A5031292223- Horticultural and Viticultural Research
- Plant biochemistry and biosynthesis
- Plant Gene Expression Analysis
- Genetic and phenotypic traits in livestock
- Plant Pathogens and Fungal Diseases
- Pancreatic and Hepatic Oncology Research
- Epigenetics and DNA Methylation
- Genomics and Phylogenetic Studies
- Invertebrate Immune Response Mechanisms
- Powdery Mildew Fungal Diseases
- Livestock and Poultry Management
- Immune Cell Function and Interaction
- Gene expression and cancer classification
- Genetic Neurodegenerative Diseases
- Scientific Computing and Data Management
- Bioinformatics and Genomic Networks
- Cancer Immunotherapy and Biomarkers
- Livestock Farming and Management
- Amyotrophic Lateral Sclerosis Research
- T-cell and B-cell Immunology
- Renal and related cancers
- Cholinesterase and Neurodegenerative Diseases
- Cancer-related gene regulation
- Glioma Diagnosis and Treatment
- Immunotherapy and Immune Responses
University of Tübingen
2016-2024
University Children's Hospital Tübingen
2024
Universitätsklinikum Tübingen
2023
Quantitative Biology Center
2016-2022
Heidelberg University
2012-2017
Hertie Institute for Clinical Brain Research
2016
University of Nevada, Reno
2013-2015
Bielefeld University
2009
Plant Industry
2009
Cooperative Research Centre for Tissue Growth and Repair
2009
Abstract Flavonols are important ultraviolet light protectants in many plants and contribute substantially to the quality health-promoting effects of fruits derived plant products. To study regulation flavonol synthesis fruit, we isolated characterized grapevine (Vitis vinifera ‘Shiraz’) R2R3-MYB transcription factor VvMYBF1. Transient reporter assays established VvMYBF1 be a specific activator synthase1 (VvFLS1) several other promoters Arabidopsis (Arabidopsis thaliana) genes involved...
Plant stilbenes are phytoalexins that accumulate in a small number of plant species, including grapevine (Vitis vinifera), response to biotic and abiotic stresses have been implicated many beneficial effects on human health. In particular, resveratrol, the basic unit all other complex stilbenes, has received widespread attention because its cardio-protective, anticarcinogenic, antioxidant properties. Although stilbene synthases (STSs), key enzymes responsible for resveratrol biosynthesis,...
Although mutated HLA ligands are considered ideal cancer-specific immunotherapy targets, evidence for their presentation is lacking in hepatocellular carcinomas (HCCs). Employing a unique multi-omics approach comprising neoepitope identification pipeline, we assessed exome-derived mutations naturally presented as class I HCCs.
Grapevine (Vitis vinifera L.) is a species well known for its adaptation to radiation. However, photomorphogenic factors related UV-B responses have not been molecularly characterized. We cloned and studied the role of RECEPTOR (UVR1), ELONGATED HYPOCOTYL 5 (HY5), HY5 HOMOLOGUE (HYH) from V. vinifera. performed gene functional characterizations, generated co-expression networks, tested them in different environmental conditions. These genes complemented Arabidopsis uvr8 hy5 mutants...
BACKGROUND AND AIMS Programmed death 1 (PD‐1) checkpoint inhibition has shown promising results in patients with hepatocellular carcinoma, inducing objective responses approximately 20% of treated patients. The roles other coinhibitory molecules and their individual contributions to T‐cell dysfunction liver cancer, however, remain largely elusive. APPROACH RESULTS We performed a comprehensive mRNA profiling cluster differentiation 8 (CD8) T cells murine model autochthonous cancer by...
In grapevine, flavonoids constitute one of the most abundant subgroups secondary metabolites, influencing quality, health value, and typicity wines. Their synthesis in many plant species is mainly regulated at transcriptional level by modulation flavonoid pathway genes either single regulators or complexes different regulators. particular, bZIP MYB factors interact synergistically recognition light response units present promoter some pathway, thus mediating light-dependent biosynthesis. We...
Some pathogenic species of the Botryosphaeriaceae have a latent phase, colonizing woody tissues while perennial hosts show no apparent symptoms until conditions for disease development become favorable. Detection these pathogens is often limited to later phase. The phase poorly characterized, despite need non-destructive detection tools and effective quarantine strategies, which would benefit from identification host-based markers in leaves. Neofusicoccum parvum infects wood grapevines other...
European grapevine cultivars (Vitis vinifera spp.) are highly susceptible to the downy mildew pathogen Plasmopara viticola. Breeding of resistant V. is a promising strategy reduce impact disease management. Most that have been bred for resistance mildew, rely on mediated by Rpv3 (Resistance P. viticola) locus. However, despite extensive use this locus, little known about mechanism Rpv3-mediated resistance.In study, defense responses were investigated in Rpv3+ and Rpv3- following inoculation...
Flavonols constitute a group of flavonoids with important photoprotective roles in plants. In addition, flavonol content and composition greatly influences fruit quality. We previously demonstrated that the grapevine R2R3-MYB transcription factor (TF) VviMYBF1 promotes accumulation by inducing expression synthase (VviFLS1/VviFLS4), key step initial pathway. Despite this, gene networks underlying modification including both structural regulatory genes remain poorly understood. order to...
