A5012187265- Plant Reproductive Biology
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Plant and animal studies
- Plant nutrient uptake and metabolism
- Plant tissue culture and regeneration
- Protist diversity and phylogeny
- Chromosomal and Genetic Variations
- Microtubule and mitosis dynamics
- Algal biology and biofuel production
- Plant Stress Responses and Tolerance
- Mitochondrial Function and Pathology
- Insect and Arachnid Ecology and Behavior
- Advanced Fluorescence Microscopy Techniques
- Plant Parasitism and Resistance
- Enzyme Production and Characterization
- Microbial Metabolites in Food Biotechnology
- Plant Virus Research Studies
- Nematode management and characterization studies
- Genetics, Aging, and Longevity in Model Organisms
- Slime Mold and Myxomycetes Research
- CRISPR and Genetic Engineering
- Genomics and Phylogenetic Studies
- Reproductive Biology and Fertility
- Plant Diversity and Evolution
The University of Tokyo
2004-2025
Bunkyo University
2025
Nagoya University
2015-2024
Hayashibara (Japan)
2000-2024
Japan Lifeline (Japan)
2024
Tokyo University of Science
2000-2023
Tohoku University
2021
Japan Science and Technology Agency
2006-2017
Graduate School USA
2013-2014
Higashi Osaka City General Hospital
2008
Small, compact genomes of ultrasmall unicellular algae provide information on the basic and essential genes that support lives photosynthetic eukaryotes, including higher plants1,2. Here we report 16,520,305-base-pair sequence 20 chromosomes red alga Cyanidioschyzon merolae 10D as first complete algal genome. We identified 5,331 in total, which at least 86.3% were expressed. Unique characteristics this genomic structure include: a lack introns all but 26 genes; only three copies ribosomal...
Imaging techniques for visualizing and analyzing precise morphology gene expression patterns are essential understanding biological processes during development in all organisms. With the aid of chemical screening, we developed a clearing method using solutions, termed ClearSee, deep imaging plant tissues. ClearSee rapidly diminishes chlorophyll autofluorescence while maintaining fluorescent protein stability. By adjusting refractive index mismatch, whole-organ whole-plant can be performed...
In flowering plants, guidance of the pollen tube to embryo sac (the haploid female gametophyte) is critical for successful fertilization. The target may attract as final step in pistil. We show by laser cell ablation that two synergid cells adjacent egg tube. A single was sufficient generate an attraction signal, and enhanced it. After fertilization, no longer attracts tube, despite persistence one cell. This cessation might be involved blocking polyspermy.
The circadian clock is an endogenous time-keeping mechanism that enables organisms to adapt external daily cycles. coordinates biological activities with these cycles, mainly through genome-wide gene expression. However, the exact underlying regulation of expression poorly understood. Here we demonstrated Arabidopsis PSEUDO-RESPONSE REGULATOR 5 (PRR5), which acts in genetic circuit, directly regulates timing key transcription factors involved clock-output pathways. A transient assay and...
Genes directly involved in male/female and host/parasite interactions are believed to be under positive selection. The flowering plant Arabidopsis thaliana has more than 300 defensin-like (DEFL) genes, which likely both natural immunity cell-to-cell communication including pollen–pistil interactions. However, little is known of the relationship between molecular evolution DEFL genes their functions. Here, we identified a recently evolved cluster A. demonstrated that these (cysteine-rich...
In flowering plants, pollen germinates on the stigma and tubes grow through style to fertilize ovules. Enzymatic production of reactive oxygen species (ROS) has been suggested be involved in tube tip growth. Here, we characterized function regulation NADPH oxidases RbohH RbohJ (Respiratory burst oxidase homolog H J) Arabidopsis thaliana. rbohH rbohJ single mutants, growth was comparable that wild type; however, severely impaired double mutant. vivo imaging showed ROS accumulation Both RbohJ,...
