A5062442010- Plant tissue culture and regeneration
- Plant biochemistry and biosynthesis
- Legume Nitrogen Fixing Symbiosis
- Plant Gene Expression Analysis
- Bioactive Compounds and Antitumor Agents
- Natural product bioactivities and synthesis
- Plant-Microbe Interactions and Immunity
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Phytochemicals and Antioxidant Activities
- Plant Molecular Biology Research
- Berberine and alkaloids research
- Soybean genetics and cultivation
- Plant Stress Responses and Tolerance
- Phytochemical compounds biological activities
- Bioactive natural compounds
- Synthesis of Organic Compounds
- Agronomic Practices and Intercropping Systems
- Plant Parasitism and Resistance
- Drug Transport and Resistance Mechanisms
- Nematode management and characterization studies
- Light effects on plants
- Plant Reproductive Biology
- Synthesis of Indole Derivatives
- Biochemical and biochemical processes
Kyoto University
2016-2025
United Nations University Institute for Sustainability and Peace
2009-2021
Research Institute for Sustainable Humanosphere
2006-2019
TCL (China)
2012
GTx (United States)
2012
Plant Gene Expression Center
2009
Nihon University
2008
Nara Institute of Science and Technology
2008
Institute of Agrobiological Sciences
2008
Toyo University
2008
Abstract Members of the ABC (for ATP binding cassette) superfamily integral membrane transporters function in cellular detoxification, cell-to-cell signaling, and channel regulation. More recently, members multidrug resistance P-glycoprotein (MDR/PGP) subfamily have been shown to transport phytohormone auxin both monocots dicots. Here, we report that Arabidopsis thaliana MDR/PGP PGP4 functions basipetal redirection from root tip. Reporter gene studies showed was strongly expressed cap...
Highly diverse communities of bacteria inhabiting soybean rhizospheres play pivotal roles in plant growth and crop production; however, little is known about the changes that occur these during growth. We used both culture-dependent physiological profiling culture independent DNA-based approaches to characterize bacterial rhizosphere field. The properties were analyzed by a community-level substrate utilization assay with BioLog Eco plates, composition was assessed gene pyrosequencing....
Plant Gene Register titles for PGR 99–174 to 99–187 appear below. The sequences have beendeposited in GenBank and the articles listed online through World Wide Web.To cite an electronic article as a bibliographic reference, follow stylegiven below:Park S, Thornburg RW (1998) Characterization of UMP kinase cDNAs from rice (accession nos.AF187062 AF187063) (PGR 99–174). Physiol
Alkaloids comprise one of the largest groups plant secondary metabolites. Berberine, a benzylisoquinoline alkaloid, is preferentially accumulated in rhizome Coptis japonica , ranunculaceous plant, whereas gene expression for berberine biosynthetic enzymes has been observed specifically root tissues, which suggests that synthesized transported to rhizome, where there high accumulation. We recently isolated cDNA encoding multidrug-resistance protein (MDR)-type ATP-binding cassette (ABC)...
Alkaloids play a key role in plant defense mechanisms against pathogens and herbivores, but the plants themselves need to cope with their toxicity as well. The major alkaloid of Nicotiana species, nicotine, is translocated via xylem transport from root tissues where it biosynthesized accumulation sites, vacuoles leaves. To unravel molecular behind this membrane transport, we characterized one transporter, tobacco (Nicotiana tabacum) jasmonate-inducible transporter 1 (Nt-JAT1), whose...
Abstract Nicotine is a major alkaloid accumulating in the vacuole of tobacco (Nicotiana tabacum), but transporters involved vacuolar sequestration are not known. We here report that genes (NtMATE1 and NtMATE2) encoding multidrug toxic compound extrusion (MATE) family coordinately regulated with structural for nicotine biosynthesis root, respect to spatial expression patterns, regulation by NIC regulatory loci, induction methyl jasmonate. Subcellular fractionation, immunogold electron...
Abstract Legume plants have an ability to fix atmospheric nitrogen into nutrients via symbiosis with soil microbes. As the initial event of symbiosis, legume secrete flavonoids rhizosphere attract rhizobia. Secretion is indispensable for establishment symbiotic fixation, but almost nothing known about membrane transport mechanism flavonoid secretion from root cells. In this study, we performed biochemical analyses characterize using soybean (Glycine max) in which genistein a signal...
Selected cultured Coptis japonica cells produce a large amount of the benzylisoquinoline alkaloid berberine. Previous studies have suggested that berberine productivity is controlled at transcript level biosynthetic genes. We identified regulator transcription in biosynthesis using functional genomics with transient RNA interference (RNAi) and overexpression candidate gene. The 24 primary clones were selected from 1,014 expressed sequence tags (ESTs) obtained C. cell line producing high...
