In order to provide an alternative day-old chick culling in the layer hatcheries, a noninvasive method for egg sexing is required at early stage of incubation before onset embryo sensitivity. Fluorescence and Raman spectroscopy blood offers potential precise contactless ovo sex determination domestic chicken (Gallus gallus f. dom.) eggs already during fourth day. However, such kind optical requires window shell, thus invasive leads decreased hatching rates. Here, we show that near infrared...

The precise identification and preservation of functional brain areas during neurosurgery are crucial for optimizing surgical outcomes minimizing postoperative deficits. Intraoperative imaging plays a vital role in this context, offering insights that guide surgeons protecting critical cortical regions. We aim to evaluate compare the efficacy intraoperative thermal (ITI) optical (IOI) detecting primary somatosensory cortex, providing detailed assessment their potential integration into...

Abstract The in ovo sexing of chicken eggs is a current task and prerequisite to overcome the mass killing male day-old chicks from laying lines. Although various methods have been developed tested recent years, practicable for sex determination are still missing which can be applicated poultry hatcheries before embryo capable nociception pain sensation. Optical spectroscopic enable an early sex. In this study, novel method based on two-wavelength fluorescence excitation described. More than...

In comparison to mammals, zebrafish are able regenerate many organs and tissues, including the central nervous system (CNS). Within CNS-derived neural retina, light lesions result in a loss of photoreceptors subsequent activation Müller glia, retinal stem cells. glia-derived progenitors differentiate eventually restore anatomical tissue architecture within 4 weeks. However, little is known about how impair vision functionally, as well what extent visual function restored during course...

To date, there is still a lack of reliable imaging modalities to improve the quality consultation, diagnostic and medical examinations oral mucosa in dentistry. Even though, optical technologies have become an important element for detection treatment different diseases soft tissue, case screenings evidence benefit comparison conventional histopathology mostly pending. One promising technology diagnostics coherence tomography (OCT). prove potential OCT, even amount freely accessible OCT data...

In vivo determination of 3-D and dynamic geometries alveolar structures with adequate resolution is essential for developing numerical models the lung. A thorax window prepared in anesthetized rabbits by removal muscle tissue between third fourth rib without harming parietal pleura. The transparent pleura allows contact-free imaging intravital microscopy (IVM) optical coherence tomography (3-D OCT). We demonstrate that dislocation lung surface small enough to observe identical regions...

Mechanical ventilation has the potential to increase inflammation in both healthy and injured lungs. Several animal studies have shown that variable recruits lungs reduces inflammation. However, it is unclear which cellular mechanisms are involved those findings. We hypothesized stretch of LPS-stimulated alveolar epithelial cells (AECs) production pro-inflammatory cytokines compared non-variable stretch. AECs were subjected or cyclic (sinusoidal pattern), with without LPS stimulation. The...

This study presents a highly miniaturized, handheld probe developed for rapid assessment of soft tissue using optical coherencetomography (OCT). OCT is non-invasive technology capable visualizing the sub-surface structural changes that occur in disease such as oral lichen planus. However, usage cavity has been limited, requirements high-quality scanning have often resulted probes are heavy, unwieldy and clinically impractical. In this paper, we present novel combines an all-fiber design with...

Abstract The investigation of lung dynamics on alveolar scale is crucial for the understanding and treatment diseases, such as acute injury ventilator induced injury, to promote development protective ventilation strategies. One approach this establishment numerical simulations tissue mechanics where detailed knowledge about three‐dimensional structure changes during cycle required. We suggest four‐dimensional optical coherence tomography (OCT) imaging a promising modality visualizing...

Time-resolved thermography is a novel method to assess thermal variations and heterogeneities in tissue blood. The recent generation of cameras provides sensitivity less than mK. This high conjunction with non-invasive, label-free radiation-free monitoring makes promising tool for intrasurgical diagnostics. In brain surgery, time-resolved can be employed distinguish between normal anomalous tissue. this study, we investigated discussed the potential neurosurgery intraoperative detection...

