A5078958948- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- CRISPR and Genetic Engineering
- Plant tissue culture and regeneration
- Chromosomal and Genetic Variations
- Genomics and Phylogenetic Studies
- Biofuel production and bioconversion
- Plant Gene Expression Analysis
- RNA and protein synthesis mechanisms
- Plant responses to water stress
- Ocean Waves and Remote Sensing
- Plant Stress Responses and Tolerance
- Tropical and Extratropical Cyclones Research
- Plant Reproductive Biology
- Enzyme Production and Characterization
- Coastal and Marine Dynamics
- Plant Virus Research Studies
- Bacterial Genetics and Biotechnology
- Genetic Mapping and Diversity in Plants and Animals
- Rice Cultivation and Yield Improvement
- GABA and Rice Research
- Biopolymer Synthesis and Applications
- Microbial Community Ecology and Physiology
- Light effects on plants
Tokyo University of Science
2016-2025
Yokohama National University
2024
Bio-Medical Science (South Korea)
2016
Kansai University
2007-2015
Katsushika Medical Center
2014
Tokyo University of Pharmacy and Life Sciences
2009
Kyushu University
2008
Kobe University
2007
California Maritime Academy
2007
Mie Prefectural Education Center
2007
Abstract Rice (Oryza sativa) endosperm accumulates a massive amount of storage starch and proteins during seed development. However, little is known about the regulatory system involved in production substances. The rice flo2 mutation resulted reduced grain size quality. Map-based cloning identified FLOURY ENDOSPERM2 (FLO2), member novel gene family conserved plants, as responsible for mutation. FLO2 harbors tetratricopeptide repeat motif, considered to mediate protein–protein interactions....
Summary Different colors, such as purple, brown, red and white, occur in the pericarp of rice. Here, two genes affecting proanthocyanidin synthesis red‐ brown‐colored rice were elucidated. Genetic segregation analysis suggested that Rd A loci are identical, both encode dihydroflavonol‐4‐reductase (DFR). The introduction DFR gene into an Rcrd mutant resulted red‐colored rice, which was brown original mutant, demonstrating locus encodes protein. Accumulation proanthocyanidins observed...
Summary To study the GH3 gene family of Arabidopsis , we investigated a flanking sequence database activation‐tagged lines. We found dwarf mutant, named y d o k ari 1‐D ( ydk1‐D ), that had T‐DNA insertion proximal to gene. is dominant and has short hypocotyl not only in light but also darkness. Moreover, primary root, reduced lateral root number, apical dominance. A gene, YDK1 was upregulated transgenic plants showed phenotype. expression induced by exogenously applied auxin regulated...
Laser capture microdissection (LCM) is a powerful system which allows the isolation of selectively targeted cells from tissue section for analysis gene-expression profiles individual cells. The technique has been successfully used specific mammalian cells, mainly cancer However, LCM never reported to be applied gene expression plant We modified and it target isolate phloem rice leaf whose morphology apparently different surrounding Total RNA was extracted microdissected (approximately 150)...
Abstract Phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2] functions as a site-specific signal on membranes to promote cytoskeletal reorganization and membrane trafficking. Localization of PtdIns(4,5)P2 apices growing root hairs pollen tubes suggests that it plays an important role in tip growth. However, its regulation mode action remain unclear. We found Arabidopsis thaliana PIP5K3 (for Phosphate 5-Kinase 3) encodes phosphatidylinositol 4-phosphate 5-kinase, key enzyme producing...
Abstract F-box proteins regulate diverse cellular processes, including cell cycle transition, transcriptional regulation and signal transduction, by playing roles in Skp1p-cullin-F-box protein (SCF) complexes or non-SCF complexes. are encoded a large gene family. Our database search revealed that at least 568 genes present the Arabidopsisthaliana (Arabidopsis) genome. Domain analysis using SMART Pfam-A databases 67 of contained Kelch repeats 29 leucine-rich (LRRs). Interestingly only two...
In flowering plants, the tapetum, innermost layer of anther, provides both nutrient and lipid components to developing microspores, pollen grains, coat. Though programmed cell death tapetum is one most critical sensitive steps for fertility affected by various environmental stresses, its regulatory mechanisms remain mostly unknown. Here we show that autophagy required metabolic regulation supply in anthers autophagic degradation within cells essential postmeiotic anther development rice....
