A5056424937- Photosynthetic Processes and Mechanisms
- Genomics and Phylogenetic Studies
- RNA and protein synthesis mechanisms
- Plant Molecular Biology Research
- Mitochondrial Function and Pathology
- Protist diversity and phylogeny
- Algal biology and biofuel production
- Microbial Community Ecology and Physiology
- Light effects on plants
- CRISPR and Genetic Engineering
- RNA modifications and cancer
- Plant Genetic and Mutation Studies
- Plant nutrient uptake and metabolism
- Plant Stress Responses and Tolerance
- RNA Research and Splicing
- Plant tissue culture and regeneration
- RNA regulation and disease
- Marine and coastal plant biology
- Chromosomal and Genetic Variations
- Plant Diversity and Evolution
- Plant Gene Expression Analysis
- Plant Disease Resistance and Genetics
- Transgenic Plants and Applications
- Insect Resistance and Genetics
- Plant biochemistry and biosynthesis
Nagoya University
2012-2022
Ajinomoto (United States)
2014
National Center for Gene Research
2001-2013
Tohoku University
2011
RIKEN Center for Emergent Matter Science
2007
The University of Tokyo
2007
National Institute for Basic Biology
2004
Tokyo Metropolitan University
2004
Aichi Gakuin University
1997
Sugiyama Jogakuen University
1997
Vascular plants appeared ~410 million years ago, then diverged into several lineages of which only two survive: the euphyllophytes (ferns and seed plants) lycophytes. We report here genome sequence lycophyte Selaginella moellendorffii (Selaginella), first nonseed vascular plant reported. By comparing gene content in evolutionarily diverse taxa, we found that transition from a gametophyte- to sporophyte-dominated life cycle required far fewer new genes than flowering plant, whereas secondary...
Pentatricopeptide repeat (PPR) proteins form a huge family in plants (450 members Arabidopsis and 477 rice) defined by tandem repetitions of characteristic sequence motifs. Some these have been shown to play role posttranscriptional processes within organelles, they are thought be sequence-specific RNA-binding proteins. The origins this obscure as lacking from almost all prokaryotes, the spectacular expansion land is equally enigmatic. In study, we investigate growth undertaking genome-wide...
The complete nucleotide sequence of the chloroplast genome (150,613 bp) from unicellular green alga Chlorella vulgaris C-27 has been determined. contains no large inverted repeat and one copy rRNA gene cluster consisting 16S, 23S, 5S genes. It 31 tRNA genes, which Leu (GAG) not found in land plant DNAs analyzed so far. Sixty-nine protein genes eight ORFs conserved with those chloroplasts have also found. most striking is existence two adjacent homologous to bacterial involved cell division,...
Abstract The plant-specific DYW subclass of pentatricopeptide repeat proteins has been postulated to be involved in RNA editing organelle transcripts. We discovered that the CHLORORESPIRATORY REDUCTION22 (CRR22) and CRR28 are required for multiple plastid transcripts but their motifs dispensable activity vivo. Replacement CRR22 by CRR2, which shown capable endonucleolytic cleavage, blocks both proteins. In return, neither nor can functionally replace CRR2. propose different family members...
KaiA, KaiB, and KaiC clock proteins from cyanobacteria ATP are sufficient to reconstitute the phosphorylation rhythm in vitro , whereas almost all gene promoters under control of circadian clock. The mechanism by which cycle drives global transcription rhythms is unknown. Here, we report that RpaA, a potential DNA-binding protein acts as cognate response regulator KaiC-interacting kinase SasA, mediates between rhythms. Circadian was severely attenuated sasA ( Synechococcus adaptive sensor A...
The complete chloroplast DNA sequence (122 890 bp) of the moss Physcomitrella patens has been determined. genome contains 83 protein, 31 tRNA and four rRNA genes, a pseudogene. Four protein genes (rpoA, cysA, cysT ccsA) found in liverwort Marchantia polymorpha hornwort Anthoceros formosae are absent from P.patens. overall structure P.patens (cpDNA) differs substantially that hornwort. Compared with its close relatives, 71 kb region petD to rpoB is inverted. To investigate whether this large...
In higher plants, RNA editing is a post-transcriptional process that converts C to U in organelle mRNAs. We have previously shown an Arabidopsis thaliana crr4 mutant defective with respect for creating the translational initial codon of plastid ndhD gene (the ndhD-1 site). CRR4 contains 11 pentatricopeptide repeat motifs but does not contain any domains are likely be involved activity. The green fluorescent protein fused putative transit peptide targeted plastid. recombinant expressed...
