A5024944049- Horticultural and Viticultural Research
- Fermentation and Sensory Analysis
- Plant Gene Expression Analysis
- Plant Physiology and Cultivation Studies
- Plant biochemistry and biosynthesis
- Plant Reproductive Biology
- Plant Pathogens and Fungal Diseases
- Postharvest Quality and Shelf Life Management
- Plant Molecular Biology Research
- Phytochemicals and Antioxidant Activities
- Light effects on plants
- CRISPR and Genetic Engineering
- Food Quality and Safety Studies
- Plant tissue culture and regeneration
- Flowering Plant Growth and Cultivation
- Plant nutrient uptake and metabolism
- Forest Insect Ecology and Management
- melanin and skin pigmentation
- Plant and animal studies
- Insect behavior and control techniques
- Plant Virus Research Studies
- Botanical Research and Applications
- Plant Disease Resistance and Genetics
- Photosynthetic Processes and Mechanisms
- Wine Industry and Tourism
National Agriculture and Food Research Organization
2011-2024
Institute of Fruit Tree and Tea Science
2015-2024
RNA-guided genome editing using the CRISPR/Cas9 CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 (CRISPR-associated protein 9) system has been applied successfully in several plant species. However, to date, there are few reports on use of any current approaches grape—an important fruit crop with a large market not only for table grapes but also wine. Here, we report successful targeted mutagenesis grape (Vitis vinifera L., cv. Neo Muscat) system. When Cas9 expression...
Grapevine (Vitis vinifera L.) is a species well known for its adaptation to radiation. However, photomorphogenic factors related UV-B responses have not been molecularly characterized. We cloned and studied the role of RECEPTOR (UVR1), ELONGATED HYPOCOTYL 5 (HY5), HY5 HOMOLOGUE (HYH) from V. vinifera. performed gene functional characterizations, generated co-expression networks, tested them in different environmental conditions. These genes complemented Arabidopsis uvr8 hy5 mutants...
A white-wine grape, Pinot Blanc, is thought to be a white-skinned mutant of red-wine Noir. Noir was heterozygous for VvmybA1. One allele the non-functional VvmybA1a, and other functional VvmybA1c. In however, only VvmybA1a observed, amount VvmybA1 DNA in Blanc half that These findings suggest deletion VvmybA1c from resulted Blanc.
Because of the commercial importance grapes (Vitis spp.), it is important to understand how grape coloration affected by genetic and environmental factors, as this knowledge may contribute more stable production high-quality grapes. The color berry skins determined mainly quantity composition anthocyanins. This review summarizes results recent studies regulation anthocyanin biosynthesis in skin: (i) myeloblastosis (MYB) haplotype at locus major factor that determines content. (ii) MYB...
The first genome sequence of an interspecific grape hybrid (Vitis labruscana × Vitis vinifera), 'Shine Muscat', elite table cultivar bred in Japan, is presented. resultant assemblies included two types sequences: a haplotype-phased the highly heterozygous genomes and unphased representing 'pseudo-haploid' genome. sequences, assembled to chromosome level with Hi-C reads, spanned 488.97 Mb length, 99.1% estimated size, 4,595 scaffold sequences 23.9-Mb N50 length. phased had 15,650 scaffolds...
Introduction: The skin color of grapes is deter-mined by the quantity and composition anthocy-anins. Black red cultivars accumulate anthocyanins in their skins, whereas white do not synthesize them. In grape, Myb-related genes such as VlmybA1-1 Vitis labruscana regulate anthocyanin biosynthesis controlling expression gene for UDP-glu-cose: flavonoid 3-O-glucosyltransferase (K
Flavonoid biosynthesis in grape (Vitis spp.) berry skin is affected by environmental factors such as light and temperature. However, the components of signaling low-temperature-induced abscisic acid (ABA) networks related to flavonoid accumulation have not been fully elucidated, how conditions affect these remains unclear. To clarify details possible light- ABA-related signal transduction networks, we developed a oligo-DNA microarray (38,549 independent probes) using publicly available...
A cDNA, VlmybA1-2, for an anthocyanin regulatory gene of grape was constructed under the control cauliflower mosaic virus (CaMV) 35SRNA promoter and introduced into leaf disks petioles kiwifruit (Actinidia deliciosa) plants by Agrobacterium-mediated transfer. When tissues were cultured on selection medium, red cells visible to naked eye formed at cut ends segments after approximately 2 weeks. The developed calluses, adventitious buds, reddish-purple plantlets 2, 3, 6 months culture,...
Transposition of transposable elements affect expression levels, splicing and epigenetic status, function genes located in, or near, the inserted/excised locus. For example, in grape, presence Gret1 retrotransposon promoter region VvMYBA1a allele at VvMYBA1 locus suppress transcription factor gene for anthocyanin biosynthesis this transposon insertion is responsible green berry skin color Vitis labrascana , ‘Shine Muscat’, a major grape cultivar Japan. To prove that transposons genome can be...
Effects of interstocks on the growth and fruit quality ‘Fuyu’ Japanese persimmon (Diospyros kaki Thunb.) were investigated to select an effective dwarfing interstock this species. The performance four (Ac-1, Ac-2, Y clones, a seedling Diospyros rhombifolia) was evaluated in combination with ‘Aogaki’ (D. kaki) rootstocks scions over six-year period. Ac-1 moderately reduced tree size without influencing yield efficiency (yield per unit crown area). In contrast, Ac-2 D. rhombifolia both...