A5071672311- Plant-Microbe Interactions and Immunity
- Plant Stress Responses and Tolerance
- Metabolomics and Mass Spectrometry Studies
- Photosynthetic Processes and Mechanisms
- Plant Parasitism and Resistance
- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Plant Physiology and Cultivation Studies
- Plant biochemistry and biosynthesis
- Postharvest Quality and Shelf Life Management
- Plant tissue culture and regeneration
- Plant Virus Research Studies
- Microbial Community Ecology and Physiology
- Plant Pathogens and Fungal Diseases
- Plant Gene Expression Analysis
- Sirtuins and Resveratrol in Medicine
- Soil Carbon and Nitrogen Dynamics
- Calcium signaling and nucleotide metabolism
- Plant Pathogenic Bacteria Studies
- Insect-Plant Interactions and Control
- Plant responses to elevated CO2
- Mycorrhizal Fungi and Plant Interactions
- Genomics and Phylogenetic Studies
- Horticultural and Viticultural Research
- Genetic Mapping and Diversity in Plants and Animals
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2025
Biologie du Fruit et Pathologie
2018-2025
MetaboHUB
2018-2025
Université de Bordeaux
2018-2025
University of Sheffield
2014-2020
Metabo (Switzerland)
2019
Université Paris-Saclay
2011-2018
Université Paris Cité
2016-2018
Université Paris-Sud
2011-2018
Institut National de la Recherche Agronomique
2018
Abstract The rhizobiome is an important regulator of plant growth and health. Plants shape their communities through production release primary secondary root metabolites. Benzoxazinoids (BXs) are common tryptophan-derived metabolites in grasses that regulate belowground aboveground biotic interactions. In addition to biocidal activity, BXs can plant–biotic interactions as semiochemicals or within-plant defence signals. However, the full extent mechanisms by which root-associated microbiome...
Summary Rhizosphere chemistry is the sum of root exudation chemicals, their breakdown products and microbial soil‐derived chemicals. To date, most studies about are based on sterile cultivation systems, which limits discovery that act as semiochemicals shape rhizosphere communities. Here, we present a method for untargeted metabolic profiling non‐sterile soil. We have developed an experimental growth system enables collection analysis chemicals from different plant species. High‐throughput...
Many metabolic processes that occur in living cells involve oxidation-reduction (redox) chemistry underpinned by compounds, such as glutathione, ascorbate, and/or pyridine nucleotides. Among these redox carriers, nicotinamide adenine dinucleotide (NAD+) represents one of the cornerstones cellular oxidation and is essential for plant growth development. In addition to its role, compelling evidence a role NAD+ signal molecule plants emerging. non-plant systems, biosynthesis can be product two...
Abstract Among crop fruit trees, the apricot ( Prunus armeniaca ) provides an excellent model to study divergence and adaptation processes. Here, we obtain nearly 600 Armeniaca genomes four high-quality assemblies anchored on genetic maps. Chinese European apricots form two differentiated gene pools with high diversity, resulting from independent domestication events distinct wild Central Asian populations, subsequent flow. A relatively low proportion of genome is affected by selection....
Ascorbate (vitamin C) is an essential antioxidant in fresh fruits and vegetables. To gain insight into the regulation of ascorbate metabolism plants, we studied mutant tomato plants (Solanum lycopersicum) that produce ascorbate-enriched fruits. The causal mutation, identified by a mapping-by-sequencing strategy, corresponded to knock-out recessive mutation class photoreceptor named PAS/LOV protein (PLP), which acts as negative regulator biosynthesis. This trait was confirmed CRISPR/Cas9 gene...
Summary Plant development and function are underpinned by redox reactions that depend on co‐factors such as nicotinamide adenine dinucleotide (NAD). NAD has recently been shown to be involved in several signalling pathways associated with stress tolerance or defence responses. However, the mechanisms which influences plant gene regulation, metabolism physiology still remain unclear. Here, we took advantage of Arabidopsis thaliana lines overexpressed nadC from E. coli , encodes biosynthesis...
Pyridine nucleotides, such as NAD, are crucial redox carriers and have emerged important signaling molecules in stress responses. Previously, we demonstrated Arabidopsis (Arabidopsis thaliana) that the inducible NAD-overproducing nadC lines more resistant to an avirulent strain of Pseudomonas syringae pv tomato (Pst-AvrRpm1), which was associated with salicylic acid-dependent defense. Here, further characterized NAD-dependent immune response Arabidopsis. Quinolinate-induced stimulation...