In the unicellular cyanobacterium Synechococcus elongatus PCC 7942, essentially all promoter activities are under control of circadian clock continuous light (LL) conditions. Here, we used high-density oligonucleotide arrays to explore comprehensive profiles genome-wide gene expression in wild-type, kaiABC-null, and kaiC-overexpressor strains LL dark (DD) wild-type strains, >30% transcripts oscillated significantly a fashion, peaking at subjective dawn dusk. Such was severely attenuated...
In BT-type cytoplasmic male sterile rice (Oryza sativa L.) with Chinsurah Boro II cytoplasm, sterility (CMS) is caused by an accumulation of the cytotoxic peptide ORF79. The ORF79 protein expressed from a dicistronic gene atp6-orf79, which exists in addition to normal atp6 mitochondrial genome. CMS restored PPR (pentatricopeptide-repeat) gene, Rf1, via RNA processing. However, it has not yet been elucidated how reduced action Rf1 protein. Here, we report that level processed orf79...
Opinions on the basal relationship of land plants vary considerably and no phylogenetic tree with significant statistical support has been obtained. Here, we report analyses using 51 genes from entire chloroplast genome sequences 20 representative green plant species. The analyses, translated amino acid sequences, indicated that extant bryophytes (mosses, liverworts, hornworts) form a monophyletic group high confidence are likely sisters to vascular plants, although for was not strong....
The nucleotide sequence of a tRNA Lys (UUU) gene on tobacco ( Nicotiana tabacum ) chloroplast DNA has been determined. This is located 215 base pairs upstream from the for 32,000-dalton thylakoid membrane protein same strand and 2526-base-pair intron in anticodon loop. boundary does not follow G-U/A-G rule but similar to those split genes Gly (UCC) ribosomal proteins L2 S12. contains one major open reading frame 509 codons. codon usage resembles observed so far analyzed. primary transcript...
The tomato gene family for the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase [3-phospho-D-glycerate carboxy-lyase (dimerizing); EC 4.1.1.39] has five genes, designated Rbcs-1, -2, -3A, -3B, and -3C. We have measured steady-state mRNA levels each genes in various organs using gene-specific oligonucleotides. All are highly expressed leaves, transcripts two Rbcs-3B Rbcs-3C, account approximately equal to 60% total leaf transcripts. relative transcript stem, nature fruits,...
In most land plants RNA editing frequently occurs in many organelle transcripts, but little is known about the molecular mechanisms of process. this study, we have characterized Physcomitrella patens PpPPR_71 gene that required for ccmFc transcript. This transcript harbors two sites, ccmF-1 and ccmF-2, are separated by 18 nucleotides. Complementary DNA sequence analysis suggested at site occurred before ccmF-2 editing. downstream was specifically impaired disruption encodes a polypeptide...
The nucleotide sequence of a tobacco (Nicotiana tabacum) chloroplast gene cluster that encodes eight proteins homologous to Escherichia coli ribosomal L23, L2, S19, L22, S3, L16, L14, and S8 has been determined. RNA gel blot hybridization revealed all coding regions are expressed in the chloroplasts. arrangement genes resembles found E. S10 spc operons. Among genes, L2 L16 contain 666- 1020-base-pair introns, respectively. These intron boundary sequences consistent with conserved group III...
Many plant pentatricopeptide repeat (PPR) proteins are known to contain a highly conserved C‐terminal DYW domain whose function is unknown. Recently, the has been proposed play role in RNA editing organelles. To address this possibility, we prepared recombinant and tested their cytidine deaminase activity. However, could not detect any activity assays used. Instead, found that domains possessed endoribonuclease cleaved before adenosine residues molecule. Some DYW‐containing PPR may catalyze...
Pentatricopeptide repeat (PPR) proteins are encoded by the nuclear genome as a large gene family in land plants. PPR play essential roles organelle-related functions, mostly RNA-processing steps plastids and mitochondria. In moss Physcomitrella patens, there is also family, but likely to be divergent from those of higher To investigate function plastid proteins, we have generated characterized protein disruptant P. patens. The PPR531-11-disrupted mosses displayed abnormal phenotypic...
The pentatricopeptide repeat (PPR) protein family is involved in various steps of RNA metabolism plastids and mitochondria. To investigate the function a DYW sub-class PPR moss Physcomitrella patens, we constructed characterized knockout mutants PpPPR_43 gene, which encodes mitochondrial localized with C-terminal domain. disruptants showed poor growth protonemata. whether transcripts were affected by disruption PpPPR_43, sequenced cDNA to detect editing events performed RT-PCR analyses...