Abstract Histone H3 serine 28 (H3S28) phosphorylation and de-repression of polycomb repressive complex (PRC)-mediated gene regulation is linked to stress conditions in mitotic post-mitotic cells. To better understand the role H3S28 vivo , we studied a Drosophila strain with ectopic expression constitutively-activated H3S28A, which prevents PRC2 binding at H3S28, thus mimicking phosphorylation. H3S28A mutants showed prolonged life span improved resistance against starvation paraquat-induced...

Malignant transformation of oral lichen planus (OLP) into squamous cell carcinoma is considered as one the most serious complications OLP. For early detection cancer in OLP follow-up, accurate localization center still difficult but often required for confirmatory biopsy with histopathological examination. Optical coherence tomography (OCT) offers potential more reliable sampling cavity it capable non-invasively imaging degenerated layer structure. In this case-series study 15 patients,...

Abstract We resume the research for contactless in ovo sex determination of domestic chicken, which has final aim to provide an alternative day-old cockerels culling layer industry. In Raman and fluorescence spectroscopy blood eggs incubated until day 3.5 enables correct sexing rates over 90% barely affecting hatching rate. Full automatization processes guarantee high speed fulfill industrial demands is required allow transferring technology inside hatcheries next future.

Dermanyssus gallinae, the poultry red mite (PRM), is a hematophagous temporary ectoparasite that causes serious economic losses and animal health impairment on laying hen farms worldwide. Control limited by parasite's hidden lifestyle, restrictions use of chemical acaricides development resistance against certain drug classes. As result, research was conducted to explore alternative control methods. In recent years, atmospheric pressure plasma has been increasingly reported as an for pest...

Multimodal medical image fusion combines information of one or more images in order to improve the diagnostic value. While previous applications mainly focus on merging from computed tomography, magnetic resonance imaging (MRI), ultrasonic and single-photon emission we propose a novel approach for registration preoperative 3D MRI with intraoperative 2D infrared thermography. Image-guided neurosurgeries are based neuronavigation systems, which further allow us track position orientation...

An intraoperative imaging system facilitating the localisation and characterisation of functional areas, pathological tissue, or perfusion disorders, could enormously support medical decisions during neurosurgical interventions and, thus, reduce risk for patients. To provide both structural information brain tissue to surgeon, a novel multimodal approach based on measurement long-wave infrared radiation visual-light is very promising. In this contribution, we discuss various methods...

The structure of domain walls (DW) in ferroelectric media is great interest as this material used for frequency doublers and other applications. We show that the DWs can nicely be visualized by high resolution optical coherence tomography (OCT). While group refractive index lithium niobate allows a much better than 1 µm, large dispersion blur image has to compensated. Therefore, we developed an adaptive compensation algorithm based on maximizing intensity DWs. By measuring DWs, mean period...

Little is known about mechanical processes of alveolar tissue during ventilation. Optical coherence tomography (OCT) as a three-dimensional and high-resolution imaging modality can be used to visualize subpleural alveoli artificial The quality OCT images increased by matching the refractive index inside one via liquid-filling. Thereby, scattering loss decreased higher penetration depth contrast achieved. We show liquid-filling structures verified optical intravital microscopy (IVM)...

In this paper, the correction of motion-induced high frequency artifacts on sequences thermographic images by a real-time capable approach based Cellular Nonlinear Networks (CNN) is evaluated. For comparison, an offline analysis subspaces presented and results will be compared. Both simulated data sets as well recorded during neurosurgery are considered inputs two measures

In brain surgery, respiration motion, outliers, and occlusions create artefacts in Infrared Thermography (IRT) imaging. this paper, we propose a robust method to handle multiple IRT images which consists of two phases: preprocessing image motion estimation. the phase, Region Interest (RoI) segmentation is employed extract cortex only. Thereafter, Phase Correlation compensate for large followed by occlusion masking based on an approach applying Cellular Nonlinear Networks (CNN). Next,...

Aortic valve sclerosis is characterized as the thickening of aortic without obstruction left ventricular outflow. It has a prevalence 30% in people over 65 years old. represents cardiovascular risk marker because it may progress to moderate or severe stenosis. Thus, early recognition and management are cardinal importance. We examined geometry structure from healthy C57Bl6 wild type age-matched hyperlipidemic ApoE-/- mice with using optical coherence tomography (OCT) multiphoton microscopy...