A5016320454- Plant-Microbe Interactions and Immunity
- Nuclear Physics and Applications
- Plant Stress Responses and Tolerance
- Legume Nitrogen Fixing Symbiosis
- Plant responses to water stress
- Radiation Detection and Scintillator Technologies
- Electron and X-Ray Spectroscopy Techniques
- Photocathodes and Microchannel Plates
- Nuclear reactor physics and engineering
- Plant Virus Research Studies
- Enzyme Catalysis and Immobilization
- Synthesis and Biological Activity
- Analytical Chemistry and Chromatography
- Plant Pathogenic Bacteria Studies
- Atomic and Subatomic Physics Research
- Particle Accelerators and Free-Electron Lasers
- Photosynthetic Processes and Mechanisms
- Microbial Natural Products and Biosynthesis
- Plant Gene Expression Analysis
- Molecular spectroscopy and chirality
- Nematode management and characterization studies
- Boron Compounds in Chemistry
- Plant Pathogens and Resistance
- Plant tissue culture and regeneration
- Proteoglycans and glycosaminoglycans research
Nagoya University
2010-2024
Ibaraki University
2016
High Energy Accelerator Research Organization
2001-2015
Kyushu University
1993-2011
Tohoku University
1999
Keio University
1995
Pathogen attack sequentially confers pattern-triggered immunity (PTI) and effector-triggered (ETI) after sensing of pathogen patterns effectors by plant immune receptors, respectively. Reactive oxygen species (ROS) play pivotal roles in PTI ETI as signaling molecules. Nicotiana benthamiana RBOHB, an NADPH oxidase, is responsible for both the transient ROS burst robust burst. Here, we show that RBOHB transactivation mediated MAPK contributes to R3a/AVR3a-triggered (AVR3a-ETI) markedly induced...
Reactive oxygen species (ROS) produced by the NADPH oxidase, respiratory burst oxidase homolog (RBOH), trigger signal transduction in diverse biological processes plants. However, functions of RBOH homologs rice (Oryza sativa) and other gramineous plants are poorly understood. Ethylene induces formation lysigenous aerenchyma, which consists internal gas spaces created programmed cell death cortical cells, roots under oxygen-deficient conditions. Here, we report that, rice, one isoform...
Abstract Mitogen-activated protein kinase (MAPK) cascades have pivotal roles in plant innate immunity. However, downstream signaling of defense-related MAPKs is not well understood. Here, we provide evidence that the Nicotiana benthamiana WRKY8 transcription factor a physiological substrate SIPK, NTF4, and WIPK. Clustered Pro-directed Ser residues (SP cluster), which are conserved group I WRKY proteins, N-terminal region were phosphorylated by these vitro. Antiphosphopeptide antibodies...
The brown planthopper (BPH) is the most serious insect pest of rice in Asia. indica cultivar ADR52 carries two BPH resistance genes, BPH26 (BROWN PLANTHOPPER RESISTANCE 26) and BPH25. Map-based cloning revealed that encodes a coiled-coil-nucleotide-binding-site–leucine-rich repeat (CC–NBS–LRR) protein. mediated sucking inhibition phloem sieve element. was identical to BPH2 on basis DNA sequence analysis feeding ability BPH2-virulent biotype BPH. widely incorporated elite cultivars...
• Potato (Solanum tuberosum) calcium-dependent protein kinase (StCDPK5) has been shown to phosphorylate the N-terminal region of plasma membrane RBOH (respiratory burst oxidase homolog) proteins, and participate in StRBOHB-mediated reactive oxygen species (ROS) burst. The constitutively active form, StCDPK5VK, provides a useful tool for gain-of-function analysis defense responses. StCDPK5- StCDPK5VK-green fluorescent fusion proteins were predominantly targeted membrane, conditional...
CALCIUM-DEPENDENT PROTEIN KINASE (CDPK) stimulates reactive oxygen species (ROS)-dependent signaling by activating RESPIRATORY BURST OXIDASE HOMOLOG (RBOH). The lysigenous aerenchyma is a gas space created cortical cell death that facilitates diffusion from the shoot to root tips. Previously, we showed RBOHH indispensable for induction of formation in rice (Oryza sativa) roots under low-oxygen conditions. Here, CDPK5 and CDPK13 localize plasma membrane where functions. Mutation analysis...
Reactive oxygen species (ROS) are rapidly generated during plant immune responses by respiratory burst oxidase homolog (RBOH), which is a plasma membrane-localized NADPH oxidase. Although regulatory mechanisms of RBOH activity have been well documented, the ROS-mediated downstream signaling unclear. We here demonstrated that ROS sensor proteins play central role in via oxidative post-translational modification cysteine residues, sulfenylation. To detect protein sulfenylation, we used...
MEK2-SIPK/WIPK cascade, a Nicotiana benthamiana mitogen-activated protein kinase (MAPK) is an essential signaling pathway for plant immunity and involved in hypersensitive response (HR) accompanied by cell death. WRKY transcription factors as substrates of SIPK WIPK have been isolated implicated HR Here, we show virus-induced gene silencing genes compromised constitutively active MEK2-triggered death N. leaves. In general, enhances susceptibility to necrotrophic pathogens such Botrytis...
Summary Potato antimicrobial sesquiterpenoid phytoalexins lubimin and rishitin have been implicated in resistance to the late blight pathogen, Phytophthora infestans early Alternaria solani . We generated transgenic potato plants which sesquiterpene cyclase, a key enzyme for production of rishitin, is compromised by RNAi investigate role defence. The tubers were deficient exhibited reduced post‐invasive an avirulent isolate P. , resulting successful infection first attacked cells without...
M. Tanaka, Yoshioka and K. Sakai, J. Chem. Soc., Commun., 1992, 1454 DOI: 10.1039/C39920001454
LjABCG1, a full-size ABCG subfamily of ATP-binding cassette proteins model legume, Lotus japonicus, was reported as gene highly expressed during the early stages nodulation, but have not been characterized in detail. In this study we showed that induction LjABCG1 expression remarkable by methyl jasmonate treatment, and reporter experiments indicated strongly nodule parenchyma cell layers adjacent to root vascular tissue toward nodule. suggested be localized at plasma membrane based on...
Recent research has illustrated that signaling networks, after the plant host perceives diverse pathogen-derived signals, facilitate defense responses through mitogen-activated protein kinase (MAPK) cascades, calcium-dependent (CDPK), or receptor-like cytoplasmic (RLCK). The pathogen-induced reactive oxygen species (ROS) burst is mainly caused by activation of an NADPH oxidase, designated as respiratory oxidase homolog (RBOH). Emerging evidence emphasizes NbRBOHB could be activated CDPK-...
Cyanation reactions of carbonyl compounds with methyl cyanoformate or acetyl cyanide catalyzed by 5 mol % 1,5,7-triazabicyclo[4,4,0]dec-5-ene (TBD) were examined. Using cyanoformate, the corresponding cyanohydrin carbonates readily obtained in high yield for aromatic and aliphatic aldehydes ketones. Similar results when was used as source. The polymer-supported catalyst, PS-TBD, also acted a good catalyst this reaction. PS-TBD easily recovered reused minimal activity loss.