A5015933283- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Molecular Sensors and Ion Detection
- Metal-Catalyzed Oxygenation Mechanisms
- Luminescence and Fluorescent Materials
- Metal complexes synthesis and properties
- Electron Spin Resonance Studies
- Click Chemistry and Applications
- Electrochemical Analysis and Applications
- Photosynthetic Processes and Mechanisms
- Microbial metabolism and enzyme function
- Nanoplatforms for cancer theranostics
- Advanced Fluorescence Microscopy Techniques
- Oxidative Organic Chemistry Reactions
- Crystallography and molecular interactions
- Advanced biosensing and bioanalysis techniques
- Magnetism in coordination complexes
- Lipid Membrane Structure and Behavior
- Lanthanide and Transition Metal Complexes
- Mitochondrial Function and Pathology
- Amino Acid Enzymes and Metabolism
- Radical Photochemical Reactions
- Synthesis and characterization of novel inorganic/organometallic compounds
- Porphyrin Metabolism and Disorders
- Trace Elements in Health
Nagoya University
2015-2024
Japan Science and Technology Agency
2017-2020
The University of Tokyo
1978-2020
Kawaguchi Junior College
2017
Kyoto University
2005-2014
Osaka University
1999-2011
Osaka Medical and Pharmaceutical University
2008-2011
Osaka Research Institute of Industrial Science and Technology
2011
SPring-8
2011
RIKEN
2011
Zinc and calcium are ubiquitous intracellular metals, while a variety of quantitative probes have been developed for measuring changes in concentration, the same is not true zinc. We describe here design, synthesis, properties benzoxazole-based, ratiometric zinc probe, Zinbo-5. This bright fluorescent reporter has quantum yield 0.1 zinc-form, exhibits Kd Zn2+ nanomolar range, shows significant both excitation emission maxima upon binding. The utility this cell permeable probe demonstrated...
Significance Stimulated emission depletion (STED) microscopy is one of the most appealing tools to visualize nanoscale cellular structures and dynamics in living cells. However, its practical utility significantly limited by rapid photobleaching fluorescent dyes under ultrastrong laser. In this context, superphotostable probes, which enable repeated recording STED images, are crucial chemical extend application live-cell imaging. Herein, we report a developed probe, MitoPB Yellow, has...
Abstract Electron‐donating aryl groups were attached to electron‐accepting benzophosphole skeletons. Among several derivatives thus prepared, one oxide was particularly interesting, as it retained high fluorescence quantum yields even in polar and protic solvents. This phosphole‐based compound exhibited a drastic color change of its spectrum function the solvent polarity, while absorption spectra remained virtually unchanged. Capitalizing on these features, this used stain adipocytes, which...
We describe here the new copper-selective fluorescent probes FluTPA1 and FluTPA2, in which tetradentate ligand tris[(2-pyridyl)methyl]amine (TPA) is connected to a reduced form of fluorescein platform through benzyl ether linkage. These selectively react with copper ions presence submillimolar glutathione emit intense green fluorescence from reaction product, O-methylfluorescein. Confocal images live cells further show that FluTPA2 membrane-permeable allows visualization CuI present living cells.
We described here a coumarin-based dual-excitation ratiometric probe for cadmium, CadMQ. This fluorescence sensor has high quantum yields of 0.59 and 0.70 in the metal-free Cd2+-bound forms, respectively, dissociation constant 0.16 nM Cd2+. CadMQ is cell permeable locates within acidic compartments cells. further show that useful tool to ratiometrically change intracellular Cd2+ levels with use two excited wavelengths.
As stimulated emission depletion (STED) microscopy can provide structural details of cells with an optical resolution beyond the diffraction limit, it has become indispensable tool in cell biology. However, intense STED laser beam usually causes rapid photobleaching employed fluorescent dyes, which significantly limits utility from a practical perspective. Herein we report new design super-photostable dye, PhoxBright 430 (PB430), comprising fully ring-fused π-conjugated skeleton...
