A5044284421- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Plant tissue culture and regeneration
- Photosynthetic Processes and Mechanisms
- Microtubule and mitosis dynamics
- Plant Reproductive Biology
- Diatoms and Algae Research
- DNA Repair Mechanisms
- Plant Genetic and Mutation Studies
- Protist diversity and phylogeny
- Ubiquitin and proteasome pathways
- Microbial Community Ecology and Physiology
- Plant Gene Expression Analysis
- Chromosomal and Genetic Variations
- Light effects on plants
- Genomics and Phylogenetic Studies
- Plant Physiology and Cultivation Studies
- Plant Stress Responses and Tolerance
- Plant-Microbe Interactions and Immunity
- Cancer-related Molecular Pathways
- Genomics and Chromatin Dynamics
- Nematode management and characterization studies
- Aluminum toxicity and tolerance in plants and animals
- Legume Nitrogen Fixing Symbiosis
- Plant Disease Resistance and Genetics
Ghent University
2016-2025
VIB-UGent Center for Plant Systems Biology
2016-2025
Vlaams Instituut voor Biotechnologie
2009-2023
Freie Universität Berlin
2023
Ghent University Hospital
2012
University of Antwerp
2009-2011
Laboratoire d'Écophysiologie Moléculaire des Plantes sous Stress Environnementaux
2011
Ithaca College
2011
Institute of Plant Biology
2011
HUN-REN Szegedi Biológiai Kutatóközpont
2011
Cyclin-dependent kinase inhibitors, such as the mammalian p27Kip1 protein, regulate correct cell cycle progression and integration of developmental signals with core machinery. These inhibitors have been described in plants, but their function remains unresolved. We isolated seven genes from Arabidopsis that encode proteins distant sequence homology p27Kip1, designated Kip-related (KRPs). The KRPs were characterized by domain organization transcript profiles. With exception KRP5, all...
Cyclin-dependent kinases and cyclins regulate with the help of different interacting proteins progression through eukaryotic cell cycle. A high-quality, homology-based annotation protocol was applied to determine core cycle genes in recently completed Arabidopsis genome sequence. In total, 61 were identified belonging seven selected families regulators, for which 30 are new or corrections existing annotation. class putative regulators found that probably competitors E2F/DP transcription...
Cell proliferation is the main driving force for plant growth. Although genome sequence analysis revealed a high number of cell cycle genes in plants, little known about molecular complexes steering division. In targeted proteomics approach, we mapped core complex machinery at heart Arabidopsis thaliana control. Besides central regulatory network complexes, distinguished peripheral that links to up‐ and downstream pathways. Over 100 new candidate proteins were predicted an in‐depth...
The quiescent center (QC) plays an essential role during root development by creating a microenvironment that preserves the stem cell fate of its surrounding cells. Despite being surrounded highly mitotic active cells, QC cells self-renew at low proliferation rate. Here, we identified ERF115 transcription factor as rate-limiting division, acting transcriptional activator phytosulfokine PSK5 peptide hormone. marks division but is restrained through proteolysis APC/C(CCS52A2) ubiquitin ligase,...
Wounding is a primary trigger of organ regeneration, but how wound stress reactivates cell proliferation and promotes cellular reprogramming remains elusive. In this study, we combined transcriptome analysis with quantitative hormonal to investigate wounding induces callus formation in Arabidopsis (Arabidopsis thaliana). Our time course RNA-seq revealed that dynamic transcriptional changes, starting from rapid responses followed by the activation metabolic processes protein synthesis...
Cell division is one of the most conspicuous features life, and thus several elements control cell are common to both prokaryotes eukaryotes ([Amon, 1998][1]; [Leatherwood, 1998][2]). The degree evolutionary conservation especially striking among eukaryotes, where
Abstract Upon the incidence of DNA stress, ataxia telangiectasia–mutated (ATM) and Rad3-related (ATR) signaling kinases activate a transient cell cycle arrest that allows cells to repair before proceeding into mitosis. Although ATM-ATR pathway is highly conserved over species, mechanisms by which plant stop their in response loss genome integrity are unclear. We demonstrate regulatory WEE1 kinase gene Arabidopsis thaliana transcriptionally activated upon cessation replication or damage an...
