A5074746771- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- Cancer-related Molecular Pathways
- Gene expression and cancer classification
- Animal Genetics and Reproduction
- RNA and protein synthesis mechanisms
- Chromosomal and Genetic Variations
- Molecular Biology Techniques and Applications
- Virus-based gene therapy research
- Pluripotent Stem Cells Research
- Mitochondrial Function and Pathology
- DNA Repair Mechanisms
- RNA Interference and Gene Delivery
- Ubiquitin and proteasome pathways
- Cell Adhesion Molecules Research
- Genomics and Phylogenetic Studies
- RNA modifications and cancer
- Genetics, Bioinformatics, and Biomedical Research
- Cancer-related gene regulation
- Telomeres, Telomerase, and Senescence
- RNA Research and Splicing
- Signaling Pathways in Disease
- Bioinformatics and Genomic Networks
- Genetics, Aging, and Longevity in Model Organisms
Université Paris Cité
2013-2024
Centre National de la Recherche Scientifique
2014-2024
Epigénétique et Destin Cellulaire
2013-2024
Institut Pasteur
2000-2023
Institut Necker Enfants Malades
2023
Sorbonne Paris Cité
2011-2019
Institut Jacques Monod
2015
Délégation Paris 7
2006-2014
Université de Bourgogne
2011
Institut Cochin
2011
Abstract Upregulation of the matrix metalloproteinase (MMP)–9 plays a central role in tumor progression and metastasis by stimulating cell migration, invasion, angiogenesis. To gain insights into MMP-9 expression, we investigated its epigenetic control reversible model cancer that is initiated infection with intracellular Theileria parasites. Gene induction parasite was associated trimethylation histone H3K4 (H3K4me3) at promoter. Notably, found methyltransferase SMYD3 only upregulated upon...
To assess directly the functional role of integrin VLA-3 (alpha 3 beta 1), we transfected human alpha cDNA into erythroleukemia (K562) cells and rhabdomyosarcoma (RD) cells. The resulting transfectants (KA3 RA3) expressed 1 on cell surface as confirmed using a panel nine anti-alpha monoclonal antibodies. Neither exhibited increased adhesion to extracellular matrix proteins fibronectin, laminin, collagen. However, KA3 did bind strongly deposited by epidermal carcinoma lines, allowing attach...
ABSTRACT JunD is one of three mammalian Jun proteins that contribute to the AP-1 transcription factor complex. Distinct regulation and functions have been proposed for each member, but less known about biological these in vivo. To investigate role JunD, we inactivated murine gene by replacement with a bacterial lacZ reporter gene. Embryonic expression was initially detected developing heart cardiovascular system. Subsequent broadening phases were observed during embryonic development adult...
Different components of the AP1 transcription factor complex appear to have distinct effects on cell proliferation and transformation. In contrast other components, JunD has been shown inhibit proliferation. Also, in prior studies, alone bound menin, product MEN1 tumor suppressor gene, JunD's transcriptional activity was inhibited by suggesting that might achieve all or most its unique properties through binding menin. Analyses menin proliferation, morphology, cyclin D1 stable lines unmasked...
Theileria parasites invade and transform bovine leukocytes causing either East Coast fever (T. parva), or tropical theileriosis annulata). Susceptible animals usually die within weeks of infection, but indigenous infected cattle show markedly reduced pathology, suggesting that host genetic factors may cause disease susceptibility. Attenuated live vaccines are widely used to control attenuation is associated with invasiveness macrophages in vitro. Disease pathogenesis therefore linked...
The intracellular parasite Theileria is the only eukaryote known to transform its mammalian host cells. We investigated mechanisms involved in parasite-induced transformation phenotypes. Tumour progression a multistep process, yet 'oncogene addiction' implies that cancer cell growth and survival can be impaired by inactivating single gene, offering rationale for targeted molecular therapies. Furthermore, feedback loops often act as key regulatory hubs tumorigenesis. searched microRNAs...
Abstract The transcriptional activity of the p53 protein is central to its role in tumor suppression. Identification complete repertoire p53-regulated genes critical for dissecting complexity network. Although several different approaches have been used characterize genetic program, we still lack a comprehensive molecular understanding how prevents cancer. Using computational approach, generated genome-wide map binding sites (p53BS) identify novel target genes. We show that presence nearby...
Intracellular pathogens develop elaborate mechanisms to survive within the hostile environments of host cells. Theileria parasites infect bovine leukocytes and cause devastating diseases in cattle developing countries. spp. have evolved sophisticated strategies hijack leukocytes, inducing proliferative invasive phenotypes characteristic cell transformation. secrete proteins into recruit induce oncogenic signaling for parasite survival. It is unknown how evade defense mechanisms, such as...
The Jun family of activator protein 1 (AP-1) transcription factors (c-Jun, JunB, and JunD) is involved in fundamental biological processes such as proliferation, apoptosis, tumor angiogenesis, hypertrophy. role individual AP-1 the stressed heart not clear. In present study we analyzed JunD survival, hypertrophy, angiogenesis pressure-overloaded mouse after thoracic aortic constriction.Mice lacking (knockout [KO]) showed increased mortality enhanced cardiomyocyte apoptosis fibrosis associated...
Abstract Metabolic reprogramming is an important feature of host–pathogen interactions and a hallmark tumorigenesis. The intracellular apicomplexa parasite Theileria induces Warburg-like effect in host leukocytes by hijacking signaling machineries, epigenetic regulators transcriptional programs to create transformed cell state. molecular mechanisms underlying transformation are unclear. Here we show that parasite-encoded prolyl-isomerase, TaPin1, stabilizes pyruvate kinase isoform M2 (PKM2)...
ABSTRACT Angiogenesis and the formation of new blood vessels requires coordinated regulation matrix proteolysis endothelial cell migration. Cellular proteolytic capacity is balance between secreted metalloproteinases (MMP) their inhibitors (TIMPs). We have examined gelatinase/TIMP by transforming growth factor-β1 (TGF-β1) phorbol myristate acetate (PMA) in bovine cells. The low constitutive expression gelatinase A/MMP-2 was upregulated TGF-β1 a dose-dependent manner. Gelatinase B/MMP-9 only...
The AP-1 transcription factor, composed of Jun and Fos proteins, plays a crucial role in the fine tuning cell proliferation. We showed previously that complexes are activated during proliferative response parallels development renal lesions after nephron reduction, but little is known about specific individual Jun/Fos components deterioration process. Here we used JunD knockout (JunD–/–) mice an experimental model chronic injury (75% reduction) to explore JunD. Nephron reduction resulted...
We have studied the gene of human β2 integrin subunit (CD18) and found it to be organised into 16 exons spanning a region about 40 kb. All exon/intron boundaries conform GT/AG splicing consensus. The coding for cysteine‐rich region, which has been postulated consist 3 or 4 repeating elements, are not correspondingly. Transcription initiates from multiple sites may due absence an upstream TATA box. polyadenylation site is also heterogeneous. Five different were identified over stretch 10 bases.