A5038167475- Microtubule and mitosis dynamics
- Genomics and Chromatin Dynamics
- Reproductive Biology and Fertility
- DNA Repair Mechanisms
- Prenatal Screening and Diagnostics
- Epigenetics and DNA Methylation
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Fungal and yeast genetics research
- Pluripotent Stem Cells Research
- Vitamin C and Antioxidants Research
- CRISPR and Genetic Engineering
- Ubiquitin and proteasome pathways
- Kidney Stones and Urolithiasis Treatments
- Renal and related cancers
- BRCA gene mutations in cancer
- Chromosomal and Genetic Variations
- Pediatric Urology and Nephrology Studies
- Genetics, Bioinformatics, and Biomedical Research
- Geotechnical Engineering and Analysis
- Plant Gene Expression Analysis
- Protist diversity and phylogeny
- Tunneling and Rock Mechanics
- Abdominal Trauma and Injuries
- Data-Driven Disease Surveillance
Université Paris Cité
2012-2024
Institut Jacques Monod
2022-2024
Centre National de la Recherche Scientifique
2013-2024
Laboratoire de Biologie du Développement
2004-2024
Sorbonne Université
2012-2023
Institut de Biologie Paris-Seine
2015-2023
Centre National pour la Recherche Scientifique et Technique (CNRST)
2013
Inserm
2007-2008
Witten/Herdecke University
2005
Memorial Sloan Kettering Cancer Center
1998-2003
In female meiosis, chromosome missegregations lead to the generation of aneuploid oocytes and can cause development trisomies or infertility. Because mammalian meiosis I is error prone, full functionality control mechanisms, such as spindle assembly checkpoint (SAC), has been put into question. The SAC monitors correct orientation, microtubule occupancy tension on proteinaceous structures named kinetochores. Although it shown previously that exists in I, where attachments are monopolar, role...
In the budding yeast Saccharomyces cerevisiae, MCM1 encodes an essential DNA-binding protein that regulates transcription of many genes in cooperation with different associated factors. With help a conditional expression system, we show Mcm1 depletion has distinct effect on cell cycle progression by preventing cells from undergoing mitosis. Genes normally exhibit G2-to-M-phase-specific pattern, such as CLB1, CLB2, CDC5, SWI5, and ACE2, remain uninduced absence functional Mcm1. vivo...
The spindle assembly checkpoint (SAC) ensures correct separation of sister chromatids in somatic cells and provokes a cell cycle arrest metaphase if one chromatid is not correctly attached to the bipolar spindle. Prolonged due overexpression Mad2 has been shown be deleterious ensuing anaphase, leading generation aneuploidies tumorigenesis. Additionally, some SAC components are essential for timing prometaphase. In meiosis, we others have previously that Mad2-dependent functional during first...
Cell cycle control is modified at meiosis compared to mitosis, because two divisions follow a single DNA replication event. Cyclin-dependent kinases (CDKs) promote progression through both and central regulator of their activity the APC/C (Anaphase Promoting Complex/Cyclosome) that especially required for exit from mitosis. We have shown previously OSD1 involved in entry into I II Arabidopsis thaliana; however, molecular mechanism by which controls these transitions has remained unclear....
Mammalian female meiosis is error prone, with rates of meiotic chromosome missegregations strongly increasing towards the end reproductive lifespan. A strong reduction BubR1 has been observed in oocytes women approaching menopause and ovaries aged mice, which led to hypothesis that a gradual decline contributes age-related aneuploidization. Here we employ conditional knockout approach mouse dissect roles BubR1. We show required for diverse functions, including persistent spindle assembly...
Activation of the mitotic checkpoint pathway in response to spindle damage eukaryotic cells delays exit from mitosis an attempt prevent chromosome missegregation. One component this pathway, hsMad2, has been shown mammalian physically associate with components a ubiquitin ligase activity (termed anaphase promoting complex or APC) when is activated, thereby preventing degradation inhibitors machinery. In present report, we demonstrate that inhibitory association between Mad2 and APC Cdc27...
A key feature of meiosis is the step-wise removal cohesin, protein complex holding sister chromatids together, first from arms in I and then centromere region II. Centromeric cohesin protected by Sgo2 Separase-mediated cleavage, order to maintain together until their separation Failures result aneuploid gametes, preventing generation healthy embryos. Here, we report that kinase activities Bub1 Mps1 are required for localisation region. inhibitor-treated oocytes defective centromeric...
Meiosis poses unique challenges because two rounds of chromosome segregation must be executed without intervening DNA replication. Mammalian cells express numerous temporally regulated cyclins, but how these proteins collaborate to control meiosis remains poorly understood. Here, we show that female mice genetically ablated for cyclin B3 are viable—indicating the protein is dispensable mitotic divisions—but sterile. Mutant oocytes appear normal until metaphase I then display a highly...
Abstract Exit from mitosis is brought about by dramatic changes in the phosphoproteome landscape. A drop Cyclin-dependent kinase (Cdk) activity, master regulatory kinase, and activation of counteracting phosphatases such as Cdc14 budding yeast, results ordered substrate dephosphorylation, allowing entry into a new cell cycle replication licensing. In meiosis however, two divisions have to be executed without intermediate DNA replication, implying that global phosphorylation dephosphorylation...
The meiotic segregation pattern to generate haploid gametes is mediated by step-wise cohesion removal separase, first from chromosome arms in meiosis I, and then the centromere region II. In mammalian oocytes, separase tightly controlled during hours-long prometaphase until activated for a short time window, again inhibited metaphase II arrest lifted fertilization. Centromeric cohesin protected cleavage Sgo2-PP2A I. It remained enigmatic how tight control of alternating activation...
In the presented work we aimed at improving confocal imaging to obtain highest possible resolution in thick biological samples, such as mouse oocyte. We therefore developed an image processing workflow that allows lateral and axial of a standard microscope. Our comprises refractive index matching, optimization microscope hardware parameters restoration by deconvolution. compare two different deconvolution algorithms, evaluate necessity denoising establish optimal procedure. validate our sub...
In meiosis, two specialized cell divisions allow the separation of paired chromosomes first, then sister chromatids. Separase removes cohesin complex holding chromatids together in a stepwise manner from chromosome arms meiosis I, centromere region II. Using mouse oocytes, our study reveals that cyclin A2 promotes entry into as well an additional unexpected role; namely, its requirement for separase-dependent chromatid Untimely A2-associated kinase activity I leads to precocious separation,...
Article1 March 2021Open Access Transparent process Kinetochore individualization in meiosis I is required for centromeric cohesin removal II Yulia Gryaznova Institut de Biologie Paris Seine, Sorbonne Université, Paris, France CNRS UMR7622 Developmental Biology Lab, Search more papers by this author Leonor Keating orcid.org/0000-0002-8493-9458 FranceThese authors contributed equally to work Sandra A Touati orcid.org/0000-0002-0007-2130 Damien Cladière Warif El Yakoubi Eulalie Buffin Katja...
Regulated conformational changes of proteins are critical for cellular signal transduction. The spindle checkpoint protein Mad2 is an unusual with two native folds: the latent open conformer (O-Mad2) and activated closed (C-Mad2). During mitosis, cytosolic O-Mad2 binds to Mad1–Mad2 core complex at unattached kinetochores undergoes activation become C-Mad2. C-Mad2 inhibits Cdc20, activator APC/C, prevent precocious anaphase onset. Here, we show that transition regulated by phosphorylation...