A5018688657- CAR-T cell therapy research
- Acute Lymphoblastic Leukemia research
- Protein purification and stability
- Mass Spectrometry Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- Analytical Chemistry and Chromatography
- Microtubule and mitosis dynamics
- RNA Research and Splicing
- RNA Interference and Gene Delivery
- DNA Repair Mechanisms
- Nuclear Structure and Function
- Advanced Proteomics Techniques and Applications
- Glycosylation and Glycoproteins Research
- Protist diversity and phylogeny
- Chromosomal and Genetic Variations
- Genomics and Phylogenetic Studies
- Protein Interaction Studies and Fluorescence Analysis
- Metabolomics and Mass Spectrometry Studies
- Hemophilia Treatment and Research
- Microfluidic and Capillary Electrophoresis Applications
- Microbial Community Ecology and Physiology
- Ubiquitin and proteasome pathways
- Viral Infections and Vectors
- Genetics and Neurodevelopmental Disorders
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
Université Paris Cité
2021-2025
Institut Jacques Monod
2021-2025
Centre National de la Recherche Scientifique
2004-2025
LFB (France)
2011-2021
Université de Strasbourg
2011
Institut Curie
2004-2008
Institut Pluridisciplinaire Hubert Curien
2008
Numerous putative post-translational modifications may induce variations of monoclonal antibodies charge distribution that can potentially affect their biological activity. The characterization and the monitoring these variants are critical quality requirements to ensure stability process consistency. Charge usually characterized by preparative ion exchange chromatography, collection fractions subsequent reverse-phase liquid chromatography with mass spectrometry analysis. While this be...
Viruses must overcome the interferon-mediated antiviral response to replicate and propagate into their host. Rabies virus (RABV) phosphoprotein P is known inhibit interferon induction. Here, using a global mass spectrometry approach, we show that RABV binds TBK1, kinase located at crossroads of many induction pathways, resulting in innate immunity inhibition. Mutations TBK1 phosphorylation sites abolish binding. Importantly, demonstrate upon infection or detection dsRNA by sensors, its...
In metazoa, cilia assembly is a cellular process that starts with centriole to basal body maturation, migration the cell surface, and docking plasma membrane. Basal involves interaction of both distal end transition fibers/distal appendages, Mutations in numerous genes involved zone are associated most severe ciliopathies, highlighting importance these events cilium biogenesis. this context, ciliate Paramecium has been widely used as model system study assembly. However, despite evolutionary...
Intracellular pathogens develop elaborate mechanisms to survive within the hostile environments of host cells. Theileria parasites infect bovine leukocytes and cause devastating diseases in cattle developing countries. spp. have evolved sophisticated strategies hijack leukocytes, inducing proliferative invasive phenotypes characteristic cell transformation. secrete proteins into recruit induce oncogenic signaling for parasite survival. It is unknown how evade defense mechanisms, such as...
Myosin VI contains an inserted sequence that is unique among myosin superfamily members and has been suggested to be a determinant of the reverse directionality unusual motility motor. It thought each head two-headed molecule binds one calmodulin (CaM) by means single “IQ motif”. Using truncations protein electrospray ionization(ESI)-MS, we demonstrate in fact two CaMs. One CaM conventional IQ motif either with or without calcium likely plays regulatory role when its N-terminal lobe. The...
Despite significant analytical improvements during this last decade, characterizing the whole integrity of monoclonal antibodies their bioproduction remains a challenge. In study, we report new approach to evaluate overall heterogeneity/integrity mAbs by LC-MS after combined proteolysis at lower- and upper-hinge sites using immunoglobulin-degrading enzymes IdeS IgdE respectively. The sample preparation did not use any harsh conditions such as low pH, high temperature or reductive enables...
Abstract Exit from mitosis is brought about by dramatic changes in the phosphoproteome landscape. A drop Cyclin-dependent kinase (Cdk) activity, master regulatory kinase, and activation of counteracting phosphatases such as Cdc14 budding yeast, results ordered substrate dephosphorylation, allowing entry into a new cell cycle replication licensing. In meiosis however, two divisions have to be executed without intermediate DNA replication, implying that global phosphorylation dephosphorylation...
<p>Supplementary Figure S5. The CD40 agonist does not bind to mouse immune cells <i>in vivo</i>.</p>
<div>AbstractPurpose:<p>Cluster of differentiation 40 (CD40) is expressed on B-cell acute lymphoblastic leukemia (B-ALL) cases. However, the effect CD40 activation B-ALL cells has never been tested <i>in vivo</i>.</p>Experimental Design:<p>The aim our preclinical study was to investigate therapeutic potential a agonist in treatment using patient-derived xenograft mouse models.</p>Results:<p>Intravenous administration significantly impeded cell...
<p>Supplementary Figure S4. Agonist CD40 binds primary B-ALL cells.</p>
<p>Supplementary Figure S9. Combination of CD40 agonist and chemotherapy significantly reduces B-ALL development in PDX mice.</p>
<p>Supplementary Figure S6. The CD40 agonist does not activate NFκB on B-ALL cells <i>in vivo</i>.</p>
<p>Supplementary Figure S8. The expression of specific markers remains unchanged on residual B-ALL cells following treatment with the CD40 agonist.</p>
<p>Supplementary Figure S2. Full-length western blots.</p>
<p>Supplementary Figure S7. Molecular markers expressed by B-ALL PDX models.</p>
<p>Supplementary Figure S1. Flow cytometry plots at diagnosis on B-ALL samples used to generate PDX mice.</p>
<p>Supplementary Figure S3. <i>CD40</i> transcription on B-ALL primary samples.</p>