Significance Grain weight is an important crop yield component; however, its underlying regulatory mechanisms are largely unknown. Here, we identify a grain-weight quantitative trait locus (QTL) in rice encoding new-type GNAT-like protein that harbors intrinsic histone acetyltransferase activity (OsglHAT1). Elevated OsglHAT1 expression enhances grain and by enlarging spikelet hulls via increasing cell number accelerating filling, increases global acetylation levels of H4. Our findings reveal...
The circadian clock is a biological timekeeping system that provides organisms with the ability to adapt day-night cycles. Timing of expression four members Arabidopsis thaliana PSEUDO-RESPONSE REGULATOR (PRR) family crucial for proper function, and transcriptional control PRRs remains incompletely defined. Here, we demonstrate direct regulation PRR5 by CIRCADIAN CLOCK-ASSOCIATED1 (CCA1) determines repression state in morning. Chromatin immunoprecipitation followed deep sequencing (ChIP-seq)...
The CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated 9) system is widely used as a tool for genome engineering in various organisms. A complex consisting of Cas9 and single guide RNA (sgRNA) induces DNA double-strand break sequence-specific manner, resulting knockout. Some binary vectors plants have been reported, but there problem with low efficiency. Here, we present newly developed, highly efficient vector Arabidopsis thaliana, pKAMA-ITACHI Red...
Abstract Electron‐donating aryl groups were attached to electron‐accepting benzophosphole skeletons. Among several derivatives thus prepared, one oxide was particularly interesting, as it retained high fluorescence quantum yields even in polar and protic solvents. This phosphole‐based compound exhibited a drastic color change of its spectrum function the solvent polarity, while absorption spectra remained virtually unchanged. Capitalizing on these features, this used stain adipocytes, which...
Plant grafting is conducted for fruit and vegetable propagation, whereby a piece of living tissue attached to another through cell-cell adhesion. However, graft compatibility limits combinations closely related species, the mechanism poorly understood. We found that Nicotiana capable adhesion with diverse range angiosperms. Comparative transcriptomic analyses on indicated subclade β-1,4-glucanases secreted into extracellular region facilitates cell wall reconstruction near interface....
We examined five nucleic acid binding fluorescent dyes, propidium iodide, SYBR Green I, YO-PRO-1, TOTO-3, and TO-PRO-3, for nuclear DNA staining, visualized by fluorescence laser confocal microscopy. The optimal concentration, co-staining of RNA, bleaching speeds were examined. I TO-PRO-3 almost preferentially stained the DNA, other dyes co-stained cytoplasmic RNA. RNAse treatment completely prevented RNA staining. In conventional microscopy, these can be used in combination with...
Abstract Trehalose is a disaccharide with important functional properties. Although these properties have been recognized for many years, trehalose was not produced on an industrial scale. Recently, novel enzymatic system the production of developed, and cost has dramatically reduced enough to permit widespread application. In present study, stabilization mechanism unsaturated fatty acids by found. Furthermore, possible suppressive effects osteoporosis development emerged from our...
Chloroplasts have retained the bacterial FtsZ for division, whereas mitochondria lack except in some lower eukaryotes. Instead, mitochondrial division involves a dynamin-related protein, suggesting that chloroplasts system, dynamin-based system replaced during evolution. In this study, we identified novel plant-specific group of dynamins from primitive red alga Cyanidioschyzon merolae. Synchronization chloroplast and immunoblot analyses showed protein (CmDnm2) associates with only division....
The precise guidance of the pollen tube to embryo sac is critical successful sexual reproduction flowering plants. We demonstrate here in vitro by using naked Torenia fournieri, which protrudes from micropyle ovule. developed a medium for culture both ovule and T. fournieri cocultivated them thin layer solid medium. Although tubes that had germinated passed sacs, some grew semi-in through cut style arrived precisely at site entry into sac, namely, filiform apparatus synergids. When were...
Significance In animals and plants, the zygote divides unequally, daughter cells inherit different developmental fates to form a proper embryo along body axis. The cytological events leading polarization have remained unknown in flowering plants. Here, we report that two essential components of cytoskeleton, microtubules actin filaments, are both disorganized on fertilization then, arranged transverse ring directional cell elongation longitudinal arrays underlying polar nuclear migration,...