A5017553414- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Plant tissue culture and regeneration
- Plant Reproductive Biology
- Microtubule and mitosis dynamics
- DNA Repair Mechanisms
- Plant Genetic and Mutation Studies
- Plant Gene Expression Analysis
- Light effects on plants
- Plant-Microbe Interactions and Immunity
- Retinoids in leukemia and cellular processes
- Melanoma and MAPK Pathways
- Cancer-related Molecular Pathways
- Genomics and Phylogenetic Studies
- Plant biochemistry and biosynthesis
- Protein Degradation and Inhibitors
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- Congenital heart defects research
- Genomics and Chromatin Dynamics
- Plant Physiology and Cultivation Studies
- Protist diversity and phylogeny
- Ocular Oncology and Treatments
- Transgenic Plants and Applications
RIKEN Center for Sustainable Resource Science
2015-2022
Centre National de la Recherche Scientifique
2009-2016
Institut de Biologie Moléculaire des Plantes
2009-2016
Université de Strasbourg
2009-2016
Institut National des Sciences Appliquées de Strasbourg
2012-2013
Osaka International University
2011
Osaka University
2011
Nara Institute of Science and Technology
2007
Many plant species display remarkable developmental plasticity and regenerate new organs after injury. Local signals produced by wounding are thought to trigger organ regeneration but molecular mechanisms underlying this control remain largely unknown. We previously identified an AP2/ERF transcription factor WOUND INDUCED DEDIFFERENTIATION1 (WIND1) as a central regulator of wound-induced cellular reprogramming in plants. In study, we demonstrate that WIND1 promotes callus formation shoot...
Article6 August 2016Open Access Transparent process The plant-specific CDKB1-CYCB1 complex mediates homologous recombination repair in Arabidopsis Annika K Weimer Department of Molecular Mechanisms Phenotypic Plasticity, Institut de Biologie Moléculaire des Plantes du CNRS, IBMP-CNRS UPR2357, Université Strasbourg, Strasbourg Cedex, France Search for more papers by this author Sascha Biedermann Hirofumi Harashima orcid.org/0000-0003-3370-4111 RIKEN Center Sustainable Resource Science,...
The decision to replicate its DNA is of crucial importance for every cell and, in many organisms, decisive the progression through entire cycle. A comparison animals versus yeast has shown that, although most involved cell-cycle regulators are divergent both clades, they fulfill a similar role and overall network topology G1/S regulation highly conserved. Using germline development as model system, we identified regulatory cascade controlling entry into S phase flowering plant Arabidopsis...
To produce seeds, flowering plants need to specify somatic cells undergo meiosis. Here, we reveal a regulatory cascade that controls the entry into meiosis starting with group of redundantly acting cyclin-dependent kinase (CDK) inhibitors KIP-RELATED PROTEIN (KRP) class. KRPs function by restricting CDKA;1-dependent inactivation Arabidopsis Retinoblastoma homolog RBR1. In rbr1 and krp triple mutants, designated meiocytes several mitotic divisions, resulting in formation supernumerary give...
Cell cycle control is modified at meiosis compared to mitosis, because two divisions follow a single DNA replication event. Cyclin-dependent kinases (CDKs) promote progression through both and central regulator of their activity the APC/C (Anaphase Promoting Complex/Cyclosome) that especially required for exit from mitosis. We have shown previously OSD1 involved in entry into I II Arabidopsis thaliana; however, molecular mechanism by which controls these transitions has remained unclear....
The best-characterized members of the plant-specific SIAMESE-RELATED (SMR) family cyclin-dependent kinase inhibitors regulate transition from mitotic cell cycle to endoreplication, also known as endoreduplication, an altered version in which DNA is replicated without division. Some other are implicated responses biotic and abiotic stresses. However, functions most SMRs remain unknown, specific complexes inhibited by unclear. Here, we demonstrate that a diverse group SMRs, including SMR...
Formative, also called asymmetric, cell divisions produce daughter cells with different identities. Like other divisions, formative rely first of all on the cycle machinery centrally acting cyclin-dependent kinases (CDKs) and their cyclin partners to control progression through cycle. However, it is still largely obscure how developmental cues are translated at cellular level promote asymmetric divisions. Here, we show that in shoot root flowering plant Arabidopsis thaliana controlled by a...
Abstract The retinoblastoma protein (Rb), which typically functions as a transcriptional repressor of E2F‐regulated genes, represents major control hub the cell cycle. Here, we show that loss Arabidopsis Rb homolog RETINOBLASTOMA ‐ RELATED 1 ( RBR 1) leads to death, especially upon exposure genotoxic drugs such environmental toxin aluminum. While death can be suppressed by reduced cell‐proliferation rates, rbr1 mutant cells exhibit elevated levels DNA lesions, indicating direct role in...
