A5003272601- Spectroscopy Techniques in Biomedical and Chemical Research
- Advanced Fluorescence Microscopy Techniques
- Photoacoustic and Ultrasonic Imaging
- Marine Sponges and Natural Products
- Spectroscopy and Chemometric Analyses
- Optical Imaging and Spectroscopy Techniques
- Calcium Carbonate Crystallization and Inhibition
- Marine Biology and Environmental Chemistry
- thermodynamics and calorimetric analyses
- Optical Coherence Tomography Applications
- Immune Response and Inflammation
- Nerve injury and regeneration
- Animal Nutrition and Physiology
- Metabolomics and Mass Spectrometry Studies
- Diatoms and Algae Research
- MicroRNA in disease regulation
- Chemical synthesis and alkaloids
- Cardiac Valve Diseases and Treatments
- Mycotoxins in Agriculture and Food
- Molecular Biology Techniques and Applications
- Acute Myeloid Leukemia Research
- Fermentation and Sensory Analysis
- Microbial Natural Products and Biosynthesis
- Tissue Engineering and Regenerative Medicine
- Silk-based biomaterials and applications
TU Dresden
2016-2025
University Hospital Carl Gustav Carus
2016-2025
Universidade Estadual de Ponta Grossa
2023
Università Cattolica del Sacro Cuore
2009-2021
Hospital de Clínicas Universidade Federal do Paraná
2020-2021
Universidade Federal do Paraná
2020-2021
National Center for Tumor Diseases
2020
Deutsche Gesellschaft für Anästhesiologie und Intensivmedizin
2015
University of the Sacred Heart
2014
Istituto Pasteur
2004-2013
MicroRNAs (miRNAs) are small noncoding RNAs, 19–24 nucleotides in length, that regulate gene expression and expressed aberrantly most types of cancer. MiRNAs also have been detected the blood cancer patients can serve as circulating biomarkers. It has shown secreted miRNAs within exosomes be transferred from cell to receiving cells by canonical binding their target messenger RNAs. Here we show tumor-secreted miR-21 miR-29a function another mechanism, ligands receptors Toll-like receptor...
Toll-like receptors (TLRs) are known to play a key role in the innate immune system particularly inflammatory response against invading pathogens. Recent reports strongly indicate that they important roles cancer cells. Prostate represents one of most common for which no cure is available once metastatic and androgen refractory. Since TLR3 has been recently suggested as possible therapeutic target some cell lines, we studied expression functionality two human prostate LNCaP PC3. We report...
Tumor microenvironment plays a central role in the development and dissemination of cancer cells. In addition to study each specific cellular component microenvironment, it has become clear that is type amount information cells exchange ultimately affects phenotype. Recently, been discovered intercellular communication occurs through release microvesicles exosomes, whose cargo represents released by one cell recipient cell. A key this represented microRNAs (miRNAs), small non-coding RNAs...
In order to provide an alternative day-old chick culling in the layer hatcheries, a noninvasive method for egg sexing is required at early stage of incubation before onset embryo sensitivity. Fluorescence and Raman spectroscopy blood offers potential precise contactless ovo sex determination domestic chicken (Gallus gallus f. dom.) eggs already during fourth day. However, such kind optical requires window shell, thus invasive leads decreased hatching rates. Here, we show that near infrared...
Sponges are a valuable source of natural compounds and biomaterials for many biotechnological applications. Marine sponges belonging to the order Verongiida known contain both chitin biologically active bromotyrosines. Aplysina archeri (Aplysineidae: Verongiida) is well bromotyrosines with relevant bioactivity against human animal diseases. The aim this study was develop an express method production naturally prefabricated 3D bromotyrosine-containing extracts simultaneously. This new based...
Abstract TLRs play a crucial role in early host defense against invading pathogens. In the seminiferous epithelium, Sertoli cells are somatic nurse that mechanically segregate germ cell autoantigens by means of blood-tubular barrier and create microenvironment protects from both interstitial ascending The objective this study was to examine TLR expression their functional responses specific agonists mouse cells. We measured TLR2, TLR4, TLR5, TLR6 mRNAs confirmed FACS analysis presence...
