A5101986075- RNA Research and Splicing
- Sperm and Testicular Function
- Reproductive Biology and Fertility
- RNA modifications and cancer
- Animal Genetics and Reproduction
- Molecular Biology Techniques and Applications
- Gene expression and cancer classification
- Cancer, Hypoxia, and Metabolism
- Genetic and phenotypic traits in livestock
- Circular RNAs in diseases
- RNA and protein synthesis mechanisms
- Extracellular vesicles in disease
- Lipid metabolism and disorders
- Cancer-related molecular mechanisms research
- Virus-based gene therapy research
- Plant pathogens and resistance mechanisms
- Genomics and Phylogenetic Studies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Potato Plant Research
- Cancer Research and Treatments
- Reproductive Physiology in Livestock
- Testicular diseases and treatments
- interferon and immune responses
- Genomics and Chromatin Dynamics
- Plant-Microbe Interactions and Immunity
James Hutton Institute
2017-2021
Third Affiliated Hospital of Southern Medical University
2013-2021
University of Dundee
2019
First Hospital of Jilin University
2016
Jilin University
2016
We investigated whether the presence of Y chromosome azoospermia factor (AZF) microdeletions impacts upon outcomes intracytoplasmic sperm injection (ICSI) using fresh ejaculated spermatozoa. Sixteen oligozoospermia patients with AZFb or AZFc and undergoing ICSI cycles between March 2013 November 2014 were studied. Twenty-six infertile men normal chromosomes also IVF/ICSI in same time period used as controls. A retrospective case-control study approach was used. Among 16 cases, 12 (75%,...
Abstract RNA-seq analysis of gene expression and alternative splicing should be routine robust but is often a bottleneck for biologists because reliance on specialized bioinformatics skills. Thus, we have developed “ 3D ”, an R shiny App web based service which provides easy-to-use, flexible powerful tool three-component data: Differential Expression, Alternative Splicing Transcript Usage. integrates state-of-the-art, highly rated differential tools adopts best practice analysis. It operates...
Activation of cell-surface and intracellular receptor-mediated immunity results in rapid transcriptional reprogramming that underpins disease resistance. However, the mechanisms by which co-activation both immune systems lead to changes are not clear. Here, we combine RNA-seq ATAC-seq define gene expression chromatin accessibility. or immunity, both, increases accessibility at induced defence genes. Analysis data combined with publicly available information on transcription factor...
ABSTRACT Protein translation programs often select the longest open reading frame (ORF) in a transcript leading to numerous inaccurate and mis-annotated ORFs databases. Unproductive isoforms containing premature termination codons (PTCs) are potential substrates for nonsense-mediated decay (NMD). These transcripts contain truncated but incorrectly annotated due selection of long ORF beginning at an AUG downstream PTC despite authentic start AUG. In gene expression alternative splicing...
Co-expression has been widely used to identify novel regulatory relationships using high throughput measurements, such as microarray and RNA-seq data. Evaluation studies on co-expression network analysis methods mostly focus networks of small or medium size up a few hundred nodes. For large networks, simulated expression data usually consist hundreds thousands profiles with different perturbations knock-outs, which is uncommon in real experiments due their cost the amount work required....
Non-obstructive azoospermia (NOA) is among the most severe factors for male infertility, but our understandings of latent biological mechanisms remain insufficient. The single-cell RNA sequencing (scRNA-seq) data 432 testicular cells isolated from patient with NOA was analyzed, and cell samples were grouped into 5 clusters. A sum 455 markers identified then included in protein-protein interaction network. Top critical genes network, including CCT8, CDC6, PSMD1, RPS4X, RPL36A, selected...
Experimental animalsIn this study, 44 Meishan pigs (a native Chinese breed), 36 Large white pigs, 66 Line DIV 127 white× resource family F 2 offspring were bred and raised at the genetic nucleus station owned by Huazhong Agricultural University.Twenty-one Tongcheng 30 Erhualian derived from (Tongcheng, Hubei Province) Houqiao (Xishan, Jiangshu research farms in China, respectively.The purebred Qingping animals used study born farm of province China.At farrowing, total number (TNB) piglets...
Abstract Hyaloperonospora arabidopsidis ( Hpa ) is an oomycete pathogen causing Arabidopsis downy mildew. Effector proteins secreted from the into plant play key roles in promoting infection by suppressing immunity and manipulating host to pathogen’s advantage. One class of effectors share a conserved ‘RxLR’ motif critical for their translocation cell. Here we characterize interaction between RxLR effector, HaRxL21 (RxL21), transcriptional co-repressor Topless (TPL). We establish that RxL21...
● GERMIN3 has previously been identified as a target of the tuberigen activation complex suggesting function in potato tuberisation but its role is presently unknown. To understand we analysed morphological, agronomic and molecular phenotypes transgenic lines. over-expressing lines Solanum tuberosum ssp. andigena exhibited increased tuber yields under permissive conditions enhanced numbers. Post-harvest sprouting reduced apical dominance with numbers sprouts. Apical was aerial tissues mature...
Abstract Nonsense mediated RNA decay (NMD) is an evolutionary conserved control mechanism that has also been implicated in the broader regulation of gene expression. Nevertheless, a role for NMD genome not fully assessed, partially because inactivation lethal many organisms. Here, we performed depth comparative analysis Arabidopsis mutants lacking key proteins involved different steps NMD. We observed UPF3, UPF1, and SMG7 have impacts on transcriptome, with UPF1 having biggest effect....
A disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1motif (ADAMTS1) plays a critical role in follicular rupture represents major advance the proteolytic events that control ovulation. In this study, 9,026-bp DNA sequence containing full coding region, all 8 introns part of 5'and 3' untranslated region porcine ADAMTSI gene was obtained. Analysis using radiation hybrid panel indicated pig is closely linkage microsatellite marker S0215, located on SSC13q49. The...
Objective To indentify effects of the replication-deficient recombinant adenovirus carrying cytochrome P450 (CYP2B6) gene on human prostate cancer cell line PC-3.Methods The PC-3 cells were infected with CYP2B6 by MOI 200 VP/cell.The expression level mRNA and protein was detected using real-time quantitative polymerase chain reaction (Real-time PCR) Western blotting respectively.Results Compared to negative control,after infection gene,PC-3 expressed higher (8.12 ± 2.41 vs.0.92 ±0.19,P...
Abstract Background Testicular Ischemia reperfusion injury(IRI) is a major pathophysiological process of surgical reduction after testicular torsion, and oxidative stress the main injury factor. However, role BMSCs-derived exosomes in IRI its mechanism have not been reported. In this study, we investigated protective effect bone marrow mesenchymal stem cell-derived against ischemia-reperfusion injury. Methods Results: BMSCs were successfully isolated cultured from rat marrow, secreted by...
Abstract Background: Testicular Ischemia reperfusion injury(IRI) is a major pathophysiological process of surgical reduction after testicular torsion, and oxidative stress the main injury factor. However, role BMSCs-derived exosomes in IRI its mechanism have not been reported. In this study, we investigated protective effect bone marrow mesenchymal stem cell-derived against ischemia-reperfusion injury. Results : BMSCs were successfully isolated cultured from rat marrow, secreted by...