Transgenic Arabidopsis thaliana plants overproducing the E2Fa-DPa transcription factor have two distinct cell-specific phenotypes: some cells divide ectopically and others are stimulated to endocycle. The decision of undergo extra mitotic divisions has been postulated depend on presence a mitosis-inducing (MIF). Plants possess unique class cyclin-dependent kinases (CDKs; B-type) for which no ortholog is found in other kingdoms. peak CDKB1;1 activity around G2-M boundary suggested that it...
Abstract Exit from the mitotic cell cycle and initiation of differentiation frequently coincides with onset endoreduplication, a modified during which DNA continues to be duplicated in absence mitosis. Although endoreduplication share much same machinery, regulatory mechanisms controlling transition between both cycles remain poorly understood. We show that A-type cyclin-dependent kinase CDKA;1 its specific inhibitor, Kip-related protein, KRP2 regulate mitosis-to-endocycle Arabidopsis...
Abstract Organ growth results from the progression of component cells through subsequent phases proliferation and expansion before reaching maturity. We combined kinematic analysis, flowcytometry, microarray analysis to characterize cell cycle regulation during process leaves 1 2 Arabidopsis (Arabidopsis thaliana). Kinematic showed that epidermis proliferates until day 12; thereafter, expand 19 when reach Flowcytometry revealed endoreduplication occurs time division rates decline end...
Recessive mutations in the SIAMESE (SIM) gene of Arabidopsis thaliana result multicellular trichomes harboring individual nuclei with a low ploidy level, phenotype strikingly different from that wild-type trichomes, which are single cells nuclear DNA content approximately 16C to 32C. These observations suggested SIM is required suppress mitosis as part switch endoreplication trichomes. Here, we demonstrate encodes nuclear-localized 14-kD protein containing cyclin binding motif and found...
Abstract Entry into the S phase of cell cycle is controlled by E2F transcription factors that induce genes required for progression and DNA replication. Although pathway highly conserved in higher eukaryotes, only a few target have been experimentally validated plants. We combined microarray analysis bioinformatics tools to identify plant E2F-responsive genes. Promoter regions were induced at transcriptional level Arabidopsis (Arabidopsis thaliana) seedlings ectopically expressing E2Fa DPa...
Abstract Analysis of gene expression data generated by high-throughput microarray transcript profiling experiments has demonstrated that genes with an overall similar pattern are often enriched for functions. This guilt-by-association principle can be applied to define modular programs, identify cis-regulatory elements, or predict functions unknown based on their coexpression neighborhood. We evaluated the potential use Gene Ontology (GO) enrichment a gene's neighborhood as tool its function...
Abstract The molecular mechanisms by which the phytohormone auxin coordinates cell division with growth and differentiation are largely unknown. Here, we show that in Arabidopsis thaliana E2FB, accumulation stability positively regulated auxin. Coexpression of but not E2FA, its dimerization partner A, stimulated proliferation absence tobacco (Nicotiana tabacum) Bright Yellow-2 cells. E2FB entry into both S- M-phases, latter corresponding to activation a plant-specific mitotic regulator,...
Abstract Multicellular organisms depend on cell production, fate specification, and correct patterning to shape their adult body. In plants, auxin plays a prominent role in the timely coordination of these different cellular processes. A well-studied example is lateral root initiation, which triggers founder specification cycle activation xylem pole–positioned pericycle cells. Here, we report that E2Fa transcription factor Arabidopsis thaliana an essential component regulates asymmetric...
Abstract The mitosis-to-endocycle transition requires the controlled inactivation of M phase-associated cyclin-dependent kinase (CDK) activity. Previously, B-type CDKB1;1 was identified as an important negative regulator endocycle onset. Here, we demonstrate that copurifies and associates with A2-type cyclin CYCA2;3. Coexpression CYCA2;3 triggered ectopic cell divisions inhibited endoreduplication. Moreover, enhanced endoreduplication phenotype observed after overexpression a...