Background and aims Besides well-defined genetic alterations, the dedifferentiation of mature acinar cells is an important prerequisite for pancreatic carcinogenesis. Acinar-specific genes controlling cell homeostasis are extensively downregulated during cancer development; however, underlying mechanisms poorly understood. Now, we devised a novel in vitro strategy to determine genome-wide dynamics epigenetic landscape Design With our carcinogenic sequence, performed global gene expression...
Amyotrophic Lateral Sclerosis (ALS) is a fatal neurodegenerative disease mainly affecting upper and lower motoneurons. Several functionally heterogeneous genes have been associated with the familial form of this disorder (fALS), depicting an extremely complex pathogenic landscape. This heterogeneity has limited identification effective therapy, bleak prognosis will only improve greater understanding convergent mechanisms. Recent evidence from human post-mortem material diverse model systems...
Abstract Background and objective The discovery of cell-free micro-RNAs in body fluids has made them a promising biomarker target the field neurodegenerative diseases. Although they have been reported to be differentially expressed biofluids tissues from sporadic Parkinson’s disease patients, it remains unclear whether similar observations can patients with genetic forms if miRNA profiles reflect mutation-specific pathogenic pathways. Since induced pluripotent stem cell-derived neurons...
Modern biomedical research aims at drawing biological conclusions from large, highly complex datasets. It has become common practice to make extensive use of high-throughput technologies that produce big amounts heterogeneous data. In addition the ever-improving accuracy, methods are getting faster and cheaper, resulting in a steadily increasing need for scalable data management easily accessible means analysis. We present qPortal, platform providing users with an intuitive way manage...
Despite the widespread use of antiseptics such as chlorhexidine digluconate (CHX) in dental practice and oral care, risks potential resistance toward these antimicrobial compounds bacteria have only been highlighted very recently. Since molecular mechanisms behind antiseptic or adaptation are not entirely clear bacterial stress response has investigated systematically so far, aim present study was to investigate transcriptomic Streptococcus mutans after treatment with CHX using RNA...
Cell membrane-associated epidermal growth factor receptor (EGFR) translocates into a perinuclear/nuclear location upon stimulation, where it complexes with mRNAs. Treatment radiation and cisplatin decreases the amounts of mRNAs present within this complex. Gene array analyses in complex immunoprecipitated nEGFR revealed significant enrichment different mRNA species compared to control immunoprecipitation. Functional annotation help DAVID Ontology Analysis identified under other terms...
The induction of flower buds in apple ( Malus × domestica Borkh.) is tightly connected to biennial bearing, which characterized by alternating years with high (ON) and low or no (OFF) crop loads. In order study this irregular cropping behavior, spur from ON- OFF-trees the biennial-bearing cultivar ‘Fuji’ regular bearing ‘Gala’ were collected. First, time bud initiation was precisely determined for both cultivars histological analysis. Moreover, a systematic understanding apple, physiological...
The use of radiation treatment has increased for both sporadic and neurofibromatosis type 2 (NF2)-associated vestibular schwannoma (VS). However, there are a subset radioresistant tumors systemic treatments that seldom used in these patients. We investigated molecular alterations after three NF2-associated five sporadically operated recurrent VS primary irradiation. compared findings with 49 non-irradiated (36 13 NF2-associated) through gene-expression profiling pathway analysis....
African indigenous taurine cattle display unique adaptive traits shaped by husbandry management, regional climate and exposure to endemic pathogens. They are less productive with respect milk meat production which has been associated amongst others, small size, traditional beliefs, practices, limited feed resources, disease burden lack of sustained breeding for trait improvement. This resulted in the severe dwindling their population size rendering them vulnerable extinction. The Namchi...
Strain ATCC 31962 was formerly taxonomically classified as Empedobacter haloabium and reported to be the producer of lipopeptide antibiotic empedopeptin. Here, we report draft genome sequence 31962, which encodes regions that suggest a distinct biosynthetic capacity suggests its taxonomic reclassification.
Abstract Background The overexpression of (basic)helix-loop-helix ((b)HLH) transcription factors (TFs) is frequent in malignant glioma. We investigated molecular effects upon disruption the (b)HLH network by a dominant-negative variant E47 protein (dnE47). Our goal was to identify novel subgroup-specific therapeutic strategies. Methods Glioma cell lines LN229, LNZ308, and GS-2/GS-9 were lentivirally transduced. Functional characterization included immunocytochemistry, immunoblots, cytotoxic,...
Abstract Amyotrophic Lateral Sclerosis (ALS) is a fatal neurodegenerative disease mainly affecting upper and lower motoneurons. Several functionally heterogeneous genes have been associated with the familial form of this disorder (fALS), depicting an extremely complex pathogenic landscape. This heterogeneity has limited identification effective therapy, bleak prognosis will only improve greater understanding convergent mechanisms. Recent evidence from human post-mortem material diverse model...
Hepatocellular carcinoma (HCC) is the second leading cause of cancer-related death and fifth most common kind cancer worldwide. Anti-cancer immune responses are often hampered by upregulation co-inhibitory receptors on surface CD8 T-cells accompanied reduced T-cell functionality, described as T-cell-exhaustion. We have utilized Sleeping beauty" (SB)/transposon system for development an autochtonous HCC mouse model. Using adoptive transfer allowed us in-depth phenotyping tumor-specific we...