A5038082575- Reproductive Biology and Fertility
- Microtubule and mitosis dynamics
- Pluripotent Stem Cells Research
- Renal and related cancers
- Prenatal Screening and Diagnostics
- CRISPR and Genetic Engineering
- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- Chromosomal and Genetic Variations
- DNA Repair Mechanisms
- RNA and protein synthesis mechanisms
- RNA Interference and Gene Delivery
- Animal Genetics and Reproduction
- Plant Genetic and Mutation Studies
- Molecular Biology Techniques and Applications
- Ubiquitin and proteasome pathways
- Viral Infectious Diseases and Gene Expression in Insects
- Genomic variations and chromosomal abnormalities
- Plant Physiology and Cultivation Studies
- thermodynamics and calorimetric analyses
- Viral Infections and Vectors
- PI3K/AKT/mTOR signaling in cancer
- Wnt/β-catenin signaling in development and cancer
- FOXO transcription factor regulation
Veterinary Research Institute
2014-2025
Czech Academy of Sciences, Institute of Animal Physiology and Genetics
2003-2025
Central European Institute of Technology
2012-2023
Masaryk University
2016-2019
University Hospital Carl Gustav Carus
2014
Czech Academy of Sciences
2013
Institute of Animal Physiology of the Slovak Academy of Sciences
2012
University of Oxford
2008-2009
Research Institute of Molecular Pathology
2006-2009
University of Pennsylvania
2003-2008
The success of somatic nuclear transfer critically depends on the cell cycle stage donor nucleus and recipient cytoplast. In this study we tested serum deprivation as well two reversible inhibitors, aphidicolin butyrolactone I, for their ability to synchronize porcine fetal fibroblasts at either G0 or G1/S G2/M transition. synchronization efficiency various protocols was determined by fluorescence-activated sorting (FACS), proliferation assays, semiquantitative multiplex reverse...
Significance Hybrid sterility contributes to speciation by restricting gene flow between related taxa. Although four hybrid genes have been identified in Drosophila and mouse so far, the underlying molecular mechanisms are largely unknown. We describe extensive asynapsis of chromosomes male female meiosis F1 hybrids two closely subspecies. Using intersubspecific chromosome-substitution strains, we demonstrate that heterospecific pairing homologous is a preexisting condition may represent...
Abstract The fully grown mammalian oocyte is transcriptionally quiescent and utilizes only transcripts synthesized stored during early development. However, we find that an abundant RNA population retained in the nucleus contains specific mRNAs important for meiotic progression. Here show first division, shortly after nuclear envelope breakdown, translational hotspots develop chromosomal area a region was previously surrounded nucleus. These distinct are separated by endoplasmic reticulum...
Proteolytic activity of separase is required for chiasma resolution during meiosis I in mouse oocytes. Rec8, the meiosis-specific alpha-kleisin subunit cohesin, a key target yeast. Is equivalent protein also mammals? We show here that cleaves Rec8 at three positions vitro but only when latter hyper-phosphorylated. Expression variant (Rec8-N) cannot be cleaved these sites causes sterility male mice. Their seminiferous tubules lack normal complement 2 C secondary spermatocytes and 1 spermatids...
Chromosome segregation errors are highly frequent in mammalian female meiosis, and their incidence gradually increases with maternal age. The fate of aneuploid eggs is obviously dependent on the stringency mechanisms for detecting unattached or repairing incorrectly attached kinetochores. In case failure, newly formed embryo will inherit impaired set chromosomes, which have severe consequences its further development. Whether spindle assembly checkpoint (SAC) oocytes capable arresting cell...
Studies in mutant organisms deficient RNA interference (RNAi) and related post-transcriptional gene silencing implicated a role for single class of RNA-dependent polymerases (RdRp). Nevertheless, sequence homologs to these RdRps have not been found coelomate such as Drosophila or mammals. This lack homologous sequences does exclude that an RdRp functions RNAi because could be acquired by horizontal transfer from virus. In fact, is mice (Aquarius) we observe it expressed mouse oocytes early...