Abstract NHC‐Au I complexes were used to prepare stable, water‐soluble, NHC‐protected gold nanoparticles. The charged nature of the nanoparticles permitted analysis by polyacrylamide gel electrophoresis (PAGE), which showed that highly monodisperse, with tunable core diameters between 2.0 and 3.3 nm depending on synthesis conditions. Temporal, thermal, chemical stability determined be high. Treatment thiols caused etching particles after 24 h; however larger plasmonic greater resistance...
Various fluorescence microscopy techniques require bright NIR-emitting fluorophores with high chemical and photostability. Now, the significant performance improvement of phosphorus-substituted rhodamine dyes (PORs) upon substitution at 9-position a 2,6-dimethoxyphenyl group is reported. The thus obtained dye PREX 710 was used to stain mitochondria in living cells, which allowed long-term three-color imaging vis-NIR range. Moreover, longevity allows tracking dye-labeled biomolecule by...
Abstract The development of stimulated emission depletion (STED) microscopy represented a major breakthrough in cellular and molecular biology. However, the intense laser beams required for both excitation STED usually provoke rapid photobleaching fluorescent probes, which significantly limits performance practical utility microscopy. We herein developed photoresistant dye C‐Naphox as tool imaging. With using either λ =405 or 488 nm protic solvents, exhibited an red/orange fluorescence...
Phospha-fluorescein (POF), a phosphine oxide-containing analogue of fluorescein, was synthesized and its photophysical properties were examined. Compared with fluorescein sila-fluorescein, POF displayed significantly red-shifted absorption fluorescence as well superior photobleaching resistance, while retaining the pH-responsive characteristics dyes.
A series of tetracationic quadrupolar chromophores containing three-coordinate boron π-acceptors linked by different π-bridges, namely 4,4'-biphenyl, 2,7-pyrene, 2,7-fluorene, 3,6-carbazole and 5,5'-di(thien-2-yl)-3,6-diketopyrrolopyrrole, were synthesized. While their neutral precursors 1-5 displayed highly solvatochromic fluorescence, the water-soluble target molecules 1M-5M, did not, but emission colour could be tuned from blue to pink changing π-bridge. Compound 5M, diketopyrrolopyrrole...
Abstract Metabolic distribution of fatty acid to organelles is an essential biological process for energy homeostasis as well the maintenance membrane integrity, and metabolic pathways are strictly regulated in response environmental stimuli. Herein, we report a fluorescent probe, which bears azapyrene dye that changes its absorption emission features depending on microenvironment polarity organelle into it transported. Owing sensitivity this dye, metabolically incorporated probe non-polar...
Abstract The inner mitochondrial membrane (IMM) undergoes dynamic morphological changes, which are crucial for the maintenance of functions as well cell survival. As dynamics governed by its lipid components, a fluorescent probe that can sense spatiotemporal alterations in properties IMM over long periods time is required to understand physiological detail. Herein, we report red‐emissive IMM‐labeling reagent with excellent photostability and sensitivity environment, enables visualization...
The first systematic studies on the oxidation of neutral phenols (ArOH) by mu-eta(2):eta(2)-peroxo)dicopper(II) complex (A) and bis(mu-oxo)dicopper(III) (B) supported 2-(2-pyridyl)ethylamine tridentate didentate ligands L(Py2) L(Py1), respectively, have been carried out in order to get insight into phenolic O-H bond activation mechanism metal-oxo species. In both cases (A B), C-C coupling dimer was obtained as a solely isolable product approximately 50% yield base dicopper-dioxygen...
By using molecular oxygen bis(µ-oxo)dicopper(III) complexes can be produced from Cu(I) with ligand L(X) (L(X)=p-substituted N-ethyl-N-[2-(2-pyridyl)ethyl]-2-phenylethylamine; X=OMe, Me, H, Cl, NO(2)) in which the benzylic position of is activated and hydroxylated by Cu(2)O(2) core (see reaction scheme). Detailed characterization this new C-H bond activation reveals important information on fundamental chemistry underlying copper monooxygenase reactivity.
