A5053779658- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- Plant Pathogens and Resistance
- Plant Molecular Biology Research
- Plant Pathogens and Fungal Diseases
- Plant pathogens and resistance mechanisms
- Photosynthetic Processes and Mechanisms
- Plant Virus Research Studies
- Plant Parasitism and Resistance
- Plant Disease Resistance and Genetics
- Algal biology and biofuel production
- Antioxidant Activity and Oxidative Stress
- Plant Pathogenic Bacteria Studies
- Nematode management and characterization studies
- Plant and Fungal Interactions Research
- Plant nutrient uptake and metabolism
- Plant responses to elevated CO2
- Microtubule and mitosis dynamics
- Insect symbiosis and bacterial influences
- Plant and animal studies
- Chromosomal and Genetic Variations
- Phytase and its Applications
- Plant Reproductive Biology
- CRISPR and Genetic Engineering
- Plant tissue culture and regeneration
Justus-Liebig-Universität Gießen
2015-2024
University of Strathclyde
2022
University of California, Berkeley
2022
Huazhong Agricultural University
2022
State Forestry and Grassland Administration
2022
University of Warwick
2010-2021
Center for Free-Electron Laser Science
2012
Universität Hamburg
2012
University of Konstanz
2001-2006
An analysis of protein-protein interactions in Arabidopsis identifies the plant interactome.
Cell proliferation is the main driving force for plant growth. Although genome sequence analysis revealed a high number of cell cycle genes in plants, little known about molecular complexes steering division. In targeted proteomics approach, we mapped core complex machinery at heart Arabidopsis thaliana control. Besides central regulatory network complexes, distinguished peripheral that links to up‐ and downstream pathways. Over 100 new candidate proteins were predicted an in‐depth...
ABSTRACT Astaxanthin is a high-value carotenoid which used as pigmentation source in fish aquaculture. Additionally, beneficial role of astaxanthin food supplement for humans has been suggested. The unicellular alga Haematococcus pluvialis suitable biological production. In the context strong biotechnological relevance H. , we developed genetic transformation protocol metabolic engineering this green alga. First, gene coding biosynthesis enzyme phytoene desaturase was isolated from and...
Oomycete pathogens cause diverse plant diseases. To successfully colonize their hosts, they deliver a suite of effector proteins that can attenuate defenses. In the oomycete downy mildews, effectors carry signal peptide and an RxLR motif. Hyaloperonospora arabidopsidis (Hpa) causes mildew on model Arabidopsis thaliana (Arabidopsis). We investigated if candidate predicted in genome sequence Hpa isolate Emoy2 (HaRxLs) were able to manipulate host defenses different accessions. developed rapid...
Abstract Astaxanthin is a high-value carotenoid used as pigmentation source in fish aquaculture. In addition, beneficial role of astaxanthin food supplement for humans becoming evident. The unicellular green alga Haematococcus pluvialis seems to be suitable natural astaxanthin. accumulation H. occurs response environmental stress such high light and salt stress. Here, the isolation biosynthesis gene phytoene synthase reported. Furthermore, expression hydroxylase, two key enzymes...
Summary Filamentous phytopathogens form sophisticated intracellular feeding structures called haustoria in plant cells. Pathogen effectors are likely to play a role the establishment and maintenance of addition their better‐characterized suppressing defence. However, specific mechanisms by which these promote virulence remain unclear. To address this question, we examined changes subcellular architecture using live‐cell imaging during compatible interaction between oomycete Hyaloperonospora...
Transcriptional reprogramming is integral to effective plant defense. Pathogen effectors act transcriptionally and posttranscriptionally suppress defense responses. A major challenge understanding disease responses discriminating between transcriptional associated with microbial-associated molecular pattern (MAMP)-triggered immunity (MTI) that orchestrated by effectors. high-resolution time course of genome-wide expression changes following Pseudomonas syringae pv tomato DC3000 the...
Summary P seudomonas syringae type III effectors are known to suppress plant immunity promote bacterial virulence. However, the activities and targets of these not well understood. We used genetic, molecular, cell biology methods characterize activities, localization, target H op D 1 effector in A rabidopsis. contributes . virulence rabidopsis reduces effector‐triggered ( ETI ) responses but pathogen‐associated molecular pattern‐triggered PTI responses. Plants expressing supported increased...
Pathogenic and mutualistic microbes actively suppress plant defense by secreting effector proteins to manipulate the host responses for their own benefit. Current knowledge about fungal effectors has been mainly derived from biotrophic hemibiotrophic pathogenic fungi oomycetes with restricted range. We studied colonization strategies of root endophytic basidiomycete Piriformospora indica that colonizes a wide range species thereby establishing long-term relationships. The release P. indica's...
