A5079890722- Plant tissue culture and regeneration
- Plant Molecular Biology Research
- CRISPR and Genetic Engineering
- Transgenic Plants and Applications
- Plant Reproductive Biology
- Postharvest Quality and Shelf Life Management
- Plant Virus Research Studies
- Plant Physiology and Cultivation Studies
- Plant Genetic and Mutation Studies
- Chromosomal and Genetic Variations
- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- GABA and Rice Research
- Plant Gene Expression Analysis
- Advances in Cucurbitaceae Research
- Light effects on plants
- Legume Nitrogen Fixing Symbiosis
- Genetically Modified Organisms Research
- Plant nutrient uptake and metabolism
- Biochemical Analysis and Sensing Techniques
- Leaf Properties and Growth Measurement
- Plant-Microbe Interactions and Immunity
- Plant biochemistry and biosynthesis
- Agricultural Practices and Plant Genetics
- Cocoa and Sweet Potato Agronomy
University of Tsukuba
2016-2025
Toranomon Hospital
2025
Innovation Research Center
2019-2024
Padjadjaran University
2022-2023
Toho University
2022
National Center for Gene Research
2013
National Institute for Basic Biology
2009
National Institute for Environmental Studies
2009
University of Miyazaki
2009
Nagoya University
2006
We report a highly efficient protocol for the Agrobacterium-mediated genetic transformation of miniature dwarf tomato (Lycopersicon esculentum), Micro-Tom, model cultivar functional genomics. Cotyledon explants inoculated with Agrobacterium tumefaciens (Rhizobium radiobacter) C58C1Rif(R) harboring binary vector pIG121Hm generated mass chimeric non-transgenic and transgenic adventitious buds. Repeated shoot elongation from buds on selection media resulted in production multiple plants that...
Abstract γ-Aminobutyric acid (GABA) is a non-proteinogenic amino that has hypotensive effects. Tomato ( Solanum lycopersicum L.) among the most widely cultivated and consumed vegetables in world contains higher levels of GABA than other major crops. Increasing these can further enhance blood pressure-lowering function tomato fruit. Glutamate decarboxylase (GAD) key enzyme biosynthesis; it C-terminal autoinhibitory domain regulates enzymatic function, deleting this increases GAD activity. The...
Abstract Parthenocarpy in horticultural crop plants is an important trait with agricultural value for various industrial purposes as well direct eating quality. Here, we demonstrate a breeding strategy to generate parthenocarpic tomato using the CRISPR/Cas9 system. We optimized system introduce somatic mutations effectively into SlIAA9 —a key gene controlling parthenocarpy—with mutation rates of up 100% T0 generation. Furthermore, analysis off-target deep sequencing indicated that our...
An efficient and high yielding expression system is required to produce recombinant proteins. Furthermore, the transient can be used identify localization of proteins in plant cells. In this study, we demonstrated that combination a geminiviral replication double terminator dramatically enhanced protein level plants. The GFP was expressed transiently lettuce, Nicotiana benthamiana, tomatoes, eggplants, hot peppers, melons, orchids with agroinfiltration. Compared single terminator, level. A...
The tomato is an excellent model for studies of plants bearing berry-type fruits and experimental the Solanaceae family due to its conserved genetic organization. In this study, a comprehensive mutant population was generated in background Micro-Tom, dwarf, rapid-growth variety. previous studies, including 8,598 6,422 M2 mutagenized lines produced by ethylmethane sulfonate (EMS) mutagenesis γ-ray irradiation, study developed investigated these alteration visible phenotypes. A total 9,183...
Salinity stress enhances sugar accumulation in tomato (Solanum lycopersicum) fruits. To elucidate the mechanisms underlying this phenomenon, transport of carbohydrates into fruits and regulation starch synthesis during fruit development plants cv. 'Micro-Tom' exposed to high levels salinity were examined. Growth with 160 mM NaCl doubled compared control early stages development, soluble sugars increased as matured. Tracer analysis 13C confirmed that elevated carbohydrate was confined did not...
The Solanaceae family includes several economically important vegetable crops. tomato (Solanum lycopersicum) is regarded as a model plant of the family. Recently, number resources have been developed in parallel with ongoing genome sequencing project. In particular, miniature cultivar, Micro-Tom, system genomics, and genomics Micro-Tom-background, such ESTs mutagenized lines, established by an international alliance. To accelerate progress we collection fully-sequenced 13,227 Micro-Tom...
A large amount of γ-aminobutyric acid (GABA) was found to accumulate in tomato (Solanum lycopersicum) fruits before the breaker stage. Shortly thereafter, GABA rapidly catabolized after We screened GABA-rich cultivar 'DG03-9' which did not show rapid catabolism Although hyperaccumulation and is well known, mechanisms are clearly understood. In order clarify these mechanisms, we performed comparative studies 'Micro-Tom' for analysis gene expression levels, protein levels enzymatic activity...
