transition. synchronization efficiency various protocols was determined by fluorescence-activated sorting (FACS), proliferation assays, semiquantitative multiplex reverse...
Protein phosphatase 2A (PP2A) is a multimeric enzyme, containing catalytic subunit complexed with two regulatory subunits. The PP2A C encoded by distinct and unlinked genes, termed Cα Cβ. specific function of these subunits unknown. To address the possible redundancy between related phosphatases as well Cβ, gene was deleted homologous recombination. Homozygous null mutant mice are embryonically lethal, demonstrating that an essential gene. As exerts range cellular functions including cell...
The enzyme telomerase is active in germ cells and early embryonic development crucial for the maintenance of telomere length. Whereas different length telomeres somatic well documented, information on regulation during embryogenesis lacking. In this study, we demonstrate a elongation program at transition from morula to blastocyst mice cattle that establishes specific set point embryogenesis. We show process restores cloned embryos derived fibroblasts, regardless donor nuclei, stage...
Metal and alloy nanoparticles are increasingly developed for biomedical applications, while a firm understanding of their biocompatibility is still missing. Various properties have been reported to influence the toxic potential nanoparticles. This study aimed assess impact nanoparticle size, surface ligands chemical composition gold, silver or gold-silver on mammalian gametes. An in vitro assay porcine gametes was developed, since these delicate primary cells, which well-established culture...
Bovine embryos can be generated by in vitro fertilization or somatic nuclear transfer; however, these differ from their vivo counterparts many aspects and exhibit a higher proportion of developmental abnormalities. Here, we determined for the first time transcriptomes bovine metaphase II oocytes all stages preimplantation developing up to blastocyst using Affymetrix GeneChip Genome Array which examines approximately 23,000 transcripts. The data show that transcribed significantly number...
Abstract: Background: Xenotransplantation using porcine cells, tissues or organs may be associated with the transmission of endogenous retroviruses (PERVs). More than 50 viral copies have been identified in pig genome and three different subtypes PERV were released from two them able to infect human cells vitro. RNA interference is a promising option inhibit transmission. Methods: We recently selected an efficient si (small interfering) corresponding highly conserved region DNA, which...
Genetic engineering can expand the utility of pigs for modeling human diseases, and developing advanced therapeutic approaches. However, inefficient production transgenic represents a technological bottleneck. Here, we assessed hyperactive Sleeping Beauty (SB100X) transposon system enzyme-catalyzed transgene integration into embryonic porcine genome. The components vector were microinjected as circular plasmids cytoplasm zygotes, resulting in high frequencies fetuses piglets. animals showed...
Gold nanoparticles (GNPs) have interesting optical properties, such as exceptionally high quantum yields and virtually limitless photostability. Therefore, they show the potential for applications biomarkers especially suitable in vivo long-term studies. The generation of GNPs using pulsed laser light rather than chemical means provides nanoparticles, which are remarkably stable a variety media without need stabilizing agents or ligands. This stabilization is achieved by partial oxidation...
The domestic pig is an important large animal model for preclinical testing of novel cell therapies. Recently, we produced pluripotency reporter pigs in which the Oct4 promoter drives expression enhanced green fluorescent protein (EGFP). Here, reprogrammed Oct4-EGFP fibroblasts employing nonviral Sleeping Beauty transposon system to deliver reprogramming factors Oct4, Sox2, Klf4, and cMyc. Successful a pluripotent state was indicated by changes morphology reactivation reporter....
The use of engineered nanoparticles has risen exponentially over the last decade. Applications are manifold and include utilisation in industrial goods as well medical consumer products. Gold silver play an important role current increase nanoparticle usage. However, our understanding concerning possible side effects this increased exposure to particles, which frequently same size regime medium sized biomolecules accessorily possess highly active surfaces, is still incomplete. That...
Induced pluripotent stem cells (iPSCs) are a seminal breakthrough in cell research and promising tools for advanced regenerative therapies humans reproductive biotechnology farm animals. iPSCs particularly valuable species which authentic embryonic (ESC) lines yet not available. Here, we describe nonviral method the derivation of bovine employing Sleeping Beauty (SB) piggyBac (PB) transposon systems encoding different combinations reprogramming factors, each separated by self-cleaving...
Voltage-gated K+ channels in the mammalian brain are functionally heterogeneous. Mechanisms which may underlie heterogeneity expression of multiple channel subunit genes, alternative splicing and formation heteromultimers from different subunits. To examine molecular basis regional cell-specific rat situ hybridization techniques were used. The transcript distribution patterns 11 cloned genes encoding both slow- fast-inactivating four gene families examined at stages development. results show...
The isolation and characterization of five clones carrying sequences the α‐, β‐, γ‐, δ‐ ɛ‐subunit precursors rat muscle acetylcholine receptor (AChR) are described. deduced amino acid indicate that these polypeptides contain 457–519 acids reveal structural characteristics common to subunits ligand‐gated ion channels. pattern subunit‐specific mRNA levels in shows characteristic changes during development following denervation, suggesting innervation reduces expression β‐ δ‐subunit mRNAs,...
Multi-transgenic pigs produced for use in xenotransplantation have to be screened the presence and expression of porcine endogenous retroviruses (PERV) select animals with low PERV load. The production transgenic may also associated integration transgene adjacent or into locus a provirus, potentially leading an enhanced virus expression.Non-transgenic animals, single-transgenic, multi-transgenic were PERV-A, -B, -C recombinant PERV-A/C using polymerase chain reaction (PCR). was determined by...
Injection of linearized DNA constructs into the pronuclei fertilized mammalian eggs is a standard method for producing transgenic embryos and animals. Here, we show that injection covalently closed circular (ccc) plasmids cytoplasm bovine murine highly efficient simple alternative ectopic expression foreign in embryos. A broad range could be successfully expressed preimplantation stages, including minicircles with scaffold/matrix attachment region (S/MAR), conventional plasmids, bacterial...