A5027349679- Particle physics theoretical and experimental studies
- High-Energy Particle Collisions Research
- Particle Detector Development and Performance
- Quantum Chromodynamics and Particle Interactions
- CRISPR and Genetic Engineering
- Plant tissue culture and regeneration
- Aerodynamics and Acoustics in Jet Flows
- Fluid Dynamics and Turbulent Flows
- Chromosomal and Genetic Variations
- Heat Transfer Mechanisms
- Dark Matter and Cosmic Phenomena
- Computational Fluid Dynamics and Aerodynamics
- Engineering Applied Research
- Plant Virus Research Studies
- Cosmology and Gravitation Theories
- Plant Genetic and Mutation Studies
- Plasma and Flow Control in Aerodynamics
- Magnetic properties of thin films
- DNA Repair Mechanisms
- Human Mobility and Location-Based Analysis
- Transgenic Plants and Applications
- Flow Measurement and Analysis
- Plant nutrient uptake and metabolism
- ZnO doping and properties
- Computational Physics and Python Applications
National Agriculture and Food Research Organization
2016-2024
Institute of Agrobiological Sciences
2015-2024
Ibaraki University
2011-2024
Tokyo Denki University
2006-2023
Yokohama City University
2015-2023
Kihara Institute for Biological Research
2015-2023
Tokyo Metropolitan University
1999-2022
Polytechnic University
2017-2022
The University of Tokyo
1998-2022
Osaka University
2010-2022
We have investigated the effect of applied electric field EG on thickness dependent magnetic anisotropy sputtered Co40Fe40B20 sandwiched with MgO and Ta. The range CoFeB explored is 2 nm below. As reduced, easy axis magnetization becomes perpendicular from in-plane. show that in-plane samples coercivity can be modified by applying at room temperature. Furthermore, superparamagnetic behavior observed for layers further reduced below ≈0.9 nm, where electric-field also their blocking
The CRISPR/Cas9 system is an efficient tool used for genome editing in a variety of organisms. Despite several recent reports successful targeted mutagenesis using the plants, each case target gene interest, Cas9 expression and guide-RNA (gRNA) used, tissues transformation subsequent differed, hence reported frequencies cannot be compared directly. Here, we evaluated mutation frequency rice different and/or gRNA cassettes under standardized experimental conditions. We introduced separately...
The clustered regularly interspaced short palindromic repeat (CRISPR)-associated endonuclease 9 (CRISPR/Cas9) system has been demonstrated to be a robust genome engineering tool in variety of organisms including plants. However, it shown that the CRISPR/Cas9 cleaves genomic DNA sequences containing mismatches guide RNA strand. We expected this low specificity could exploited induce multihomeologous and multiparalogous gene knockouts. In case polyploid plants, simultaneous modification...
Sequence-specific nucleases (SSNs) have been used successfully in homology-directed repair (HDR)-mediated gene targeting (GT) many organisms. However, break-induced GT plants remains challenging due to inefficient delivery of HDR templates and SSNs into plant nuclei. In plants, including rice, Agrobacterium-mediated transformation is the most practical means because this biotic system can deliver longer more intact DNA payloads with less incorporation fragmented compared physical systems...
RNA-guided genome editing using the CRISPR/Cas9 CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 (CRISPR-associated protein 9) system has been applied successfully in several plant species. However, to date, there are few reports on use of any current approaches grape—an important fruit crop with a large market not only for table grapes but also wine. Here, we report successful targeted mutagenesis grape (Vitis vinifera L., cv. Neo Muscat) system. When Cas9 expression...
Significance Genetically improving the root system architectures of plants is an effective strategy for developing climate-resilient crops. In this study, we revealed that a cloned rice quantitative trait locus associated with growth angle, qSOR1 , DRO1 homolog involved in gravitropic responses. The loss-of-function allele qsor1 resulted roots developed on soil surface and enabled to avoid reducing stress found saline paddy soils and, consequently, increased yields. We show homologs could be...
Elucidation of the genetic control rice architecture is crucial due to global demand for high crop yields. Rice a complex trait affected by plant height, tillering, and panicle morphology. In this study, principal component analysis (PCA) on 8 typical traits related revealed that first (PC), PC1, provided most information determine architecture. A genome-wide association study (GWAS) using PC1 as dependent variable was used isolate gene encoding rice, SPINDLY (OsSPY), activates gibberellin...
Temperature-dependent Sellmeier coefficients are necessary to optimize optical design parameters of the fiber transmission system. These calculated for fused silica (SiO/sub 2/), aluminosilicate, and Vycor glasses first time find temperature dependence chromatic dispersion at any wavelength from UV 1.7 /spl mu/m. The zero lambda//sub 0/ (1.273 mu/m SiO/sub 2/, 1.393 1.265 26/spl deg/C) varies linearly with temperature, d/spl 0//dT is 0.03 nm/K aluminosilicate glasses, whereas 2/ it 0.025...