The synthesis and photophysical properties of a rosamine-based fluorescent chemosensor, RosAg, for detecting Ag ion in an aqueous solution are described. This sensor has negligible quantum yield (<0.005) the absence Ag+, whereas significant increase fluorescence is observed upon complexation with Ag+ under physiological conditions. crystal structure silver complex chelator moiety RosAg reveals trigonal-planar coordination geometry which three S atoms occupy metal center. Although strong...
A Hg(2+)-selective fluorescent sensor, RosHg, has been developed based on a rosamine platform. RosHg exhibited ∼20-fold increase in fluorescence emission upon binding with Hg(2+), and the enhanced was immediately decreased when glutathione added to solution of Hg-RosHg complex. The dissociation constant for Hg(2+) complex determined be 0.10 fM by using set Hg(2+)/Mg(2+)/ethylenediaminetetraacetic acid buffer solutions. Confocal microscopy experiments demonstrated that this sensor can monitor...
Abstract Bright fluorescent molecules with long fluorescence lifetimes are important for the development of lifetime‐based imaging techniques. Herein, a molecular design is described simultaneously attaining lifetime ( τ ) and high brightness Φ F × ɛ in system that features macrocyclic dimerization π‐conjugated skeletons flexible linkers. An alkylene‐linked dimer bis(thienylethynyl)anthracene was found to show excimer emission ≈19 ns) solution, while maintaining brightness. A comparison...
Abstract Mitochondrial DNA (mtDNA) leakage into the cytoplasm can occur when cells are exposed to noxious stimuli. Specific sensors recognize cytoplasmic mtDNA promote cytokine production. Cytoplasmic also be secreted extracellularly, leading sterile inflammation. However, mode of secretion out upon stimuli and its relevance human disease remain unclear. Here, we show that pyroptotic secrete encapsulated within exosomes. Activation caspase‐1 leads from mitochondria via gasdermin‐D. Caspase‐1...
Copper(I)-dioxygen reactivity has been examined using a series of 2-(2-pyridyl)ethylamine bidentate ligands (R1)Py1(R2,R3). The ligand with the methyl substituent on pyridine nucleus (Me)Py1(Et,Bz) (N-benzyl-N-ethyl-2-(6-methylpyridin-2-yl)ethylamine) predominantly provided (mu-eta(2):eta(2)-peroxo)dicopper(II) complex, while without 6-methyl group (H)Py1(Et,Bz) (N-benzyl-N-ethyl-2-(2-pyridyl)ethylamine) afforded bis(mu-oxo)dicopper(III) complex under same experimental conditions. Both...
Two different chromophores, namely a dipolar and an octupolar system, were prepared their linear nonlinear optical properties as well bioimaging capabilities compared. Both contain triphenylamine the donor triarylborane acceptor, latter modified with cationic trimethylammonio groups to provide solubility in aqueous media. The system exhibits much higher two-photon brightness, also better cell viability enhanced selectivity for lysosomes compared chromophore. Furthermore, both dyes applied...
The stability of tetracationic triarylboranes in dilute aqueous solution was investigated by tuning the steric demand linker a (para-(N,N,N-trimethylammonio)xylyl)2 B-(linker)-B(para-(N,N,N-trimethylammonio)xylyl)2 structure. With increasing bulk linker, namely 1,4-phenylene, 2,2'''-(3,3'''-dimethyl)-5,2':5',2'':5'',5'''-quaterthiophene, 9,10-anthracenylene, and 4,4'''-(5'-(3,5-dimethylphenyl))(5''-(3''',5'''-dimethylphenyl))-2',2''-bithiophene, compounds increased. anthracene-based...
Super-resolution imaging techniques have become increasingly important tools to visualize suborganelle structures and dynamic processes in living cells on the nanoscale. However, utility of these is currently limited by availability advanced fluorescent probes that enable specific labeling organelle interest provide absorption/emission properties required for super-resolution techniques. Herein, LysoPB Yellow presented as a new small-molecule probe can selectivity stain lysosomal membrane....