Abstract The investigation of lung dynamics on alveolar scale is crucial for the understanding and treatment diseases, such as acute injury ventilator induced injury, to promote development protective ventilation strategies. One approach this establishment numerical simulations tissue mechanics where detailed knowledge about three‐dimensional structure changes during cycle required. We suggest four‐dimensional optical coherence tomography (OCT) imaging a promising modality visualizing...

The worldwide incidence of cutaneous melanoma (CM) has been continuously increasing over the last decades. Primary and secondary prevention, with attention to risk factors early diagnosis, remain cornerstone for reducing burden melanoma. Detailed information respect clinical pathological data on is scarce in Brazil.

Although several strategies exist for a minimal-invasive treatment of patients with lung failure, the mortality rate acute respiratory distress syndrome still reaches 30% at minimum. This striking number indicates necessity understanding dynamics on an alveolar level. To investigate dynamical behavior microscale, we used three-dimensional geometrical and functional imaging to observe tissue parameters including size length embedded elastic fibers during ventilation. We established combined...

Little is known about mechanical processes of alveolar tissue during ventilation. Optical coherence tomography (OCT) as a three-dimensional and high-resolution imaging modality can be used to visualize subpleural alveoli artificial The quality OCT images increased by matching the refractive index inside one via liquid-filling. Thereby, scattering loss decreased higher penetration depth contrast achieved. We show liquid-filling structures verified optical intravital microscopy (IVM)...

Cell transplantation and stem cell therapy are promising approaches for regenerative medicine of interest to researchers clinicians worldwide. However, currently, no imaging technique that allows three-dimensional in vivo inspection therapeutically administered cells host tissues is available. Therefore, we investigate magnetomotive optical coherence tomography (MM-OCT) labeled with magnetic particles as a potential noninvasive tracking method. We develop mesenchymal future monitoring. Cells...

Resumo Introdução A mortalidade por causas externas está entre as principais de óbito no mundo, com acentuada expressão em faixas etárias jovens. Objetivo Descrever o perfil das mortes município do Sul país e verificar sua tendência nos últimos anos. Método Estudo retrospectivo dos óbitos usando os coeficientes sexo, grupo etário causa da morte análise regressão quasi Poisson. Resultados O coeficiente foi 46,3 100 mil habitantes, predominando acidentes transporte terrestre (40%) agressões...

Cutaneous melanoma accounts for up to 80% of deaths caused by skin cancer. Diagnostic suspicion and access medical care early intervention in suspected cases is vital the patient's prognosis.

Intravital microscopy (IVM) is a well-established imaging technique for real-time monitoring of microscale lung tissue dynamics. Although accepted as gold standard in respiratory research, its characteristic image features are scarcely understood, especially when trying to determine the actual position alveolar walls. To allow correct interpretation these images with respect true geometry parenchyma, we analyzed IVM data alveoli mouse model comparison simultaneously acquired optical...

Optical coherence tomography (OCT), as a non-invasive technique for studying tissue morphology, is widely used in vivo studies, requiring high resolution and fast three-dimensional imaging. Based on light scattering it reveals micrometer sized substructures of the samples due to changes their optical properties therefore allows quantification specimen's geometry. Utilizing fluorescence microscopy further information can be obtained from molecular compositions embedded investigated object....

Recent studies in animal models provided proof-of-principle evidence for cell transplantation as a potential future therapeutic approach retinal pathologies humans such Retinitis pigmentosa or age-related macular degeneration. In this case, donor cells are injected into the eye order to protect replace degenerating photoreceptors pigment epithelium. However, currently there is no three-dimensional imaging technique available that allows tracking of migration and integration host tissue under...

In regenerative medicine, the zebrafish is a prominent animal model for studying degeneration and regeneration processes, e.g. of photoreceptor cells in retina. By means optical coherence tomography (OCT), these studies can be conducted over weeks using same individual hence reducing variability results. To allow an improvement retinal OCT imaging by suitable optics, we developed eye geometrical data obtained vivo dispersion encoded full range as well comprising gradient index (GRIN) lens...

