A5002001500- CRISPR and Genetic Engineering
- Plant tissue culture and regeneration
- Chromosomal and Genetic Variations
- Plant Virus Research Studies
- Transgenic Plants and Applications
- Plant Genetic and Mutation Studies
- Photosynthetic Processes and Mechanisms
- DNA Repair Mechanisms
- Plant Reproductive Biology
- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- GABA and Rice Research
- Plant-Microbe Interactions and Immunity
- Insect symbiosis and bacterial influences
- Plant Gene Expression Analysis
- Ubiquitin and proteasome pathways
- Fungal and yeast genetics research
- RNA and protein synthesis mechanisms
- Plant Stress Responses and Tolerance
- Insect Resistance and Genetics
- Animal Genetics and Reproduction
- Plant Micronutrient Interactions and Effects
- Genetically Modified Organisms Research
- Weed Control and Herbicide Applications
- Plant and animal studies
Institute of Agrobiological Sciences
2015-2024
Yokohama City University
2015-2024
Kihara Institute for Biological Research
2014-2024
National Agriculture and Food Research Organization
2013-2024
Ryukoku University
2021-2024
Tokushima University
2015
Agrogen (Czechia)
2013
RIKEN
2011
Chiba University
2011
The University of Tokyo
1994-2010
Summary Several approaches have recently been adopted to improve Agrobacterium ‐mediated transformation of rice, both generate the large number T‐DNA insertion plants needed for functional analysis rice genome, and production with additional agronomical value. However, about 3 months in vitro culture is still required isolation transgenic plants. Here, we report competency scutellum tissue from 1‐day pre‐cultured seeds transformation. Furthermore, early infection enhanced efficient selection...
The Xa1 gene in rice confers resistance to Japanese race 1 of Xanthomonas oryzae pv. , the causal pathogen bacterial blight (BB). We isolated by a map-based cloning strategy. deduced amino acid sequence product contains nucleotide binding sites (NBS) and new type leucine-rich repeats (LRR); thus, is member NBS-LRR class plant disease-resistance genes, but quite different from Xa21 another BB-resistance rice. Interestingly, expression was induced on inoculation with wound, unlike other genes...
Abstract Benzothiadiazole (BTH) is a so-called plant activator and protects plants from diseases by activating the salicylic acid (SA) signaling pathway. By microarray screening, we identified BTH- SA-inducible WRKY transcription factor (TF) genes that were upregulated within 3 h after BTH treatment. Overexpression of one them, WRKY45, in rice (Oryza sativa) markedly enhanced resistance to blast fungus. RNA interference–mediated knockdown WRKY45 compromised BTH-inducible disease, indicating...
Site-directed mutagenesis in higher plants remains a significant technical challenge for basic research and molecular breeding. Here, we demonstrate targeted-gene inactivation an endogenous gene Arabidopsis using zinc finger nucleases (ZFNs). Engineered ZFNs stress-response regulator, the ABA-INSENSITIVE4 ( ABI4 ) gene, cleaved their recognition sequences specifically vitro, ZFN genes driven by heat-shock promoter were introduced into genome. After induction, mutations with deletion...
Abstract CRISPR/Cas9 systems are nowadays applied extensively to effect genome editing in various organisms including plants. CRISPR from Prevotella and Francisella 1 (Cpf1) is a newly characterized RNA-guided endonuclease that has two distinct features as compared Cas9. First, Cpf1 utilizes thymidine-rich protospacer adjacent motif (PAM) while Cas9 prefers guanidine-rich PAM. could be used sequence-specific nuclease target AT-rich regions of had difficulty accessing. Second, generates DNA...
The CRISPR/Cas9 system is an efficient tool used for genome editing in a variety of organisms. Despite several recent reports successful targeted mutagenesis using the plants, each case target gene interest, Cas9 expression and guide-RNA (gRNA) used, tissues transformation subsequent differed, hence reported frequencies cannot be compared directly. Here, we evaluated mutation frequency rice different and/or gRNA cassettes under standardized experimental conditions. We introduced separately...
The clustered regularly interspaced short palindromic repeat (CRISPR)-associated endonuclease 9 (CRISPR/Cas9) system has been demonstrated to be a robust genome engineering tool in variety of organisms including plants. However, it shown that the CRISPR/Cas9 cleaves genomic DNA sequences containing mismatches guide RNA strand. We expected this low specificity could exploited induce multihomeologous and multiparalogous gene knockouts. In case polyploid plants, simultaneous modification...
Sequence-specific nucleases (SSNs) have been used successfully in homology-directed repair (HDR)-mediated gene targeting (GT) many organisms. However, break-induced GT plants remains challenging due to inefficient delivery of HDR templates and SSNs into plant nuclei. In plants, including rice, Agrobacterium-mediated transformation is the most practical means because this biotic system can deliver longer more intact DNA payloads with less incorporation fragmented compared physical systems...
RNA-guided genome editing using the CRISPR/Cas9 CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 (CRISPR-associated protein 9) system has been applied successfully in several plant species. However, to date, there are few reports on use of any current approaches grape—an important fruit crop with a large market not only for table grapes but also wine. Here, we report successful targeted mutagenesis grape (Vitis vinifera L., cv. Neo Muscat) system. When Cas9 expression...
The effects of wortmannin (WT), an inhibitor phosphatidylinositol (PI) 3-kinase, on differentiation PC12 cells were analyzed. WT inhibited PI 3-kinase activity at a concentration 10(-7) M in vivo and vitro. Transient inhibition the time nerve growth factor stimulation had no effect activation ras protein or neurite formation by cells. However, continuous blocked step just before formation. When was applied to growing neurites, elongation neurites stopped that step. These results suggest may...
Iron is essential for most living organisms, and thus iron deficiency poses a major abiotic stress in crop production. Plants induce utilization systems under conditions of low availability, but the molecular mechanisms gene regulation remain largely unknown. We identified novel transcription factor rice barley, IDEF2, which specifically binds to deficiency-responsive cis-acting element 2 (IDE2) by yeast one-hybrid screening. IDEF2 belongs an uncharacterized branch NAC family exhibits...
Previous studies have demonstrated multiple herbicide resistance in California populations of Echinochloa phyllopogon, a noxious weed rice (Oryza sativa) fields. It was suggested that the to two classes acetolactate synthase-inhibiting herbicides, bensulfuron-methyl (BSM) and penoxsulam (PX), may be caused by enhanced activities herbicide-metabolizing cytochrome P450. We investigated BSM metabolism resistant (R) susceptible (S) lines E. which were originally collected from different areas...
CRISPR/Cas9 technology is a versatile tool for targeted mutagenesis in many organisms, including plants. However, this technique has not been applied to the Japanese morning glory (Ipomoea [Pharbitis] nil), traditional garden plant chosen National BioResource Project Japan. We selected dihydroflavonol-4-reductase-B (DFR-B) of I. nil, encoding an anthocyanin biosynthesis enzyme, as target gene, and changes stem colour were observed during early stages tissue culture by Rhizobium...
Summary Californian populations of Echinochloa phyllopogon have evolved multiple‐herbicide resistance ( MHR ), posing a threat to rice production in California. Previously, we identified two CYP 81A cytochrome P450 genes whose overexpression is associated with acetolactate synthase ALS ) inhibitors from chemical groups. Resistance mechanisms other herbicides remain unknown. We analyzed the sensitivity an line acetyl‐CoA carboxylase ACC ase) three groups, followed by analysis herbicide...