A5068600093- Plant Molecular Biology Research
- Plant Reproductive Biology
- earthquake and tectonic studies
- Plant tissue culture and regeneration
- Plant Stress Responses and Tolerance
- Plant nutrient uptake and metabolism
- Plant responses to water stress
- Photosynthetic Processes and Mechanisms
- Plant Physiology and Cultivation Studies
- Postharvest Quality and Shelf Life Management
- Plant-Microbe Interactions and Immunity
- Geological and Geochemical Analysis
- Seismology and Earthquake Studies
- Plant biochemistry and biosynthesis
- Peroxisome Proliferator-Activated Receptors
- Plant Gene Expression Analysis
- Legume Nitrogen Fixing Symbiosis
- Earthquake Detection and Analysis
- Soybean genetics and cultivation
- Microbial Metabolites in Food Biotechnology
- Polysaccharides and Plant Cell Walls
- High-pressure geophysics and materials
- RNA and protein synthesis mechanisms
- Mitochondrial Function and Pathology
- Seismic Waves and Analysis
Nagoya University
2016-2025
Kawasaki Medical School
2023-2025
Nara Medical University
2018-2024
Saitama Medical University
2022
Kurashiki Central Hospital
2022
Kyoto University
1997-2017
Hokkaido University
1999-2016
Mie University
2003-2009
Kōchi University
2006
University of Tsukuba
2006
Summary In intact plants, the shoot apex grows predominantly and inhibits outgrowth of axillary buds. After decapitation apex, buds begins. This phenomenon is called an apical dominance. Although involvement auxin, which represses buds, cytokinin (CK), promotes has been proposed, little known about underlying molecular mechanisms. present study, we demonstrated that auxin negatively regulates local CK biosynthesis in nodal stem by controlling expression level pea ( Pisum sativum L. ) gene...
Significance Grain weight is an important crop yield component; however, its underlying regulatory mechanisms are largely unknown. Here, we identify a grain-weight quantitative trait locus (QTL) in rice encoding new-type GNAT-like protein that harbors intrinsic histone acetyltransferase activity (OsglHAT1). Elevated OsglHAT1 expression enhances grain and by enlarging spikelet hulls via increasing cell number accelerating filling, increases global acetylation levels of H4. Our findings reveal...
Reactive oxygen species (ROS) produced by the NADPH oxidase, respiratory burst oxidase homolog (RBOH), trigger signal transduction in diverse biological processes plants. However, functions of RBOH homologs rice (Oryza sativa) and other gramineous plants are poorly understood. Ethylene induces formation lysigenous aerenchyma, which consists internal gas spaces created programmed cell death cortical cells, roots under oxygen-deficient conditions. Here, we report that, rice, one isoform...
Exposing plants to hypoxic conditions greatly improves their anoxic stress tolerance by enhancing the activities of glycolysis and fermentation in roots. Ethylene may also be involved these adaptive responses because its synthesis is increased roots under conditions. Here it reported that pre-treatment wheat seedlings with an ethylene precursor, 1-aminocyclopropanecarboxylic acid (ACC), enhanced accumulation seedlings, oxygen-deficient through increasing expression genes encoding ethanol...
This report identifies S -RNases of sweet cherry ( Prunus avium L.) and presents information about cDNA sequences encoding the -RNases, which leads to development a molecular typing system for -alleles in this fruit tree species. Stylar proteins were surveyed by two dimensional polyaclylamide gel electrophoresis (2D-PAGE) identify -proteins associated with gametophytic self-incompatibility. Glycoprotein spots linked found group had Mr pI similar those other rosaceous -RNases. These...
A novel isoenzyme of ascorbate peroxidase with molecular mass 31 kDa was found to be localized on membranes microbodies. Intact microbodies had no latent activity, an indication that the active site exposed cytosol and would scavenge hydrogen peroxide leaked from
Abstract The DELLA protein SLENDER RICE1 (SLR1) is a repressor of gibberellin (GA) signaling in rice (Oryza sativa), and most the GA-associated responses are induced upon SLR1 degradation. It assumed that interaction between GIBBERELLIN INSENSITIVE DWARF1 (GID1) N-terminal DELLA/TVHYNP motif triggers F-box GID2-mediated We identified semidominant dwarf mutant, Slr1-d4, which contains mutation region encoding C-terminal GRAS domain (SLR1G576V). GA-dependent degradation SLR1G576V was reduced...
Chloroplasts import thousands of nucleus-encoded preproteins synthesized in the cytosol through TOC and TIC translocons on outer inner envelope membranes, respectively. Preprotein translocation across membrane requires ATP; however, motor has remained unclear. Here, we report that a 2-MD heteromeric AAA-ATPase complex associates with functions as motor, directly interacting various translocating preproteins. This consists protein encoded by previously enigmatic chloroplast gene ycf2 five...
1-aminocyclopropane-1-carboxylic acid (ACC) synthase (ACS) is the rate-limiting enzyme of ethylene biosynthesis pathway. ACS regulated both transcriptionally and post-translationally. We previously reported that LeACS2, a wound-inducible in tomato (Solanum lycopersicum), phosphorylated vivo, suggested phosphorylation regulates protein stability rather than enzymatic activity. In this report, we demonstrate phosphorylation/dephosphorylation LeACS2 its turnover upstream...