Histone acetylation is an essential process in the epigenetic regulation of diverse biological processes, including environmental stress responses plants. Previously, our research group identified a histone deacetylase (HDAC) inhibitor (HDI) that confers salt tolerance Arabidopsis (Arabidopsis thaliana). In this study, we demonstrate class I HDAC (HDA19) and II HDACs (HDA5/14/15/18) control to through different pathways. The screening 12 selective HDIs indicated seven newly reported enhance...
Abstract CRISPR/Cas9 is a programmable nuclease composed of the Cas9 protein and guide RNA (gRNA) molecule. To create mutant potato, powerful genome-editing system was required because potato has tetraploid genome. The translational enhancer dMac3, consisting portion OsMac3 mRNA 5′-untranslated region, greatly enhanced production encoded in downstream ORF. enrich amount Cas9, we applied dMac3 to expression with multiple gRNA genes. systems targeting granule-bound starch synthase I ( GBSSI )...
An antisense gene strategy was applied to suppress the 14–16 kDa allergen expression in maturing rice seeds. Gene constructs producing RNAs of 16 under control some seed‐specific promoters were introduced into by electroporation. Immunoblot and RNA blot analyses seeds from transgenic plants using allergen‐specific monoclonal antibody a sequence‐specific probe demonstrated that proteins their transcripts several lines present much lower amounts than those parental wild‐type rice. The high...
Suc, an end product of photosynthesis, is metabolized by Suc synthase in sink organs as initial step the biosynthesis storage products. activity known to be regulated reversible phosphorylation, but details this process are unclear at present. Rice SPK, a calcium-dependent protein kinase, expressed uniquely endosperm immature seed, and its involvement biosynthetic pathways products was suggested. Antisense SPK transformants lacked ability accumulate such starch, produced watery seed with...
Brassinosteroids (BRs) are plant steroidal hormones that regulate growth and development. An Arabidopsis dwarf mutant, shrink1-D (shk1-D), was isolated the phenotype shown to be caused by activation of CYP72C1 gene. is a member cytochrome P450 monooxygenase gene family similar BAS1/CYP734A1 regulates BR inactivation. shk1-D has short hypocotyls in both light dark, petioles siliques. The seeds also shortened along longitudinal axis indicating controls cell elongation. expression CPD, TCH4...
Rosmarinic acid (RA), one of the main phenolic compounds in sweet basil (Ocimum basilicum L.), has antiviral, antimicrobial, and anti-inflammatory pharmacological properties. As recent studies have shown that UV-B blue light irradiation stimulate phenylpropanoid biosynthesis, we determined whether conditions affect RA content antioxidant activity. Both activity were increased by continuous white irradiation. Ultra performance liquid chromatography analysis a methanolic extract revealed...
We characterized a rice monoculm mutant moc2, which showed significantly reduced tiller numbers, pale-green leaves, growth rate, and consequent dwarf phenotype. The feature was attributed to deficiency in the efficient outgrowth of buds, although moc2 produced buds. Inconsistent change observed expression genes involved bud outgrowth, suggesting that has defective function necessary for outgrowth. gene responsible mapped locus encoding cytosolic fructose-1,6-bisphosphatase 1 (FBP1), Tos17...
Four forms of branching enzyme, termed RBE1, RBE2 (a mixture RBE2A and RBE2B), RBE3, RBE4, were apparently separated by DEAE-cellulose column chromatography soluble extract from immature rice seeds, each these four was further purified gel-filtration. RBE2A, RBE2B the predominant enzyme. The molecular size, amino-terminal amino acid sequence, immunoreactivity with anti-maize enzyme-I (BE-I) antibody identical among three forms, except that mass almost 3 kDa higher than those RBE1 RBE2B....
In order to elucidate the function of cryptochromes (cry) in rice, we have characterized all rice CRY genes, including OsCRY1a, OsCRY1b and OsCRY2. Our studies revealed that OsCRY1 genes were mainly expressed green plant tissue, while OsCRY2 gene expression was high coleoptile, flower callus. Light treatment affected neither any OsCRY nor stability their transcripts. However, it found Oscry2 protein negatively regulated by blue light. Moreover, level also decreased upon red light treatment,...
Two different types of genes for rice GA-stimulated transcript (GAST) homologue genes, Oryza sativa transcript-related gene 1 (OsGASR1) and 2 (OsGASR2), were found. Both OsGASR proteins contain a cysteine-rich domain highly conserved among GAST family in their C-terminal regions. Gibberellin A3 (GA3) stimulated expression both OsGASRs the wild-type Nipponbare GA3 synthesis-deficient mutant. Expression apparently increased when cell proliferation entered logarithmic phase, rapidly reduced was...