A5041540725- Plant Molecular Biology Research
- Plant Reproductive Biology
- DNA Repair Mechanisms
- Microtubule and mitosis dynamics
- Plant nutrient uptake and metabolism
- Plant tissue culture and regeneration
- Photosynthetic Processes and Mechanisms
- Genomics and Phylogenetic Studies
- Chromosomal and Genetic Variations
- Plant Taxonomy and Phylogenetics
- Retinoids in leukemia and cellular processes
- Genomics and Chromatin Dynamics
- Genetics, Bioinformatics, and Biomedical Research
- Molecular Biology Techniques and Applications
- Plant Genetic and Mutation Studies
- Transgenic Plants and Applications
- Soybean genetics and cultivation
- Autophagy in Disease and Therapy
- Wheat and Barley Genetics and Pathology
- Gene expression and cancer classification
- Plant Gene Expression Analysis
- Plant responses to water stress
- Cancer Genomics and Diagnostics
- Cellular transport and secretion
- Cancer-related Molecular Pathways
Universität Hamburg
2016-2024
Institut de Biologie Moléculaire des Plantes
2011-2017
Université de Strasbourg
2011-2017
Centre National de la Recherche Scientifique
2011-2017
Centre de Gestion Scientifique
2010
University of Tübingen
1998-2001
Polycomb repressive complex 2 (PRC2) is a key regulator of epigenetic states catalyzing histone H3 lysine 27 trimethylation (H3K27me3), chromatin mark. PRC2 composition conserved from humans to plants, but the function during early stage plant life unclear beyond fact that it required for development endosperm, nutritive tissue supports embryo growth. Circumventing requirement in endosperm allowed us generate viable homozygous null mutants FERTILIZATION INDEPENDENT ENDOSPERM (FIE), which...
Cytosine methylation is crucial for gene regulation and silencing of transposable elements in mammals plants. While this epigenetic mark extensively reprogrammed the germline early embryos mammals, extent to which DNA reset between generations plants remains largely unknown. Using Arabidopsis as a model, we uncovered distinct dynamics over element sequences during stages plant development. Specifically, their relics show invariably high at CG sites but increasing CHG CHH sites. This non-CG...
Article6 August 2016Open Access Transparent process The plant-specific CDKB1-CYCB1 complex mediates homologous recombination repair in Arabidopsis Annika K Weimer Department of Molecular Mechanisms Phenotypic Plasticity, Institut de Biologie Moléculaire des Plantes du CNRS, IBMP-CNRS UPR2357, Université Strasbourg, Strasbourg Cedex, France Search for more papers by this author Sascha Biedermann Hirofumi Harashima orcid.org/0000-0003-3370-4111 RIKEN Center Sustainable Resource Science,...
Cytokinesis requires membrane fusion during cleavage-furrow ingression in animals and cell plate formation plants. In Arabidopsis, the Sec1 homologue KEULE (KEU) cytokinesis-specific syntaxin KNOLLE (KN) cooperate to promote vesicle division plane. Here, we characterize AtSNAP33, an Arabidopsis of t-SNARE SNAP25, that was identified as a KN interactor yeast two-hybrid screen. AtSNAP33 is ubiquitously expressed membrane-associated protein accumulated at plasma colocalized with forming plate....
Abstract The retinoblastoma protein (Rb), which typically functions as a transcriptional repressor of E2F‐regulated genes, represents major control hub the cell cycle. Here, we show that loss Arabidopsis Rb homolog RETINOBLASTOMA ‐ RELATED 1 ( RBR 1) leads to death, especially upon exposure genotoxic drugs such environmental toxin aluminum. While death can be suppressed by reduced cell‐proliferation rates, rbr1 mutant cells exhibit elevated levels DNA lesions, indicating direct role in...
The DNA of all organisms is constantly damaged by physiological processes and environmental conditions. Upon persistent damage, plant growth cell proliferation are reduced. Based on previous findings that RBR1, the only Arabidopsis homolog mammalian tumor suppressor gene retinoblastoma, plays a key role in damage response plants, we unravel here network RBR1 interactors under stress This led to identification homologs every DREAM component Arabidopsis, including previously not recognized...
Chromosome axis-associated HORMA domain proteins (HORMADs), e.g. ASY1 in Arabidopsis, are crucial for meiotic recombination. ASY1, as other HORMADs, is assembled on the axis at early meiosis and depleted when homologous chromosomes synapse. Puzzlingly, both processes catalyzed by AAA+ ATPase PCH2 together with its cofactor COMET. Here, we show that remodeling complex temporally spatially differently assembled. While COMET appear to directly interact cytoplasm meiosis, recruited transverse...