To accelerate functional genomic research in tomato, we developed a Micro-Tom TILLING (Targeting Induced Local Lesions In Genomes) platform. DNA pools were constructed from 3,052 ethyl methanesulfonate (EMS) mutant lines treated with 0.5 or 1.0% EMS. The mutation frequency was calculated by screening 10 genes. 0.5% EMS population had mild of one per 1,710 kb, whereas the 737 suitable for producing an allelic series mutations target overall 1,237 which affected average three alleles kilobase...
Summary Fruit set in angiosperms marks the transition from flowering to fruit production and a commitment seed dispersal. Studies with Solanum lycopersicum (tomato) have shown that pollination subsequent fertilization induce biosynthesis of several hormones, including auxin gibberellins ( GA s), which stimulate set. Circumstantial evidence suggests gaseous hormone ethylene may also influence set, but this has yet be substantiated molecular or mechanistic data. Here, we examined at...
Melatonin exists in a considerable variety of plant species. However, the physiological roles melatonin plants are not well understood. In this study, distribution and accumulation during leaf fruit development were analyzed Micro-Tom, model cultivar tomato (Solanum lycopersicum L.). was extracted using an acetone-methanol method measured by enzyme-linked immunosorbent assay. detected leaves, stems, roots, flowers, fruits, seedlings seeds range 1.5-66.6 ng/g fresh weight, with containing...
Steroidal glycoalkaloids (SGAs) are cholesterol-derived specialized metabolites produced in species of the Solanaceae. Here, we report that a group jasmonate-responsive transcription factors ETHYLENE RESPONSE FACTOR (ERF) family (JREs) close homologs alkaloid regulators Cathranthus roseus and tobacco, regulate production SGAs tomato. In transgenic tomato, overexpression dominant suppression JRE genes caused drastic changes SGA accumulation expression for metabolic enzymes involved multistep...
Xanthophylls, the pigments responsible for yellow to red coloration, are naturally occurring carotenoid compounds in many colored tissues of plants. These esterified within chromoplast; however, little is known about mechanisms underlying their accumulation flower organs. In this study, we characterized two allelic tomato (Solanum lycopersicum L.) mutants, pale petal (pyp) 1-1 and pyp1-2, that have reduced color intensity petals anthers due loss-of-function mutations. Carotenoid analyses...
We report an improved assembly (v3.6.1) of the melon (Cucumis melo L.) genome and a new annotation (v4.0). The optical mapping approach allowed correcting order orientation 21 previous scaffolds permitted to correctly define gap-size extension along 12 pseudomolecules. A comprehensive was also built in update v3.5.1, released more than six years ago. Using integrative pipeline, based on exhaustive RNA-Seq collections ad-hoc transposable element annotation, we identified 29,980 protein-coding...
The function of the plant hormone jasmonic acid (JA) in development tomato (Solanum lycopersicum) flowers was analyzed with a mutant defective JA perception (jasmonate-insensitive1-1, jai1-1). In contrast Arabidopsis (Arabidopsis thaliana) JA-insensitive plants, which are male sterile, jai1-1 is female major defects development. To identify putative JA-dependent regulatory components, we performed transcriptomics on ovules from at three developmental stages wild type and mutants. One...
Abstract We isolated two muskmelon (Cucumis melo) cDNA homologs of the Arabidopsis ethylene receptor genes ETR1 andERS1 and designated them Cm-ETR1(C.meloETR1; accession no. AF054806) andCm-ERS1 (C.meloERS1; AF037368), respectively. Northern analysis revealed that level Cm-ERS1mRNA in pericarp increased parallel with increase fruit size then markedly decreased at end enlargement. In fully enlarged Cm-ERS1 mRNA was low all tissues, whereas Cm-ETR1 very high seeds placenta. During ripening...
Inhibitors of ethylene synthesis or its physiological function enhanced nodulation in Lotus japonicus and Macroptilium atropurpureum. In contrast, the application 1-aminocyclopropane-1-carboxylic acid, a precursor biosynthesis, reduced nodule number these legumes. These results suggest that an ethylene-mediated signaling pathway is involved process even determinate nodulators.
Taste‐modifying proteins are a natural alternative to artificial sweeteners and flavor enhancers have been used in some cultures for centuries. The taste‐modifying protein, miraculin, has the unusual property of being able modify sour taste into sweet taste. Here, we report use plant expression system production miraculin. A synthetic gene encoding miraculin was placed under control constitutive promoters transferred lettuce. Expression this transgenic lettuce resulted accumulation...
As metabolomics can provide a biochemical snapshot of an organism's phenotype it is promising approach for charting the unintended effects genetic modification. A critical obstacle this application inherently limited metabolomic coverage any single analytical platform. We propose using multiple platforms direct acquisition interpretable data set estimable chemical diversity. example, we report our multi-platform that assesses substantial equivalence tomatoes over-expressing taste-modifying...
A major class of disease resistance (R) genes which encode nucleotide binding and leucine rich repeat (NB-LRR) proteins have been used in traditional breeding programs for crop protection. However, it has difficult to functionally transfer NB-LRR-type R taxonomically distinct families. Here we demonstrate that a pair Arabidopsis (Brassicaceae) genes, RPS4 RRS1, properly function two other Brassicaceae, Brassica rapa napus, but also Solanaceae, Nicotiana benthamiana tomato (Solanum...