Previous studies have demonstrated multiple herbicide resistance in California populations of Echinochloa phyllopogon, a noxious weed rice (Oryza sativa) fields. It was suggested that the to two classes acetolactate synthase-inhibiting herbicides, bensulfuron-methyl (BSM) and penoxsulam (PX), may be caused by enhanced activities herbicide-metabolizing cytochrome P450. We investigated BSM metabolism resistant (R) susceptible (S) lines E. which were originally collected from different areas...
CRISPR/Cas9 technology is a versatile tool for targeted mutagenesis in many organisms, including plants. However, this technique has not been applied to the Japanese morning glory (Ipomoea [Pharbitis] nil), traditional garden plant chosen National BioResource Project Japan. We selected dihydroflavonol-4-reductase-B (DFR-B) of I. nil, encoding an anthocyanin biosynthesis enzyme, as target gene, and changes stem colour were observed during early stages tissue culture by Rhizobium...
Summary Californian populations of Echinochloa phyllopogon have evolved multiple‐herbicide resistance ( MHR ), posing a threat to rice production in California. Previously, we identified two CYP 81A cytochrome P450 genes whose overexpression is associated with acetolactate synthase ALS ) inhibitors from chemical groups. Resistance mechanisms other herbicides remain unknown. We analyzed the sensitivity an line acetyl‐CoA carboxylase ACC ase) three groups, followed by analysis herbicide...
Common wheat has three sets of sub-genomes, making mutations difficult to observe, especially for traits controlled by recessive genes. Here, we produced hexaploid lines with loss function homeoalleles Qsd1, which controls seed dormancy in barley, Agrobacterium-mediated CRISPR/Cas9. Of the eight transformed events produced, independent carrying multiple Qsd1 were obtained. Notably, one line had every homeoallele. We crossed this plant wild-type cultivar Fielder generate a transgene-free...
Abstract The CRISPR/Cas9 system is an efficient and convenient tool for genome editing in plants. Cas9 nuclease derived from Streptococcus pyogenes (Sp ) commonly used this system. Recently, Staphylococcus aureus (SaCas9)-mediated was reported human cells Arabidopsis . Because SaCas9 (1053 a.a.) smaller than SpCas9 (1368 a.a.), could have substantial advantages delivering expressing protein, especially using virus vectors. Since the protospacer adjacent motif (PAM) sequence of (5′-NNGRRT-3′)...
In this study, we investigated intermediate-heat treatment (IHT) at temperatures between those of sintering and solution treatment, evaluated the effects on macrostructure, microstructure, magnetic properties Sm(Cobal.Fe0.35Cu0.06Zr0.018)7.8. We found that squareness was clearly improved by adopting IHT, which promotes grain growth. These results indicate reducing fraction boundaries increasing size affects behavior domain-wall motion. Magnetic Mr 1.22 T, HcJ 1580 kA/m, (BH)max 282 kJ/m3...
Recent reports of CRISPR- (clustered regularly interspaced short palindromic repeats)/Cas9 (CRISPR-associated protein 9) mediated heritable mutagenesis in plants highlight the need for accuracy directed by this system. Off-target mutations are an important issue when considering functional gene analysis, as well molecular breeding crop with large genome size, i.e. many duplicated genes, and where whole-genome sequence is still lacking. In mammals, off-target can be suppressed using Cas9...
Abstract Reproduction-specific small RNAs are vital regulators of germline development in animals and plants. MicroRNA2118 (miR2118) is conserved plants induces the production phased interfering (phasiRNAs). To reveal biological functions miR2118, we describe here rice mutants with large deletions miR2118 cluster. Our results demonstrate that loss causes severe male female sterility rice, associated marked morphological developmental abnormalities somatic anther wall cells. Small RNA...
NDRG1 and KAI1 belong to metastasis suppressor genes, which impede the dissemination of tumor cells from primary tumors distant organs. Previously, we identified promoting transcription factor, ATF3, as a downstream target NDRG1. Further analysis revealed that promoter contained consensus binding motif suggesting possibility suppresses through inhibition ATF3 expression followed by activation gene. In this report, found ectopic was able augment endogenous gene in prostate cancer cell lines,...
We investigated the voltage-controlled magnetic anisotropy (VCMA) in an ultrathin Ir-doped Fe layer with a CoxFe1−x termination layer. The VCMA effect depends on concentration of alloy, and large coefficient, as high −350 fJ/Vm, was obtained Co-rich First principles calculations revealed that increased is due not only to added Co atoms but also Ir adjacent atoms. Interface engineering using CoFe effective for recovering tunneling magnetoresistance while maintaining effect. developed...