Seed development in angiosperms is dependent on the interplay among different transcriptional programs operating embryo, endosperm, and maternally-derived seed coat. In angiosperms, embryo endosperm are products of double fertilization during which two pollen sperm cells fuse with egg cell central female gametophyte. Arabidopsis, analyses mutants cell-cycle regulator CYCLIN DEPENDENT KINASE A;1 (CKDA;1) have revealed importance a paternal genome for effective ultimately seed. Here we...
Retinoblastoma (pRb) is a multifunctional regulator, which was likely present in the last common ancestor of all eukaryotes. The Arabidopsis pRb homolog RETINOBLASTOMA RELATED 1 (RBR1), similar to its animal counterparts, controls not only cell proliferation but also implicated developmental decisions, stress responses and maintenance genome integrity. Although most functions pRb-type proteins involve chromatin association, genome-wide understanding RBR1 binding sites still missing. Here, we...
Flowering plants contain a large number of cyclin families, each containing multiple members, most which have not been characterized to date.Here, we analyzed the role B1 subclass mitotic cyclins in cell cycle control during Arabidopsis development.While reveal CYCB1;5 be pseudogene, remaining four members were found expressed dividing cells.Mutant analyses showed complex pattern overlapping, developmentspecific requirements B1-type with CYCB1;2 playing central role.The double mutant cycb1;1...
Abstract Homologous recombination (HR) is a key DNA damage repair pathway that tightly adjusted to the state of cell. A central regulator homologous conserved helicase‐containing Bloom syndrome complex, renowned for its crucial role in maintaining genome integrity. Here, we show Arabidopsis thaliana , complex activity controlled by selective autophagy. We find recently identified KNO1 facilitates K63‐linked ubiquitination RMI1, structural component thereby triggering RMI1 autophagic...
The Aurora B kinase, encoded by the
The chromosomal passenger complex (CPC) is a heterotetrameric regulator of eukaryotic cell division, consisting an Aurora-type kinase and scaffold built INCENP, Borealin, Survivin. While most CPC components are conserved across eukaryotes, orthologs the chromatin reader Survivin have previously only been found in animals fungi, raising question how its essential role carried out other eukaryotes. By characterizing proteins that bind to Arabidopsis Borealin ortholog, we identified BOREALIN...
Abstract Flowering plants contain a large number of cyclin families, each containing multiple members, most which have not been characterized to date. Here, we analyzed the role B1 subclass mitotic cyclins in cell cycle control during Arabidopsis development. While reveal CYCB1;5 be pseudogene, remaining four members were found expressed dividing cells. Mutant analyses showed complex pattern overlapping, development-specific requirements B1-type with CYCB1;2 playing central role. The double...
Abstract The chromosomal passenger complex (CPC) is a heterotetrameric regulator of eukaryotic cell division, consisting an Aurora-type kinase and scaffold built INCENP, Borealin Survivin. While most CPC components are conserved across eukaryotes, orthologs the chromatin reader Survivin have previously only been found in animals fungi, raising question how its essential role carried out other eukaryotes. By characterizing proteins that bind to Arabidopsis ortholog, we identified BOREALIN...
Abstract Chromosome axis-associated HORMA domain proteins (HORMADs), e.g., ASY1 in Arabidopsis, are crucial for meiotic recombination. ASY1, as other HORMADs, is assembled on the axis at early meiosis and depleted when homologous chromosomes synapse. Puzzlingly, both processes catalyzed by AAA+ ATPase PCH2 together with its cofactor COMET. Here, we show that remodeling complex temporally spatially differently assembled. While COMET appear to directly interact cytoplasm meiosis, recruited...
Abstract The DREAM complex is a key transcriptional regulator especially involved in the control of cell cycle and development. Here, we characterise two novel plant- specific components, FLIC FLAC, which identified through tandem affinity purification experiments as interactors conserved core constituents. We demonstrate that plants lacking both FLAC exhibit pleiotropic phenotypes, including stunted growth reduced fertility. Notably, flic flac double mutants show an early-flowering...
SUMMARY A key regulator of cell division in all eukaryotes is the kinase Aurora B, which encoded by 3 (AUR3) gene Arabidopsis. B has at least two central functions during division. On one hand, it essential for correct, i.e. balanced, segregation chromosomes mitosis and meiosis controlling kinetochore function. other acts plane, where necessary to complete cytokinesis. To accomplish these spatially distinct functions, animals guided its sites action Borealin, INCENP, Survivin that build...