Meiotic maturation of mammalian oocytes (transition from prophase I to metaphase II) is accompanied by complex changes in the protein phosphorylation pattern. At least two major kinases are involved these events; namely, cdc2 kinase and mitogen-activated (MAP) kinase, because inhibition arrest germinal vesicle (GV) stage. We show that during meiotic bovine oocytes, translation initiation factor, eIF4E (the cap binding protein), gradually becomes phosphorylated. This substantial begins at...
It is generally accepted that mammalian oocytes are frequently suffering from chromosome segregation errors during meiosis I, which have severe consequences, including pregnancy loss, developmental disorders and mental retardation. In a search for physiologically more relevant model than rodent to study this phenomenon, we employed comparative genomic hybridization (CGH), combined with whole genome amplification (WGA), the frequency of aneuploidy in porcine oocytes, rare cells obtained aged...
A master regulator of DNA replication, CDC6 also functions in the DNA-replication checkpoint by preventing rereplication. Cyclin-dependent kinases (CDKs) regulate amount and localization throughout cell cycle; phosphorylation after replication initiation leads to its proteolysis yeast or translocation cytoplasm mammals. Overexpression during late S phase prevents entry into M activating CHEK1 kinase that then inactivates CDK1/cyclin B, which is essential for G2/M-phase transition. We...
Preimplantation mouse embryo development involves temporal-spatial specification and segregation of three blastocyst cell lineages: trophectoderm, primitive endoderm epiblast. Spatial separation the outer-trophectoderm lineage from two other inner-cell-mass (ICM) lineages starts with 8- to 16-cell transition concludes at 32-cell stages. Accordingly, ICM is derived primary secondary contributed cells; debated relative EPI versus PrE potencies. We report generation but not populations highly...
Proper assembly of the spindle apparatus is crucially important for faithful chromosome segregation during anaphase. Thanks to effort over last decades, we have very detailed information about many events leading and segregation, however still do not understand certain aspects, including, example, length control. When tight regulation size lost, errors emerge. Currently, there are several hypotheses trying explain molecular mechanism The number kinetochores, activity rulers, intracellular...
Chromosome segregation in female germ cells and early embryonic blastomeres is known to be highly prone errors. The resulting aneuploidy therefore the most frequent cause of termination development embryo loss mammals. And specific cases, when actually compatible with fetal development, it leads severe developmental disorders. main surveillance mechanism, which essential for fidelity chromosome segregation, Spindle Assembly Checkpoint (SAC). although all eukaryotic carry genes required SAC,...
Abstract In this study, butyrolactone I (BL I), a potent and specific inhibitor of cyclin‐dependent kinases (cdk), is shown to inhibit germinal vesicle breakdown (GVBD) in pig oocytes. Oocytes treated with 100 μM BL were arrested the (GV)‐stage displayed low activity cdc2 kinase MAP kinase. Nevertheless, chromosome condensation occurred highly condensed bivalents seen within an intact GV after 24‐hr culture presence I. The inhibitory effect on activation during was likely mediated through...
The step-wise assembly of a functional nucleolus, which occurs over the first few cell cycles during preimplantation development, is poorly understood. In this study, we examined function evolutionary conserved nucleolar protein SURF6 in mouse embryo development. Immunocytochemical analyses revealed that localization was similar but not identical to those fibrillarin and B23/nucleophosmin 1, are involved rRNA processing ribosome biogenesis mammalian somatic cells. Surf6 mRNA, expressed...
Enzymes of the Polo-like kinase (Plk) family are active in pathways controlling mitosis several species. We have cloned cDNA fragments porcine homologues Plk1, Plk2, and Plk3 employing fetal fibroblasts as source. All three partial cDNAs showed high sequence homology with their mouse human counterparts contained Polo box, a domain characteristic for all kinases. The expression levels Plk1 mRNA at various points cell cycle synchronized were analyzed by both RT-PCR ribonuclease protection...
Vibrational spectroscopy enables the label-free characterization of cells and tissue by probing biochemical composition. Here, we evaluated these techniques to identify glioblastoma stem cells.The fingerprints were established in human cell lines with high low content CD133 (cluster differentiation 133)-positive using attenuated total reflection Fourier-transform infrared (ATR FT-IR) on vital FT-IR mapping, which delivers spatially resolved spectroscopic datasets. After data preprocessing,...