β-Chitinous scaffolds isolated from the skeleton of marine cephalopod Sepia officinalis were used as a template for in vitro formation ZnO under conditions (70 °C) which are extreme biological materials. Novel β-chitin/ZnO film-like composites prepared first time by hydrothermal synthesis, and thoroughly characterized using numerous analytical methods including Raman spectroscopy, HR-TEM XRD. We demonstrate growth hexagonal nanocrystals on β-chitin substrate. Our chitin/ZnO presented this...
Toll-like receptors (TLRs) recognize microbial/viral-derived components that trigger innate immune response and conflicting data implicate TLR agonists in cancer, either as protumor or antitumor agents. We previously demonstrated TLR3 activation mediated by its agonist poly(I:C) induces signaling, leading to apoptosis of prostate cancer cells LNCaP PC3 with much more efficiency the former than second aggressive line. The transcription factor hypoxia-induciblefactor 1 (HIF-1)regulates several...
Chitinous scaffolds isolated from the skeleton of marine sponge Aplysina cauliformis were used as a template for in vitro formation zirconium dioxide nanophase ammonium zirconium(IV) carbonate (AZC) under extreme conditions (150 °C). These novel zirconia–chitin based composites prepared first time using hydrothermal synthesis, and thoroughly characterized plethora analytical methods. The thermostability chitinous 3D matrix makes it ideal use synthesis monoclinic nanostructured precursors...
Background Coherent anti-Stokes Raman scattering (CARS) microscopy provides fine resolution imaging and displays morphochemical properties of unstained tissue. Here, we evaluated this technique to delineate identify brain tumors. Methods Different human tumors (glioblastoma, metastases melanoma breast cancer) were induced in an orthotopic mouse model. Cryosections investigated by CARS tuned probe C-H molecular vibrations, thereby addressing the lipid content sample. microspectroscopy was...
Multiphoton imaging has evolved as an indispensable tool in cell biology and holds prospects for clinical applications. When addressing endogenous signals such coherent anti-Stokes Raman scattering (CARS) or second harmonic generation, it requires intense laser irradiation that may cause photodamage. We report increasing fluorescence signal upon multiphoton constitutes a marker of The effect was studied on mouse brain vivo ex vivo, human tissue samples, well glioblastoma cells vitro,...
We report a strategy for preserving the structural features of collagen triple helix in turbostratic graphite.
Abstract Background Metabolic activity alternates between high and low states during different stages of an organism’s life cycle. During the transition from growth to quiescence, a major metabolic shift often occurs oxidative phosphorylation glycolysis gluconeogenesis. We use entry Caenorhabditis elegans into dauer larval stage, developmentally arrested stage formed in response harsh environmental conditions, as model study global changes underlying molecular mechanisms associated with...
Toll-like receptors (TLRs) recognize pathogen-associated molecular patterns and elicit antimicrobial immune responses. In the testis, viruses can induce pathological conditions, such as orchitis, may participate in etiology of testicular cancer; however, mechanisms involved remain under investigation. It has been suggested that because they constitutively express interferon (IFN)-inducible antiviral proteins, Sertoli cells defense system. Previously, we demonstrated a key function mouse...
Abstract TLRs boost antimicrobial response mechanisms by epithelial cells and represent the first line of defense at mucosal sites. In view these immunomodulatory properties, TLR stimulation may a novel means to activate anticancer immune responses. present study, ability ligands affect recruitment different cell populations human prostate cancer lines underlying were investigated. We showed that LNCaP DU-145 express functionally active TLR3 TLR5. Treatment with their respective agonists,...
Toll-like receptor 3 (TLR3) is a key effector of the innate immune system against viruses. Activation TLR3 exerts an antitumoral effect through mechanism action still poorly understood. Here we show that activation by polyinosinic:polycytidylic acid induces up-regulation microRNA-29b, -29c, -148b, and -152 in tumor-derived cell lines primary tumors. In turn, these microRNAs induce reexpression epigenetically silenced genes targeting DNA methyltransferases. DU145 TRAMP-C1 prostate MDA-MB-231...
Chitin of poriferan origin is a unique and thermostable biological material. It also represents an example renewable materials source due to the high regeneration ability Aplysina sponges under marine ranching conditions. Chitinous scaffolds isolated from skeleton sponge aerophoba were used as template for in vitro formation Fe2O3 conditions (pH ∼ 1.5, 90 °C) which are extreme materials. Novel chitin–Fe2O3 three dimensional composites, have been prepared first time using hydrothermal...