Hyaloperonospora arabidopsidis (Hpa) is an oomycete pathogen causing Arabidopsis downy mildew. Effector proteins secreted from the into plant play key roles in promoting infection by suppressing immunity and manipulating host to pathogen’s advantage. One class of effectors share a conserved ‘RxLR’ motif critical for their translocation cell. Here we characterize interaction between RxLR effector, HaRxL21 (RxL21), transcriptional co-repressor Topless (TPL). We establish that RxL21 TPL...
The Microrchidia (MORC) family proteins are important nuclear regulators in both animals and plants with critical roles epigenetic gene silencing genome stabilization. In the crop plant barley (Hordeum vulgare), seven MORC members have been described. While HvMORC1 has functionally characterized, very little information is available about other HvMORC paralogs. this study, we elucidate role of HvMORC6a its potential interactors regulating immunity via analysis CRISPR/SpCas9-mediated single...
Plants are resistant to most microbial species due nonhost resistance (NHR), providing broad-spectrum and durable immunity. However, the molecular components contributing NHR poorly characterised. We address question of whether failure pathogen effectors manipulate plants plays a critical role in NHR. RxLR (Arg-any amino acid-Leu-Arg) from two oomycete pathogens,
In filamentous fungi, gene silencing by RNA interference (RNAi) shapes many biological processes, including pathogenicity. Recently, fungal small RNAs (sRNAs) have been shown to act as effectors that disrupt activity in interacting plant hosts, thereby undermining their defence responses. We show here the devastating mycotoxin-producing ascomycete Fusarium graminearum (Fg) utilizes DICER-like (DCL)-dependent sRNAs target genes two Poaceae barley (Hordeum vulgare, Hv) and Brachypodium...
Abstract Background Beneficial associations between plants and microbes are widespread in nature have been studied extensively the microbial-dominant environment of rhizosphere. Such highly advantageous for organisms involved, benefiting soil by providing them access to plant metabolites, while growth development enhanced through promotion nutrient uptake and/or protection against (a)biotic stresses. While establishment maintenance mutualistic shown require genetic epigenetic reprogramming,...
Abstract Bidirectional communication between pathogenic microbes and their plant hosts via small (s)RNA-mediated cross-kingdom RNA interference (ckRNAi) is a key element for successful host colonisation. Whether mutualistic fungi of the Serendipitaceae family, known extremely broad range, use sRNAs to colonize roots still under debate. To address this question, we developed pipeline validate accumulation, translocation, activity fungal in post-transcriptional silencing Arabidopsis thaliana...
Abstract Hyaloperonospora arabidopsidis ( Hpa ) is an oomycete pathogen causing Arabidopsis downy mildew. Effector proteins secreted from the into plant play key roles in promoting infection by suppressing immunity and manipulating host to pathogen’s advantage. One class of effectors share a conserved ‘RxLR’ motif critical for their translocation cell. Here we characterize interaction between RxLR effector, HaRxL21 (RxL21), transcriptional co-repressor Topless (TPL). We establish that RxL21...
Abstract In filamentous fungi, gene silencing by RNA interference (RNAi) shapes many biological processes, including pathogenicity. Recently, fungal small RNAs (sRNAs) have been shown to act as effectors that disrupt activity in interacting plant hosts, thereby undermining their defence responses. We show here the devastating mycotoxin-producing ascomycete Fusarium graminearum ( Fg ) utilizes DICER-like (DCL)-dependent sRNAs target genes two Poaceae barley Hordeum vulgare Hv and Brachypodium...
ABSTRACT RNA interference (RNAi) is a crucial mechanism that can contribute to immunity against infectious microbes through the action of DICER-LIKE (DCL) and ARGONAUTE (AGO) proteins. In case fungal pathogen Botrytis cinerea oomycete Hyaloperonospora arabidopsidis , plant DCL AGO proteins have proven roles as negative regulators immunity, suggesting functional specialization these To address this aspect in broader taxonomic context, we characterized colonization pattern an informative set...
Abstract RNA interference (RNAi) is a crucial mechanism in immunity against infectious microbes through the action of DICER‐LIKE (DCL) and ARGONAUTE (AGO) proteins. In case taxonomically diverse fungal pathogen Botrytis cinerea oomycete Hyaloperonospora arabidopsidis , plant DCL AGO proteins have proven roles as negative regulators immunity, suggesting functional specialization these To address this aspect broader taxonomic context, we characterized colonization pattern an informative set...