A5028132207- Plant tissue culture and regeneration
- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Plant Disease Management Techniques
- Plant Reproductive Biology
- Transgenic Plants and Applications
- Light effects on plants
- Plant Gene Expression Analysis
- Nematode management and characterization studies
- Plant-Microbe Interactions and Immunity
- Photosynthetic Processes and Mechanisms
- Plant Disease Resistance and Genetics
- Legume Nitrogen Fixing Symbiosis
- Cell Adhesion Molecules Research
- Glycosylation and Glycoproteins Research
- CRISPR and Genetic Engineering
- Plant Virus Research Studies
- Banana Cultivation and Research
- Plant Pathogens and Resistance
- Plant Stress Responses and Tolerance
- Chromosomal and Genetic Variations
- Plant Pathogens and Fungal Diseases
- Genetic Mapping and Diversity in Plants and Animals
- Angiogenesis and VEGF in Cancer
Nagoya University
2019-2025
Kyoto Sangyo University
2015-2022
Tokyo University of Science
2014
Plant grafting is conducted for fruit and vegetable propagation, whereby a piece of living tissue attached to another through cell-cell adhesion. However, graft compatibility limits combinations closely related species, the mechanism poorly understood. We found that Nicotiana capable adhesion with diverse range angiosperms. Comparative transcriptomic analyses on indicated subclade β-1,4-glucanases secreted into extracellular region facilitates cell wall reconstruction near interface....
Grafting techniques have been applied in studies of systemic, long-distance signaling several model plants. Seedling grafting Arabidopsis, known as micrografting, enables investigation the molecular mechanisms systemic between shoots and roots. However, conventional micrografting requires a high level skill, limiting its use. Thus, an easier user-friendly method is needed. Here, we developed silicone microscaled device, chip, to obviate need for training generate less stressed more uniformly...
Seasonal changes in spring induce flowering by expressing the florigen, FLOWERING LOCUS T (FT),
Seed formation is essential for plant propagation and food production. We present a novel mechanism the regulation of seed size by newly identified "gate" at chalazal end ovule regulating nutrient transport into developing seed. This gate blocked callose deposition in unfertilized mature ovules (closed state), but removed after central cell fertilization, allowing (open state). However, if fertilization fails, persists, preventing transportation nutrients from funiculus. A mutant an...
Grafting is an agricultural technique that joins tissues from different plants to obtain useful rootstock traits. However, cellular processes involved in joint tissue repair remain poorly understood. We analyzed Nicotiana benthamiana (Nb) and Arabidopsis thaliana (At) interfamily heterografting as a high-stress model At homografting low-stress model. Transmission electron micrographs reveal the formation of autophagic structures cells near graft boundary over long period Nb/At grafts short...
Plants are exposed to a variety of environmental stress, and starvation inorganic phosphorus can be major constraint in crop production. In plants, response phosphate deficiency soil, miR399, type microRNA (miRNA), is up-regulated. By detecting the early diagnosis stress plants accomplished. However, general miRNA detection methods require complicated experimental manipulations. Therefore, simple rapid required for plant nutritional diagnosis. For microfluidic technology suitable...
Grafting is an indispensable agricultural technology for propagating useful tree varieties and obtaining beneficial traits of two varieties/species—as stock scion—at the same time. Recent studies molecular events during grafting have revealed dynamic physiological transcriptomic changes. Strategies focused on specific steps are needed to further associate each event with those steps. In this study, we developed a method investigate tissue adhesion event, early step, by improving artificial...
Abstract Since ancient times, humans have bred several plants that we rely on today. However, little is known about the divergence of most these plants. In present study, investigated Mibuna ( Brassica rapa L. subsp. nipposinica H. Bailey), a traditional leafy vegetable in Kyoto (Japan), by combining genetic analysis and survey literature. considered to been 200 years ago from Mizuna, another Kyoto. has simple spatulate leaves, whereas Mizuna characteristic serrated leaves. The quantitative...
