A5083412665- Reproductive Biology and Fertility
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Renal and related cancers
- Prenatal Screening and Diagnostics
- Liver Disease Diagnosis and Treatment
- Alzheimer's disease research and treatments
- Microtubule and mitosis dynamics
- Neuroinflammation and Neurodegeneration Mechanisms
- Epigenetics and DNA Methylation
- Amyotrophic Lateral Sclerosis Research
- Telomeres, Telomerase, and Senescence
- Genomics and Chromatin Dynamics
- Chronic Lymphocytic Leukemia Research
- Mitochondrial Function and Pathology
- Polysaccharides Composition and Applications
- PARP inhibition in cancer therapy
- Nuclear Receptors and Signaling
- Adipose Tissue and Metabolism
- Histone Deacetylase Inhibitors Research
- Digestive system and related health
- Genetics, Aging, and Longevity in Model Organisms
- GDF15 and Related Biomarkers
- Nanoplatforms for cancer theranostics
- Plant Genetic and Mutation Studies
Psychogenics (United States)
2022-2024
Czech Academy of Sciences, Institute of Animal Physiology and Genetics
2016-2023
Veterinary Research Institute
2014-2023
Central European Institute of Technology
2016-2023
St. Anne's University Hospital Brno
2019-2022
University Hospital Brno
2019-2022
International Clinical Research Center, St. Anne's University Hospital Brno
2018-2020
Masaryk University
2016
Hepatocellular carcinoma (HCC) is a leading cause of cancer-related death, which develops in the context fibrosis and cirrhosis caused by chronic inflammation, turn due to non-alcoholic fatty liver disease (NAFLD), alcohol consumption and/or hepatitis viral infection. An increased number senescent cells are associated with age-related tissue degeneration during NAFLD-induced HCC, or chemotherapeutic treatment. Senolytic agents target selectively cells. A combination senolytic drugs dasatinib...
Nonalcoholic fatty liver disease (NAFLD) is increasingly prevalent and represents a growing challenge in terms of prevention treatment. A minority affected patients develops inflammation, subsequently fibrosis, cirrhosis hepatocellular carcinoma (HCC). HCC leading cause cancer-related death. An increased number senescent cells correlate with age-related tissue degeneration during NAFLD-induced HCC. Senolytics are promising agents that target selectively cells. Previous studies showed whereas...
Abstract INTRODUCTION Amyloid precursor protein (APP) undergoes striking changes following traumatic brain injury (TBI). Considering its role in the control of gene expression, we investigated whether APP regulates transcription and translation TBI. METHODS We assessed morphology ( n = 4–9 mice/group), transcriptome 3 proteome behavior 17–27 mice/group) wild‐type (WT) knock‐out (KO) mice either untreated or 10‐weeks RESULTS After TBI, WT displayed transcriptional programs consistent with...
Abstract Aim Astrocytes respond to stressors by acquiring a reactive state characterized changes in their morphology and function. Molecules underlying astrogliosis, however, remain largely unknown. Given that several studies observed increase the Amyloid Precursor Protein (APP) astrocytes, we here test whether APP plays role astrogliosis. Methods We investigated instigates astroglios examining vitro vivo function of naive APP‐deficient astrocytes response well‐established stressors. Results...
Staining mice tissues for β-galactosidase activity is a fundamental tool to detect age- or disease-associated cellular senescence. However, reported analyses of positivity senescence-associated other markers senescence in post-mitotic cells healthy murine have been fragmentary inconclusive. Here, we attempted independently deepen this knowledge using multiple within the same wild type entering middle age (9 months age). A histochemistry protocol pH-dependent detection several was used. At pH...
GDF11 is a member of the TGF-β superfamily that was recently implicated as potential "rejuvenating" factor, which can ameliorate metabolic disorders. The main objective presented study to closely characterize role signaling in glucose homeostasis and differentiation white adipose tissue.We performed microscopy imaging, biochemical transcriptomic analyses tissues 9 weeks old ob/ob mice murine human pre-adipocyte cell lines.Our vivo experiments employing treatment showed improved...
Preimplantation mouse embryo development involves temporal-spatial specification and segregation of three blastocyst cell lineages: trophectoderm, primitive endoderm epiblast. Spatial separation the outer-trophectoderm lineage from two other inner-cell-mass (ICM) lineages starts with 8- to 16-cell transition concludes at 32-cell stages. Accordingly, ICM is derived primary secondary contributed cells; debated relative EPI versus PrE potencies. We report generation but not populations highly...
Proper assembly of the spindle apparatus is crucially important for faithful chromosome segregation during anaphase. Thanks to effort over last decades, we have very detailed information about many events leading and segregation, however still do not understand certain aspects, including, example, length control. When tight regulation size lost, errors emerge. Currently, there are several hypotheses trying explain molecular mechanism The number kinetochores, activity rulers, intracellular...
Background & aims: Growth Differentiation Factor 11 (GDF11) is an anti-aging factor, yet its role in liver diseases not established.We evaluated the of GDF11 healthy conditions and transition from non-alcoholic fatty disease (NAFLD) to steatohepatitis (NASH).Results: mRNA levels positively correlated with NAFLD activity score CPT1, SREBP, PPARγ Col1A1 levels, associated portal fibrosis, morbidly obese patients NAFLD/NASH.GDF11treated mice showed mildly exacerbated hepatic collagen...
Aneuploidy is the most frequent single cause leading into termination of early development in human and animal reproduction. Although mouse frequently used as a model organism for studying aneuploidy, we have only incomplete information about frequency numerical chromosomal aberrations throughout development, usually limited to particular stage or assumed from occurrence micronuclei. In our study, systematically scored aneuploidy vivo embryos, zygotes up 16-cell stage, using kinetochore...
Although in vitro maturation (IVM) of oocytes has been used for a relatively long time, during which the culture conditions have improved remarkably, resulting germ cells are still not fully comparable to obtained from ovary many important aspects, namely fertilization rate and subsequent embryonic development. Some differences between IVM vivo (IVV) were already discovered, including variability spindle assembly morphology. In this study we focused on role molecular motor Kif11 (hereafter...
Abstract Objectives Chemotherapeutic drugs induce senescence in cancer cells but, unlike replicative or oncogene‐induced senescence, do so rather inefficiently and depending on DNA damage. A thorough understanding of the biology chemotherapy‐induced senescent requires their isolation from a mixed population adjacent non‐senescent cells. Materials methods We have developed optimized rapid iodixanol (OptiPrep)‐based gradient centrifugation system to identify, isolate characterize doxorubicin...
Experimental studies in flies, mice, and humans suggest a significant role of impaired axonal transport the pathogenesis Alzheimer's disease (AD). The mechanisms underlying these impairments transport, however, remain poorly understood. Here we report that Swedish familial AD mutation causes standstill Amyloid Precursor Protein (APP) axons at expense its reduced anterograde transport. reflects perturbed directionality APP, which spends significantly more time traveling retrograde direction....
Chromosome segregation in mammalian oocytes is prone to errors causing aneuploidy with consequences such as precocious termination of development or severe developmental disorders. Aneuploidy also represents a serious problem procedures utilizing gametes and early embryos vitro. In our study, we focused on congression defects during meiosis I observed whole nondisjoined bivalents II direct consequence, together substantially delayed first polar body extrusion. We show that the are...
A recent work by Nishina et al. provides new evidence that DPP4 inhibitors exert antitumor effects against hepatocellular carcinoma (HCC) orchestrating immune responses.