Carnivorous plants exploit animals as a nutritional source and have inspired long-standing questions about the origin evolution of carnivory-related traits. To investigate molecular bases carnivory, we sequenced genome heterophyllous pitcher plant Cephalotus follicularis, in which succeeded regulating developmental switch between carnivorous non-carnivorous leaves. Transcriptome comparison two leaf types gene repertoire analysis identified genetic changes associated with prey attraction,...
• Potato (Solanum tuberosum) calcium-dependent protein kinase (StCDPK5) has been shown to phosphorylate the N-terminal region of plasma membrane RBOH (respiratory burst oxidase homolog) proteins, and participate in StRBOHB-mediated reactive oxygen species (ROS) burst. The constitutively active form, StCDPK5VK, provides a useful tool for gain-of-function analysis defense responses. StCDPK5- StCDPK5VK-green fluorescent fusion proteins were predominantly targeted membrane, conditional...
Significance This study investigates a previously unidentified cysteine-rich peptide (CRP). CRPs have diverse roles in plant growth and development, such as control of stomata density guidance pollen-tube elongation. Despite numerous studies on Arabidopsis thaliana , there are still many peptides with unknown function. We identify rice CRP named Regulator Awn Elongation 2 (RAE2) show that it is cleaved specifically the spikelet to promote awn demonstrate RAE2 was target selection during...
Plants tailor immune responses to defend against pathogens with different lifestyles. In this process, antagonism between the hormones salicylic acid (SA) and jasmonic (JA) optimizes transcriptional signatures specifically attacker encountered. Antagonism is controlled by transcription cofactor NPR1. The indispensable role of NPR1 in activating SA-responsive genes well understood, but how it functions as a repressor JA-responsive remains unclear. Here, we demonstrate that SA-induced...
Stylar proteins of 13 almond (Prunus dulcis) cultivars with known S-genotypes were surveyed by IEF and 2D-PAGE combined immunoblot N-terminal amino acid sequence analyses to identify S-RNases associated gametophytic self-incompatibility (SI) in this plant species. RNase activities corresponding Sa Sb, two the four S-alleles tested, identified activity staining. The Sa-RNase band reacted anti-S4serum prepared from Japanese pear (Pyrus serotina); no reaction antiserum was observed sbRNase...
1-Aminocyclopropane-1-carboxylic acid synthase is a key enzyme in the ethylene biosynthesis pathway. Recent studies raise possibility that 1-aminocyclopropane-1-carboxylic (ACS) regulated not only transcriptionally but also post-translationally. To elucidate post-translational ACS regulation, we analyzed modification of LE-ACS2 protein, wound-inducible isozyme family, tomato fruit (Lycopersicon esculentum L.) using an anti-LE-ACS2 antibody. We detected phosphorylated at 55-kDa...
The minimal sequence requirement for a peroxisome-targeting signal was investigated using an in vitro import system. Carboxyl-terminal sequences Ser-Lys-Leu (SKL) and Leu-Gln-Ser-Lys-Leu (LQSKL) of acyl-CoA oxidase (AOX) directed to peroxisomes the fused proteins with import-incompetent forms AOX catalase that had been truncated, implying SKL tripeptide functions as targeting signal. Elimination entire or deletion any 1 2 amino acids abolished activity AOX. Substitution alanine serine did...
Abstract The CRISPR/Cas9 system has been applied to efficient genome editing in many eukaryotic cells. However, the bases that can be edited by this have limited those within protospacer adjacent motif (PAM) and guide RNA-targeting sequences. In study, we developed a genome-wide base technology, “CRISPR Nickase system” utilizes single Cas9 nickase. This was free from limitation of editable observed system, able precisely edit up 53 bp nicking site. addition, showed no off-target editing,...
In roots of gramineous plants, lysigenous aerenchyma is created by the death and lysis cortical cells. Rice (Oryza sativa) constitutively forms under aerobic conditions, its formation further induced oxygen-deficient conditions. However, maize (Zea mays) develops only Ethylene involved in formation. Here, we investigated how ethylene-dependent differently regulated between rice maize. For this purpose, rice, used reduced culm number1 (rcn1) mutant, which ethylene biosynthesis suppressed....
We identified a novel Drosophila protein of ≈ 400kDa, hemolectin (d-Hml), secreted from haemocyte-derived Kc167 cells. Its 11.7kbp cDNA contains an open reading frame 3843 amino acid residues, with conserved domains in von Willebrand factor (VWF), coagulation V/VIII and complement factors. The d-hml gene is located on the third chromosome (position 70C1-5) consists 26 exons. major part d-Hml well-known motifs organization:...
Abstract Ethylene receptors are multispanning membrane proteins that negatively regulate ethylene responses via the formation of a signaling complex with downstream elements. To better understand their biochemical functions, we investigated topology and subcellular localization CmERS1, melon (Cucumis melo) receptor has three putative transmembrane domains at N terminus. Analyses using fractionation green fluorescent protein imaging approaches indicate CmERS1 is predominantly associated...