In order to maintain organs and structures at their appropriate sizes, multicellular organisms orchestrate cell proliferation post-mitotic expansion during morphogenesis. Recent studies using Arabidopsis leaves have shown that compensation, which is defined as induced by a decrease in the number of cells lateral organ development, one example such orchestration. Some basic molecular mechanisms underlying compensation been revealed genetic chimeric analyses. However, date, had observed only...
The tobacco BY-2 cell line has been used widely as a model system in plant biology. cells are nearly transparent, which facilitates imaging using fluorescent markers. As cultured drifted the medium, therefore, it was difficult to observe them for long period. Hence, we developed microfluidic device that traps and fixes their positions allow monitoring physiological activity of cells. contains 112 trap zones, with parallel slots connected series at three levels flow channel. were 7 days...
Summary Grafting techniques have been applied in studies of systemic, long-distance signaling several model plants. Seedling grafting Arabidopsis, known as micrografting, enables investigation the molecular mechanisms systemic between shoots and roots. However, conventional micrografting requires a high level skill, limiting its use. Thus, an easier user-friendly method is needed. Here, we developed silicone microscaled device, chip, to obviate need for training generate less stressed more...
Summary Grafting is an important technique in agriculture to obtain several good traits such as high disease tolerance and yield by exchanging root system. However, the underlined cellular processes compensate wound damage repair tissues were largely unknown. We analyzed two graft combinations: Nicotiana benthamiana ( Nb ) homograft a compatible, repairing model heterograft with Arabidopsis thaliana At incompatible more stressful model, which we recently identified exceptional maintainable...
Plant grafting is conducted for vegetative propagation in plants, whereby a piece of living tissue attached to another through establishment cell–cell adhesion. has long history agriculture and been applied improve crop traits thousands years 1 . mostly relied on the natural ability plant wound healing. However, compatibility adhesion typically limits graft combinations closely related species 2–4 , mechanism by which injured tissues established largely unknown. Here, we show that subclade...
Agrobacterium-mediated transformation is a key innovation for plant breeding, and routinely used in basic researches applied biology. However, the efficiency often limiting factor of this technique. In study, we discovered that oxicam-type nonsteroidal anti-inflammatory drugs, including tenoxicam (TNX), increase transient transformation. TNX treatment increased Arabidopsis thaliana mature leaves by agroinfiltration. The was not observed dde2/ein2/pad4/sid2 quadruple mutant, indicating...
Reactive oxygen species (ROS) are critical for plant biological processes. As signaling molecules, ROS regulate growth and development through cell expansion, elongation, programmed death. Furthermore, production is induced by microbe-associated molecular patterns (MAMPs) treatment biotic stresses, contributes to resistance pathogens. Thus, MAMP-induced has been an indicator early immune responses or stress responses. One of widely used methods the measurement a luminol-based assay measure...
The micrografting technique in the model plant Arabidopsis has been widely used field of science. Grafting experiments have demonstrated that signal transductions are systematically regulated many characteristics, including defense mechanisms and responses to surrounding environments such as soil light conditions. Hypocotyl is a powerful tool for analysis transduction between shoots roots; however, requirement high level skill micrografting, during which small seedlings microdissected...
Abstract Agrobacterium -mediated transformation represents a key innovation for plant breeding and is routinely used in research applied biology. However, several species, the efficacy of limited. In this study, we discovered that oxicam-type nonsteroidal anti-inflammatory drugs (NSAIDs), including tenoxicam (TNX), enhance efficiency transient model species Arabidopsis thaliana via leaf infiltration can be successfully analyses subcellular localisation fluorescent fusion proteins. TNX acts...
Abstract Plants are exposed to a variety of environmental stress and starvation inorganic phosphorus can be major constraint in crop production. In plants, response phosphate deficiency soil, miR399, type microRNA (miRNA), is upregulated. By detecting the early diagnosis plants accomplished. However, general miRNA detection methods require complicated experimental manipulations. Therefore, simple rapid required for plant nutritional diagnosis. For microfluidic technology suitable...