Abstract The CRISPR/Cas9 system has been applied to efficient genome editing in many eukaryotic cells. However, the bases that can be edited by this have limited those within protospacer adjacent motif (PAM) and guide RNA-targeting sequences. In study, we developed a genome-wide base technology, “CRISPR Nickase system” utilizes single Cas9 nickase. This was free from limitation of editable observed system, able precisely edit up 53 bp nicking site. addition, showed no off-target editing,...

The control of seedborne rice seedling diseases in the seed beds is important to avoid epidemics nurseries and paddies, which may result severe yield loss. Recently, irradiation with plasma containing electrons, creating positive or negative ions neutral species, has been shown have an antimicrobial effect, probably via generation reactive oxygen species. This study examines whether two diseases, bakanae disease caused by fungal pathogen Fusarium fujikuroi , bacterial blight Burkholderia...

Abstract Two-dimensional materials, such as layered compounds and nanosheets, have attracted interest for their characteristic structures properties. If materials containing functional organic molecules are synthesized, designed nanosheets can be obtained by exfoliation. Here we show the design synthesis of an amorphous material a conjugated-polymer network, its exfoliation into applications. Copolymerization benzoquinone pyrrole generates random stacks conjugated polymer layers through...

Conducive polymers have a wide range of applications originating from their π-conjugated systems. The redox reactions conductive with doping and dedoping anions been applied to cathodes for charge storage. In contrast, the cations not fully studied in anodes Here, we found that nanostructures polymers, such as polypyrrole (PPy) polythiophene (PTp) derivatives, reversible cations. PPy PTp acted specific capacities 157 44.4 mA h g–1, respectively, at current density 20 g–1. introduction...

Recent advances in morphology design and control of organic polymer materials from nanoscopic to macroscopic scales, larger than the molecular scale, are summarized as crystal-controlled polymerization a new perspective.

Imine-based covalent organic frameworks (COFs) are crystalline porous materials with prospective uses in various devices. However, general bulk synthetic methods usually produce COFs as powders that insoluble most of the common solvents, arising challenges for subsequent molding and fixing these on substrates. Here, we report a novel methodology utilizes an electrogenerated acid (EGA), which is produced at electrode surface by electrochemical oxidation suitable precursor, acting effective...

Marine red alga Laurencia composita was collected from seven sampling locations in Japan, and all samples were identified based on morphological studies as well rbcL sequencing analysis. This is the first report of isolation (‐)‐8‐bromo‐9‐hydroxy‐(E)‐γ‐bisabolene (1) a natural product, bisabolene‐type β‐chamigrene‐type sesquiterpenes found L. Japan. In previous Japanese reports composita, highly oxidized α‐chamigrene‐type isolated their major secondary metabolites. Therefore, these...

<title>Abstract</title> The research and implementation of portable low-cost analytical devices that possess high reproducibility ease operation is still a challenging task, growing field importance, within the research. Herein, we report concept, design optimization microfluidic device based on electrochemiluminescence (ECL) detection can be operated without electricity for purposes. functions exploiting concept streaming potential-driven bipolar electrochemistry, where potential...

Herein, we report a divergent synthesis of cationic azatriphenylene derivatives using orthogonal control thermal and electro-oxidative pyridination, which transforms the single precursor into controlled products with perfect selectivity. Simultaneously, two switchable reactions afford corresponding pyridinium salts different optical properties such as aggregation-induced emission (AIE) aggregation-caused quenching (ACQ) effects.

Photoredox‐catalyzed postfunctionalization of poly(meth)acrylate‐based copolymers bearing phthalimide moieties can produce novel polymer architectures that are inaccessible using polymerization approaches; however, this method is restricted to radical reactions. To expand the scope reactions applicable photoredox‐catalyzed postfunctionalization, study focused on radical–polar crossover process, in which a carbocation equivalent generated react with nucleophiles. The...

Photoredox‐catalyzed postfunctionalization of poly(meth)acrylate‐based copolymers bearing phthalimide moieties can produce novel polymer architectures that are inaccessible using polymerization approaches; however, this method is restricted to radical reactions. To expand the scope reactions applicable photoredox‐catalyzed postfunctionalization, study focused on radical–polar crossover process, in which a carbocation equivalent generated react with nucleophiles. The...

Bipolar electrode (BPE) systems have been attracting increasing attention owing to their cost-effectiveness and wireless design. Recently, we reported the concept of streaming potential-driven BPEs for oxidative electropolymerization aromatic monomers without need an electric power supply. However, our previous system, which used cotton as a filler material, had limitations in inducing suitable potential differences charge amounts. In this study, various electrolytes filling materials flow...

'No.80' (Capsicum chinense) from the Caribbean is a valuable genetic source aspect of its non-pungent and highly aromatic traits. In present study, non-pungency, volatile components, phylogenetic origin were analyzed with another C. chinense cultivar, 'No.2' Brazil, which also but less aromatic. Expressions deduced amino acid sequences acyltransferase (Pun1) normal compared pungent 'Habanero'. Insertions 7-bp 8-bp resulting in frameshift mutations found coding regions putative...

Abstract High‐performance organic energy storage has attracted much interest as a future battery. Organic anode been developed an alternate of graphite in the past decade. However, design strategies are not fully studied for further development. The present work shows experiment‐oriented materials informatics (MI) efficient exploration strategy and new compounds active material high‐performance anode. A few important factors to achieve high specific capacity extracted from training dataset...

The hierarchical porous structures of vinyl polymers, such as polystyrene (PSt) and poly(methyl methacrylate) (PMMA), were derived from morphologies a biomineral through replication. resultant PSt showed enhanced adsorption properties organic molecules hydrophobic adsorbents in an aqueous medium.

Atg15 is a lipase essential for disintegration of autophagic body membranes. In this study, we found that the N-terminal domain alone can travel to vacuole via multivesicular pathway, and identified residues required degradation bodies in C-terminal domain.

The organization states of functional molecules have a significant impact on the properties materials. A variety approaches been studied to obtain well-organized molecular assemblies. present work shows new non-organized state isolated and dispersed in amorphous flexible covalent organic networks. Redox-active quinone are embedded network polymers. Consecutive reactions between benzoquinone (BQ) linker generate random structures through polymerization at different rates multiple directions....