A5059877323- Genetic diversity and population structure
- Genomics and Phylogenetic Studies
- Chromosomal and Genetic Variations
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- MicroRNA in disease regulation
- Neurobiology and Insect Physiology Research
- Insect Resistance and Genetics
- Amphibian and Reptile Biology
- Animal Behavior and Reproduction
- RNA and protein synthesis mechanisms
- Insect and Arachnid Ecology and Behavior
- Evolution and Genetic Dynamics
- Molecular Biology Techniques and Applications
- Microbial Community Ecology and Physiology
- Invertebrate Immune Response Mechanisms
- Gene expression and cancer classification
- Genetic Mapping and Diversity in Plants and Animals
- Biochemical Analysis and Sensing Techniques
- Plant Diversity and Evolution
- Cancer-related molecular mechanisms research
- Protist diversity and phylogeny
- Silkworms and Sericulture Research
- Plant Molecular Biology Research
- Plant and Biological Electrophysiology Studies
- Marine and coastal plant biology
Tokyo Metropolitan University
2004-2024
National Institute of Genetics
2013-2018
The Graduate University for Advanced Studies, SOKENDAI
2013-2018
National Institute for Basic Biology
2012-2017
Nippon Soken (Japan)
2014-2016
Prefectural University of Hiroshima
2014
Hiroshima University
2014
Kindai University
2009-2013
Pennsylvania State University
2007-2011
Kansai University
2009
The unique anatomical features of turtles have raised unanswered questions about the origin their body plan. We generated and analyzed draft genomes soft-shell turtle (Pelodiscus sinensis) green sea (Chelonia mydas); our results indicated close relationship to bird-crocodilian lineage, from which they split ∼267.9-248.3 million years ago (Upper Permian Triassic). also found extensive expansion olfactory receptor genes in these turtles. Embryonic gene expression analysis identified an...
MicroRNAs (miRNAs) are among the most important regulatory elements of gene expression in animals and plants. However, their origin evolutionary dynamics have not been studied systematically. In this paper, we identified putative miRNA genes 11 plant species using bioinformatic technique examined changes. Our homology search indicated that no is currently shared between green algae land The number has increased substantially lineage, but after divergence eudicots monocots, changed a...
The number of sensory receptor genes varies extensively among different mammalian species. This variation is believed to be caused partly by physiological requirements animals and genomic drift due random duplication deletion genes. If the contribution substantial, each species should contain a significant amount copy (CNV). We therefore investigated CNVs in 270 healthy humans using published CNV data. results indicated that olfactory (OR), taste type 2, vomeronasal 1 show high level...
Natural selection operating in protein-coding genes is often studied by examining the ratio (omega) of rates nonsynonymous to synonymous nucleotide substitution. The branch-site method (BSM) based on a likelihood test one such tests detect positive for predetermined branch phylogenetic tree. However, because number substitutions involved very small, we conducted computer simulation examine reliability BSM comparison with small-sample (SSM) Fisher's exact test. results indicate that generates...
Carnivorous plants exploit animals as a nutritional source and have inspired long-standing questions about the origin evolution of carnivory-related traits. To investigate molecular bases carnivory, we sequenced genome heterophyllous pitcher plant Cephalotus follicularis, in which succeeded regulating developmental switch between carnivorous non-carnivorous leaves. Transcriptome comparison two leaf types gene repertoire analysis identified genetic changes associated with prey attraction,...
Ten pale-pink- and pale-yellow-pigmented, Gram-negative, non-motile, rod-shaped, chemoheterotrophic bacteria designated strains YM20-087T, YM21-151, MN1-741T, YM27-120T, YM26-010T, YM24-184, YM20-122, A4T-83T, A5J-41-2T A5J-40 were isolated from various marine environments subjected to a polyphasic taxonomic investigation. Phylogenetic analyses based on 16S rRNA gene sequences indicated that these isolates belonged the phylum 'Verrucomicrobia' (subdivision 1) represented three independent...
Olfactory receptor (OR) genes are of vital importance for animals to find food, identify mates, and avoid dangers. In mammals, the number OR is large varies extensively among different orders, whereas, in insects, extent interspecific variation appears be small, although only a few species have been studied. To understand evolutionary changes genes, we identified all from 12 Drosophila species, which time roughly equivalent that eutherian mammals. The results showed examined similar numbers...
MicroRNAs (miRs) regulate gene expression at the posttranscriptional level. To obtain some insights into origins and evolutionary patterns of miR genes, we have identified genes in genomes 12 Drosophila species by bioinformatics approaches examined their changes. The results showed that extant ancestral had more than 100 frequent gains losses occurred during evolution. Although many appear to originated from random hairpin structures intronic or intergenic regions, duplication has also...
