A5016894257- Metabolomics and Mass Spectrometry Studies
- Plant Gene Expression Analysis
- Plant biochemistry and biosynthesis
- Fermentation and Sensory Analysis
- Postharvest Quality and Shelf Life Management
- Phytochemicals and Antioxidant Activities
- Plant Stress Responses and Tolerance
- Horticultural and Viticultural Research
- Plant nutrient uptake and metabolism
- Spectroscopy and Chemometric Analyses
- Plant Physiology and Cultivation Studies
- Photosynthetic Processes and Mechanisms
- Crop Yield and Soil Fertility
- Isotope Analysis in Ecology
- Mycotoxins in Agriculture and Food
- Plant tissue culture and regeneration
- GABA and Rice Research
- Biochemical and biochemical processes
- Advanced Chemical Sensor Technologies
- Plant responses to water stress
- Plant Ecology and Soil Science
- Fungal Biology and Applications
- Genetic Mapping and Diversity in Plants and Animals
- Cassava research and cyanide
- Plant Molecular Biology Research
Mycologie et Sécurité des Aliments
2023-2025
Biologie du Fruit et Pathologie
2015-2024
Université de Bordeaux
2014-2023
MetaboHUB
2010-2023
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2010-2023
Metabo (Switzerland)
2019
Institut National de la Recherche Agronomique
2008-2015
Laboratoire Physiologie Cellulaire & Végétale
2008
Genetics and Improvement of Fruit and Vegetables
2007
Abstract To assess the influence of environment on fruit metabolism, tomato (Solanum lycopersicum ‘Moneymaker’) plants were grown under contrasting conditions (optimal for commercial, water limited, or shaded production) and locations. Samples harvested at nine stages development, 36 enzyme activities central metabolism measured as well protein, starch, major metabolites, such hexoses, sucrose, organic acids, amino acids. The most remarkable result was high reproducibility throughout...
• Variations in tissue development and spatial composition have a major impact on the nutritional organoleptic qualities of ripe fleshy fruit, including melon (Cucumis melo). To gain deeper insight into mechanisms involved these changes, we identified key metabolites for rational food quality design. The metabolome, volatiles mineral elements were profiled employing an unprecedented range complementary analytical technologies. Fruits followed at number time points during final ripening...
Xanthophylls, the pigments responsible for yellow to red coloration, are naturally occurring carotenoid compounds in many colored tissues of plants. These esterified within chromoplast; however, little is known about mechanisms underlying their accumulation flower organs. In this study, we characterized two allelic tomato (Solanum lycopersicum L.) mutants, pale petal (pyp) 1-1 and pyp1-2, that have reduced color intensity petals anthers due loss-of-function mutations. Carotenoid analyses...
Summary Tomato is a model organism to study the development of fleshy fruit including ripening initiation. Unfortunately, few studies deal with brief phase accelerated associated respiration climacteric because practical problems involved in measuring respiration. Because constraint‐based modelling allows predicting accurate metabolic fluxes, we investigated and energy dissipation pericarp at breaker stage using detailed stoichiometric respiratory pathway, alternative oxidase uncoupling...
A detailed study of the diurnal compositional changes was performed in tomato (Solanum lycopersicum cv. Moneymaker) leaves and fruits. Plants were cultivated a commercial greenhouse under two growth conditions: control shaded. Expanding fruits closest mature harvested during different day/night cycles (cloudy or sunny day). High-throughput robotized biochemical phenotyping major compounds, as well proton nuclear magnetic resonance mass spectrometry metabolomic profiling, used to measure...
Abstract In Northern Europe, sowing maize one‐month earlier than current agricultural practices may lead to moderate chilling damage. However, studies of the metabolic responses low, non‐freezing, temperatures remain scarce. Here, genetically‐diverse hybrids ( Zea mays, dent inbred lines crossed with a flint line) were cultivated in growth chamber at optimal temperature and then three decreasing for 2 days each, as well field. Leaf metabolomic proteomic profiles determined. chamber, 50%...
Current crop yield of the best ideotypes is stagnating and threatened by climate change. In this scenario, understanding wild plant adaptations in extreme ecosystems offers an opportunity to learn about new mechanisms for resilience. Previous studies have shown species specificity metabolites involved adaptation harsh environments. Here, we combined multispecies ecological metabolomics machine learning-based generalized linear model predictions link metabolome environment a set 24 belonging...
