A5071245024- Retinal Diseases and Treatments
- Retinal Imaging and Analysis
- Retinal Development and Disorders
- Glaucoma and retinal disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Single-cell and spatial transcriptomics
- Dental Implant Techniques and Outcomes
- Dental materials and restorations
- Zebrafish Biomedical Research Applications
- Retinal and Optic Conditions
- Optical Polarization and Ellipsometry
- Cerebral Venous Sinus Thrombosis
- Ocular Surface and Contact Lens
- Periodontal Regeneration and Treatments
- Corneal surgery and disorders
- Intraocular Surgery and Lenses
- Optical Imaging and Spectroscopy Techniques
- Optical Coherence Tomography Applications
- Acute Ischemic Stroke Management
- Retinal and Macular Surgery
- Ocular and Laser Science Research
- Connexins and lens biology
- Spectroscopy Techniques in Biomedical and Chemical Research
- Corneal Surgery and Treatments
- Ophthalmology and Visual Impairment Studies
Technische Universität Dresden
2010-2023
Center for Systems Biology Dresden
2023
Universitätsklinikum Aachen
2005-2014
Berlin-Brandenburger Centrum für Regenerative Therapien
2014
RWTH Aachen University
2003-2012
University Hospital Carl Gustav Carus
2010-2012
FH Aachen
2006-2008
Massachusetts Eye and Ear Infirmary
2004-2006
Smith-Kettlewell Eye Research Institute
2004-2006
Harvard University
2004-2006
ABSTRACT Zebrafish display widespread and pronounced adult neurogenesis, which is fundamental for their regeneration capability after central nervous system injury. However, the cellular identity biological properties of newborn neurons are elusive most brain areas. Here, we have used short-term lineage tracing radial glia progeny to prospectively isolate from her4.1+ in homeostatic forebrain. Transcriptome analysis glia, mature using single cell sequencing identified distinct...
In the lesioned zebrafish retina, Müller glia produce multipotent retinal progenitors that generate all neurons, replacing lost cell types. To study molecular mechanisms linking reactivity to progenitor production and neuronal differentiation, we used single-cell RNA sequencing of glia, regenerated progeny from uninjured light-lesioned retinae. We discover an injury-induced differentiation trajectory leads into a population with hybrid identity expressing marker genes progenitors. A glial...
Light-induced lesions are a powerful tool to study the amazing ability of photoreceptors regenerate in adult zebrafish retina. However, specificity lesion towards or regional differences within retina still incompletely understood. We therefore characterized process degeneration and regeneration an established paradigm, using intense white light from fluorescence lamp on swimming fish (diffuse lesion). also designed new paradigm where is focused through microscope onto immobilized (focused...
Fibroblast growth factors (Fgf) are secreted signaling molecules that have mitogenic, patterning, neurotrophic and angiogenic properties. Their importance during embryonic development in patterning morphogenesis of the vertebrate eye is well known, but less known about role Fgfs adult retina. To address Fgf function retina, we determined spatial distribution components pathway zebrafish We detected differential expression receptors, ligands downstream targets within specific retinal layers....
Inflammation can lead to persistent and irreversible loss of retinal neurons photoreceptors in mammalian vertebrates. In contrast, the adult zebrafish brain, acute neural inflammation is both necessary sufficient stimulate regeneration neurons. Here, we report on critical, positive role immune system support retina zebrafish. After sterile ablation by phototoxicity, find rapid response cells, especially monocytes/microglia neutrophils, which returns homeostatic levels within 14 days post...
Imaging polarimetry was used to examine different components of neovascular membranes in age-related macular degeneration. Retinal images were acquired with a scanning laser polarimeter. An innovative pseudocolor scale, based on cardinal directions color, displayed two types image information: relative phases and magnitudes birefringence. Membranes had phase changes that did not correspond anatomical structures reflectance images. Further, membrane borders depolarized light significantly...
In comparison to mammals, zebrafish are able regenerate many organs and tissues, including the central nervous system (CNS). Within CNS-derived neural retina, light lesions result in a loss of photoreceptors subsequent activation Müller glia, retinal stem cells. glia-derived progenitors differentiate eventually restore anatomical tissue architecture within 4 weeks. However, little is known about how impair vision functionally, as well what extent visual function restored during course...
We applied a polarimetric analysis to retinal imaging, examine the potential improvement in characterizing blood vessels. To minimize reflection artifact of superficial wall vessel, we computed depolarized light images by removing polarization retaining reaching instrument. These were compared from average all light. Michelson contrast was for vessel profiles across arteries and veins, higher images. Depolarized provide one step towards improving characterization
The evidence concerning the management of shortened dental arch (SDA) cases is sparse. This multi-center study was aimed at generating data on outcomes and survival rates for two common treatments, removable prostheses (RDP) molar replacement or no (SDA). hypothesis that treatments lead to different incidences tooth loss. We included 215 patients with complete loss in one jaw. Molars were either replaced by RDP not replaced, according SDA concept. First after treatment primary outcome...
The scientific evidence concerning prosthodontic care for the shortened dental arch (SDA) is sparse. This randomized multicenter study aimed to compare two common treatment options: removable partial prostheses (RPDPs) molar replacement vs. no (SDA). One of hypotheses was that follow-up differs between patients with RPDPs and SDAs during 5-year period. Two hundred fifteen complete loss in one jaw were included study. Molars either replaced by or not according SDA concept. A mean number 4.2...
To improve detection and quantification of peripapillary hyperpigmentation, associated with aging, open-angle glaucoma, age-related macular degeneration.A computational approach was implemented a readily available polarimeter used in glaucoma diagnosis, nerve fiber analyzer (GDx; Laser Diagnostic Technologies, San Diego, CA). Using near-infrared illumination at each 20 input polarizations, series image pairs digitized. One is made from the light returning eye that polarized parallel to...
Previous studies confirmed reduced retrobulbar haemodynamics in primary open-angle glaucoma (POAG).To investigate a correlation between and morphometric neuroretinal rim analysis patients with POAG.51 POAG (mean (standard deviation (SD)) age 65 (11) years) were included this clinical study. Blood flow velocities (peak systolic velocity (PSV) end-diastolic (EDV)) of the ophthalmic artery, central retinal artery (CRA), posterior ciliary arteries (PCA) vein measured using colour Doppler imaging...
The fovea is the retinal location responsible for our most acute vision. There are several methods used to localize fovea, but not always easily identifiable. Landmarks determine foveal variable in normal subjects and localization becomes even more difficult instances of disease. In subjects, photoreceptor axons that make up Henle fiber layer cylindrical radial orientation these fibers centered on fovea. exhibits form birefringence, which predictably changes polarized light scanning laser...
To investigate the impact of cataract surgery on association ocular pulse amplitude (OPA) and intraocular pressure (IOP) with respect to interpretation OPA as an estimate blood flow.Twenty-four patients were included in a clinical study. was measured using dynamic contour tonometry (DCT, Pascal(®), SMT Swiss Microtechnology AG, Switzerland). IOP by means Goldmann applanation (IOP GAT) DCT DCT). All measurements performed before one day after surgery.At baseline, correlated GAT (r = 0.67, P...