Optical coherence tomography (OCT) is a technique, capable of high resolution and non-invasive 3D imaging in vivo by detection backscattered light from cellular sub structures. Due to visualization micrometer sized tissue constituents penetration depths up 2 mm, it already well established medical fields like ophthalmology dermatology. Laser scanning confocal microscopy (LSCM), on the contrary, gives further information structural components stained with suitable dyes. In combination, these...

Though artificial ventilation is an essential life-saving treatment, the mechanical behavior of lung tissue at alveolar level still unknown. Therefore, we need to understand response during this microscale in order develop new and more protective methods. Optical coherence tomography (OCT) combined with intravital microscopy (IVM) a promising tool for visualizing dynamics high spatial temporal resolution uninterruptedly ventilated rats. We present measurement setup using custom-made animal...

Este estudo objetivou demonstrar as injúrias morfológicas e fisiológicas provocadas pela poluição atmosférica por sílica em plantas correlacionar com a presença de níveis elevados na urina seres humanos área mineração rocha. Trata-se um transversal, observacional analítico que analisou espécie planta Piper gaudichaudianum 51 amostras pessoas dois pontos amostrais (poluído controle). Os atributos funcionais foliares foram comparados teste T registrados MEV para análise do material particulado...

A method for axial resolution improvement by adequate spectral data fusion of two parallel acquired disjunct wavelength bands in the 0.8 μm and 1.3 region field simultaneous dual-band optical coherence tomography (OCT) is presented. The applied domain OCT system illuminated a supercontinuum laser light source allows imaging at 800 nm 1250 with free-space resolutions better than 4.5 7 μm, respectively, over entire depth scan range. Each band analyzed an individual spectrometer A-scan rate 12...

Optical coherence tomography (OCT) is a noninvasive imaging modality generating cross sectional and volumetric images of translucent samples. In Fourier domain OCT (FD OCT), the depth profile calculated by fast transformation interference spectrum, providing speed SNR advantage thus making FD well suitable in biomedical applications. The spectrum can be acquired spectrally resolved spectral or time-resolved optical frequency (OFDI). Since still suffer from motion artifacts, especially under...

Acute lung injury (ALI) is a severe pulmonary disease leading to hypoxemia accompanied by reduced compliance and partial edema of the lung. Most patients have be ventilated compensate for lack oxygen. The treatment strongly connected with ventilator induced (VILI), which believed introduce further stress changes in its elastic performance. A thorough understanding organs micro-structure crucial gain more insight into course disease. Due backscattering near-infrared light, detailed...

For the study of disease mechanisms and development novel therapeutic strategies for retinal pathologies in human, rodent models play an important role. Nowadays, optical coherence tomography (OCT) allows three-dimensional investigation events over time. However, a detailed analysis how different degenerations are reflected OCT images is still lacking biomedical field. Therefore, we use to visualize degeneration specific mouse order progression <i>in vivo</i> improve image interpretation...

In critical care medicine, artificial ventilation is a life saving tool providing sufficient blood oxygenation to patients suffering from respiratory failure. Essential for their survival the use of protective strategies prevent further lung damage due ventilator induced injury (VILI). Since there only little known about implications tissue overdistension on alveolar level, especially in case diseased lungs, this research deals with investigation deformation microscale. A combined setup...

<h3>Background</h3> A characteristic feature of RA is the presence autoantibodies (AAB) such as rheumatoid factor (RF), anti-RA33, and antibodies to citrullinated peptides (ACPA) in a majority patients. RF ACPA are not only diagnostically helpful but also prognostic indicators for progression joint destruction. Over past years, health care research has increasingly focused on early diagnosis even prevention. Since disease significant morbidity, affecting app. 0,5 - 1% population leading...