Summary β‐Aminobutyric acid ( BABA ) induces broad‐spectrum disease resistance, but also represses plant growth, which has limited its exploitation in crop protection. perception relies on binding to the aspartyl‐ tRNA synthetase (Asp RS IBI 1, primes enzyme for secondary defense activity. This study aimed identify structural analogues that induce resistance without stunting growth. Using site‐directed mutagenesis, we demonstrate l )‐aspartic acid‐binding domain of 1 is critical perception....
Necrotrophic and biotrophic pathogens are resisted by different plant defenses. While necrotrophic sensitive to jasmonic acid (JA)-dependent resistance, salicylic (SA)- reactive oxygen species (ROS)-dependent resistance. Although many switch from biotrophy necrotrophy during infection, little is known about the signals triggering this transition. This study based on observation that early colonization pattern symptom development ascomycete pathogen Plectosphaerella cucumerina (P. cucumerina)...
Ascorbate is a major antioxidant buffer in plants. Several approaches have been used to increase the ascorbate content of fruits and vegetables. Here, we combined forward genetics with mapping-by-sequencing using an ethyl methanesulfonate (EMS)-mutagenized Micro-Tom population identify putative regulators underlying high-ascorbate phenotype tomato fruits. Among ascorbate-enriched mutants, family highest fruit level (P17C5, up 5-fold wild-type level) had strongly impaired flower development...
Current crop yield of the best ideotypes is stagnating and threatened by climate change. In this scenario, understanding wild plant adaptations in extreme ecosystems offers an opportunity to learn about new mechanisms for resilience. Previous studies have shown species specificity metabolites involved adaptation harsh environments. Here, we combined multispecies ecological metabolomics machine learning-based generalized linear model predictions link metabolome environment a set 24 belonging...
Minimizing losses to pests and diseases is essential for producing sufficient food feed the world's rapidly growing population. The necrotrophic fungus Botrytis cinerea triggers devastating pre‐ post‐harvest yield in tomato ( Solanum lycopersicum ). Current control methods are based on pre‐harvest use of fungicides, which limited by strict legislation. This investigation tested whether induction resistance β‐aminobutyric acid BABA ) at different developmental stages provides an alternative...
Tomato is a major crop suffering substantial yield losses from diseases, as fruit decay at postharvest level can claim up to 50% of the total production worldwide. Due environmental risks fungicides, there an increasing interest in exploiting plant immunity through priming, which adaptive strategy that improves defensive capacity by stimulating induced mechanisms. Broad-spectrum defence priming be triggered compound ß-aminobutyric acid (BABA). In tomato plants, BABA induces resistance...
Abstract In response to various stimuli, plants acquire resistance against pests and/or pathogens. Such acquired or induced allows rapidly adapt their environment. Spraying the bark of mature Norway spruce ( Picea abies ) trees with phytohormone methyl jasmonate (MeJA) enhances tree‐killing beetles and associated phytopathogenic fungi. Analysis chemical defenses beetle colonization success suggests that MeJA treatment both directly induces immune responses primes inducible for a faster...
Plants deposit photosynthetically-fixed carbon in the rhizosphere, thin soil layer directly around root, thereby creating a hospitable environment for microbes. To manage inhabitants of this nutrient-rich environment, plant roots exude and dynamically adjust microbe-attracting -repelling compounds to stimulate specific members microbiome. Previously, we demonstrated that foliar infection Arabidopsis thaliana by biotrophic downy mildew pathogen Hyaloperonospora arabidopsidis (Hpa) leads...
Metabolomics is now a mature phenotyping tool that provides substantial results within various scientific communities. Its application at large-scale, i.e. on large populations and/or samples, has shown its power for research activities from plant science to human epidemiology and medicine, but it still needs key methodological developments routine application. Here, we review the current state of large-scale metabolomics applications, providing recent examples cohort studies in...
Protein synthesis and degradation are essential processes that regulate cell status. Because labeling in bulky organs, such as fruits, is difficult, we developed a modeling approach to study protein turnover at the global scale developing tomato (Solanum lycopersicum) fruit. Quantitative data were collected for transcripts proteins during fruit development. Clustering analysis showed smaller changes abundance compared mRNA abundance. Furthermore, transcript poorly correlated, coefficient of...
Summary The ascorbate–glutathione (ASC–GSH) cycle is at the heart of redox metabolism, linking major buffers with central metabolism through processing reactive oxygen species (ROS) and pyridine nucleotide metabolism. Tomato fruit development underpinned by changes in buffer contents their associated enzyme capacities, but interactions between them remain unclear. Based on quantitative data obtained for core we built an enzyme‐based kinetic model to calculate metabolite concentrations...