The phytohormone auxin is critical for plant growth and many developmental processes. Members of the P-glycoprotein (PGP/ABCB) subfamily ATP-binding cassette (ABC) transporters have been shown to function in polar movement by transporting over plasma membrane both monocots dicots. Here, we characterize a new Arabidopsis member ABCB subfamily, ABCB21/PGP21, close homolog ABCB4, which conflicting transport directionalities reported. ABCB21 strongly expressed abaxial side cotyledons junctions...
Summary Nitrogen‐fixing symbiosis between legumes and rhizobia is initiated by the recognition of rhizobial Nod factors (NFs) host plants. NFs are diversely modified derivatives chitin oligosaccharide, a fungal elicitor that induces defense responses in Recent evidence has shown both elicitors recognized structurally related LysM receptor kinases. Transcriptome analyses Lotus japonicus roots indicated not only activate genes but also transiently defense‐related through NF receptors....
Multidrug and toxic compound extrusion (MATE) transporters are a family of cation antiporters occurring in most organisms from prokaryotes to eukaryotes. This constitutes one the largest transporter families plants, with, for example, more than 50 MATE genes Arabidopsis genome. Moreover, involved wide variety physiological functions throughout plant development, transporting broad range substrates such as organic acids, hormones secondary metabolites. review categorizes according their roles...
Furanocoumarins constitute a sub-family of coumarin compounds with important defense properties against pathogens and insects, as well allelopathic functions in plants. are divided into two sub-groups according to the alignment furan ring lactone structure: linear psoralen angular angelicin derivatives. Determination furanocoumarin type is based on prenylation position common precursor all furanocoumarins, umbelliferone, at C6 or C8, which gives rise derivatives, respectively. Here, we...
Summary Suberin is a complex polymer composed of aliphatic and phenolic compounds. It constituent apoplastic plant interfaces. In many species, including rice ( Oryza sativa ), the hypodermis in outer part roots forms suberized cell wall (the Casparian strip and/or suberin lamellae), which inhibits flow water ions protects against pathogens. To date, there no genetic evidence that an transport barrier hypodermis. We discovered reduced culm number1 rcn1 ) mutant could not develop longer than...
Abstract CRISPR/Cas9 is a programmable nuclease composed of the Cas9 protein and guide RNA (gRNA) molecule. To create mutant potato, powerful genome-editing system was required because potato has tetraploid genome. The translational enhancer dMac3, consisting portion OsMac3 mRNA 5′-untranslated region, greatly enhanced production encoded in downstream ORF. enrich amount Cas9, we applied dMac3 to expression with multiple gRNA genes. systems targeting granule-bound starch synthase I ( GBSSI )...
Plant roots nurture a wide variety of microbes via exudation metabolites, shaping the rhizosphere's microbial community. Despite importance plant specialized metabolites in assemblage and function communities rhizosphere, little is known how far effects these extend through soil. We employed fluid model to simulate spatiotemporal distribution daidzein, an isoflavone secreted from soybean roots, validated using soybeans grown rhizobox. then analysed daidzein affects bacterial soils...
S-adenosyl-L-methionine:coclaurine N-methyltransferase (CNMT) converts coclaurine to N-methylcoclaurine in isoquinoline alkaloid biosynthesis. The N-terminal amino acid sequence of Coptis CNMT was used amplify the corresponding cDNA fragment and later isolate full-length using 5'- 3'-rapid amplification ends (RACE). nucleotide predicted showed that encoded 358 acids, which contained a putative S-adenosyl-L-methionine binding domain relatively high homology tomato...
Two cDNAs encoding geranyl diphosphate:4-hy- droxybenzoate 3-geranyltransferase were isolated fromLithospermum erythrorhizon by nested PCR using the conserved amino acid sequences among polyprenyl- transferases for ubiquinone biosynthesis. They functionally expressed in yeastCOQ2 disruptant and showed a strict substrate specificity diphosphate as prenyl donor, contrast to biosynthetic enzymes, suggesting that they are involved biosynthesis of shikonin, naphthoquinone secondary metabolite....
Plants produce a large number of secondary metabolites, such as alkaloids, terpenoids, and phenolic compounds. Secondary metabolites have various functions including protection against pathogens UV light in plants, been used natural medicines for humans utilizing their diverse biological activities. Many these compounds are accumulated particular compartment vacuoles, some even translocated from source cells to sink organs via long distance transport. Both primary transporters involved...
Abstract Prenylated flavonoids are natural compounds that often represent the active components in various medicinal plants and exhibit beneficial effects on human health. hybrid products composed of a flavonoid core mainly attached to either 5-carbon (dimethylallyl) or 10-carbon (geranyl) prenyl groups derived from isoprenoid (terpenoid) metabolism, crucial for their biological activity. Prenylation reactions vivo coupling processes two major metabolic pathways, shikimate-acetate which...