Abstract Background Improvements in the techniques for metabolomics analyses and growing interest metabolomic approaches are resulting generation of increasing numbers profiles. Platforms required profile management, as a function experimental design, metabolite identification, to facilitate mining corresponding data. Various databases have been created, including organism-specific knowledgebases analytical technique-specific spectral databases. However, there is currently no platform...
Root-knot nematodes (RKN) are obligatory plant parasitic worms that establish and maintain an intimate relationship with their host plants. During a compatible interaction, RKN induce the redifferentiation of root cells into multinucleate hypertrophied giant essential for nematode growth reproduction. These metabolically active feeding constitute exclusive source nutrients nematode. Detailed analysis glutathione (GSH) homoglutathione (hGSH) metabolism demonstrated importance these compounds...
Nitrate reductase (NR) is the first enzyme of nitrogen reduction pathway in plants, leading to production ammonia. However, nitrogen-fixing symbiosis between legumes and rhizobia, atmospheric (N2) directly reduced ammonia by bacterial nitrogenase, which questions role NR symbiosis. Next that, best-characterized source nitric oxide (NO) NO known be produced during In present study, we surveyed three genes (MtNR1, MtNR2, MtNR3) Medicago truncatula genome addressed their expression, activity,...
is a major plant pathogen that causes devastating diseases of cereals and produces type B trichothecene (TCTB) mycotoxins in infected grains. A comprehensive understanding the molecular biochemical mechanisms underlying regulation TCTB biosynthesis required for improving strategies to control contamination crops ensuring these do not favor production other toxic metabolites by
Cell fate maintenance is an integral part of plant cell differentiation and the production functional cells, tissues, organs. Fleshy fruit development characterized by accumulation water solutes in enlarging cells parenchymatous tissues. In tomato (Solanum lycopersicum), this process associated with endoreduplication mesocarp cells. The mechanisms that preserve developmental program, once initiated, remain unknown. We show here analysis a previously identified ethyl methanesulfonate-induced...
Abstract Background Abiotic stresses in plants include all the environmental conditions that significantly reduce yields, like drought and heat. One of most significant effects they exert at cellular level is accumulation reactive oxygen species, which cause extensive damage. Plants possess two mechanisms to counter these molecules, i.e. detoxifying enzymes non-enzymatic antioxidants, many classes specialized metabolites. Sunflower, fourth global oilseed, considered moderately resistant....
Central metabolism is the engine of plant biomass, supplying fruit growth with building blocks, energy, and biochemical cofactors. Among metabolic cornerstones, nicotinamide adenine dinucleotide (NAD) particularly pivotal for electron transfer through reduction-oxidation (redox) reactions, thus participating in a myriad processes. Besides redox functions, NAD now assumed to act as an integral regulator signaling cascades involved environmental responses. However, regulation during...
Abstract Juice was prepared from cider apples of the cultivar “Kermerrien” under oxidative conditions. After isolation by solid‐phase extraction, phenolic fraction subjected to high‐performance liquid chromatography/electrospray ionisation mass spectrometry. SIM scans were performed at m/z values obtained in model solutions. The oxidation products, resulting coupling between a molecule caffeoylquinic acid and acid, catechin or dimeric flavan‐3‐ol, detected. Copyright © 2004 John Wiley &...
Plant and crop metabolomic analyses may be used to study metabolism across genetic environmental diversity. Complementary analytical strategies are useful for investigating metabolic changes searching biomarkers of response or performance. The experimental material consisted in eight sunflower lines with two line status, four restorers (R, as males) maintainers (B, corresponding females) routinely hybrid varietal production, respectively complement maintain the cytoplasmic male sterility...
Abstract Introduction Accuracy of feature annotation and metabolite identification in biological samples is a key element metabolomics research. However, the process often hampered by lack spectral reference data experimental conditions, as well logistical difficulties management exchange annotations between laboratories. Objectives To design an open-source infrastructure allowing hosting both nuclear magnetic resonance (NMR) mass spectra (MS), with ergonomic Web interface services to...
This chapter contains sections titled: Introduction Preparation and characterization of the caffeoylquinic acid o-quinone solution Incubation in model solutions LC-MS analysis oxidation products commercial apple beverages Conclusions Acknowledgments References