Spatiotemporal regulation of transcription is fine-tuned at multiple levels, including chromatin compaction. Polycomb Repressive Complex 2 (PRC2) catalyzes the trimethylation Histone 3 lysine 27 (H3K27me3), which hallmark a repressive state. Multiple PRC2 complexes have been reported in Arabidopsis thaliana to control expression genes involved developmental transitions and maintenance organ identity. Here, we show that member display complex spatiotemporal gene patterns function root...
Retinoblastoma (pRb) is a multifunctional regulator, which was likely present in the last common ancestor of all eukaryotes. The Arabidopsis pRb homolog RETINOBLASTOMA RELATED 1 (RBR1), similar to its animal counterparts, controls not only cell proliferation but also implicated developmental decisions, stress responses and maintenance genome integrity. Although most functions pRb-type proteins involve chromatin association, genome-wide understanding RBR1 binding sites still missing. Here, we...
Article26 September 2019Open Access Source DataTransparent process The Arabidopsis Cdk1/Cdk2 homolog CDKA;1 controls chromosome axis assembly during plant meiosis Chao Yang Department of Developmental Biology, University Hamburg, Germany Search for more papers by this author Kostika Sofroni orcid.org/0000-0001-8648-4648 Erik Wijnker Yuki Hamamura Lena Carstens Hirofumi Harashima RIKEN Center Sustainable Resource Science, Yokohama, Japan Sara Christina Stolze Max-Planck-Institute Plant...
Flowering plants contain a large number of cyclin families, each containing multiple members, most which have not been characterized to date.Here, we analyzed the role B1 subclass mitotic cyclins in cell cycle control during Arabidopsis development.While reveal CYCB1;5 be pseudogene, remaining four members were found expressed dividing cells.Mutant analyses showed complex pattern overlapping, developmentspecific requirements B1-type with CYCB1;2 playing central role.The double mutant cycb1;1...
Cell proliferation is an important determinant of plant growth and development. In addition, modulation cell-division rate mechanism plasticity key in adapting plants to environmental conditions. One the greatest challenges understanding cell cycle flowering large families CDKs cyclins that have potential form many different complexes. However, it largely unclear which complexes are active. there CDK- cyclin-related proteins whose biological role still unclear, i.e. whether they indeed...
Cell cycle control must be modified at meiosis to allow two divisions follow a single round of DNA replication, resulting in ploidy reduction. The mechanisms that ensure termination the end second and not first division are poorly understood. We show here Arabidopsis thaliana TDM1, which has been previously shown essential for meiotic termination, interacts directly with Anaphase-Promoting Complex. Further, mutations TDM1 conserved putative Cyclin-Dependant Kinase (CDK) phosphorylation site...
Significance Cyclin-dependent kinases are the main drivers of mitotic cell cycle. Here, we show that activity cell-cycle regulator in model plant Arabidopsis, CDKA;1, also governs one most important processes meiosis: formation meiotic cross-overs. We CDKA;1 especially affects major class cross-overs, known as I find lowering kinase leads to a progressive loss which cross-overs near chromosome ends last disappear. Conversely, an increase increases rate cross-over formation.
Summary Although A‐type cyclin‐dependent kinase A (CDKA) is required for plant cell division, our understanding of how CDKA activated before the onset commitment to division limited. Here we show that phosphorylation threonine 161 (T161) in activation its associated kinase. Western blot analysis revealed T161 increased greatly, parallel with p13 suc1 ‐associated activity, when stationary‐phase tobacco BY‐2 cells were subcultured into fresh medium. induced over‐expression a dominant‐negative...
As a consequence of the transient nature kinase-substrate interactions, detection kinase targets, although central for understanding many biological processes, has remained challenging. Here we present straightforward procedure that relies on comparison wild type with activation-loop mutants in interest by bimolecular complementation assays. proof functionality, identification and vivo confirmation substrates major cell-cycle Arabidopsis, revealing direct link between cell proliferation...
Modulation of protein activity by phosphorylation through kinases and subsequent de-phosphorylation phosphatases is one the most prominent cellular control mechanisms. Thus, identification kinase substrates pivotal for understanding many – if not all molecular biological processes. Equally, possibility to deliberately tune great value analyze process controlled a particular kinase. Here we have applied chemical genetic approach generated an analog-sensitive version CDKA;1, central cell-cycle...