To clarify the establishment process of coral-algal symbiotic relationships, coral transcriptome changes during increasing algal symbiont densities were examined in juvenile corals following inoculation with algae Symbiodinium goreaui (clade C) and S. trenchii D), comparison their transcriptomes aposymbiotic by RNA-sequencing. Since clades C D showed very different rates density increase, comparisons made early onsets both symbionts, revealing that host behaved differently for each....
Sex chromosome dosage compensation (DC) is widely accepted in various organisms. This concept mostly supported by comparisons of gene expression between chromosomes and sexes. However, genes on the X autosomes are not homologous, average level these may be same even under DC, which complicates chromosomes. Many with sex-biased also make sexes difficult. To overcome issues, we investigated DC comparing neo-X-linked Drosophila pseudoobscura those their autosomal orthologs other species. The...
It is well known that renal cell carcinoma (RCC) represents one of the most immune-responsive cancers. Although lack defined antigens in RCC has hindered more specific vaccine development, research regarding vaccination therapy been special interest for treatment than 30 years. To evaluate safety vascular endothelial growth factor receptor 1 (VEGFR1) peptide and its clinical outcomes, data from 18 metastatic (mRCC) patients treated with VEGFR1 were collected. Toxicity assessments performed....
Over the course of evolution, acquisition novel structures has ultimately led to wide variation in morphology among extant multicellular organisms. Thus, origins genetic systems for new morphological are a subject great interest evolutionary biology. The larval skeleton is structure acquired some echinoderm lineages via activation adult skeletogenic machinery. Previously, VEGF signaling was suggested have played an important role skeleton. In present study, we compared expression patterns...
Amphioxus or lancelets have been regarded as a key animal in understanding the origin of vertebrates. However, evolutionary history within this lineage remains unexplored. As amphioxus has likely separated from other chordates for very long time and displays marked left-right asymmetry, its is potentially helpful better chordate vertebrate origins. We studied phylogenetic relationships extant based on mitochondrial genomes incorporating new Asymmetron Epigonichthys populations, previously...
Endosymbiosis is an important evolutionary event for organisms, and there widespread interest in understanding the evolution of endosymbiosis establishment. Hydra one most suitable organisms studying endosymbiosis. Within genus Hydra, H. viridissima vulgaris show with green algae. Previous studies suggested that took place much more recently than viridissima, noting establishment interaction between its algae not as stable viridissima. To investigate on-going process endosymbiosis, we first...
Sex ratio is distorted when selfish elements linked to the sex chromosome cause meiotic drive. Since first report on drive in Drosophila obscura 1928, this phenomenon has been reported diverse taxa. However, molecular and evolutionary mechanisms of have remained largely elusive, even D. . We therefore reinvestigated original example by comparing strains with without distortion (SR ST strains, respectively). Crossing experiments indicated presence a driver X predicted existence suppressor....
How newly generated microRNA (miRNA) genes are integrated into gene regulatory networks during evolution is fundamental in understanding the molecular and evolutionary bases of robustness plasticity regulation. A recent model proposed that after birth a miRNA, miRNA generally network by decreasing number target evolution. However, this remains to be carefully examined considering vivo conditions. In study, we therefore compared among miRNAs with different ages, combining experiments...
Dosage compensation (DC) on the X Chromosome counteracts deleterious effects of gene loss Y Chromosome. However, DC is not efficient if also degenerates. This indeed occurs in Drosophila miranda, which both neo-Y and neo-X are under accelerated pseudogenization. To examine generality this pattern, we investigated evolution two additional neo-sex chromosomes that emerged independently D. albomicans americana reanalyzed chromosome miranda Comparative genomic transcriptomic analyses revealed...
MicroRNAs (miRs) are noncoding RNAs that regulate gene expression at the post-transcriptional level. In animals, target sites of a miR generally located in 3' untranslated regions (UTRs) messenger RNAs. However, how change during evolution is largely unknown. MiR-iab-4 and miR-iab-4as known to two Hox genes, Abd-A Ubx, Drosophila melanogaster. We have therefore studied evolutionary changes these genes their Drosophila, other insect species, Daphnia. Our homology search identified single copy...
Abstract Eusocial insects have polyphenic caste systems in which each exhibits characteristic morphology and behaviour. In insects, arose independently different lineages, such as Isoptera Hymenoptera. Although partial molecular mechanisms for the development of eusociality termites been clarified by functional analysis genes hormones, contribution microRNAs (miRNAs) to differentiation is unknown. To understand role miRNAs termite polyphenism, we performed small RNA sequencing a subterranean...