A5020843492- Microtubule and mitosis dynamics
- Gene Regulatory Network Analysis
- Fungal and yeast genetics research
- Genomics and Chromatin Dynamics
- Microbial Metabolic Engineering and Bioproduction
- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- DNA Repair Mechanisms
- Bioinformatics and Genomic Networks
- Protein Structure and Dynamics
- Plant nutrient uptake and metabolism
- Ubiquitin and proteasome pathways
- Epigenetics and DNA Methylation
- Photoreceptor and optogenetics research
- Cancer-related Molecular Pathways
- Chromosomal and Genetic Variations
- Bacterial Genetics and Biotechnology
- RNA Research and Splicing
- DNA and Nucleic Acid Chemistry
- Plant Reproductive Biology
- Reproductive Biology and Fertility
- Genetics, Bioinformatics, and Biomedical Research
- Light effects on plants
- Cellular transport and secretion
- Protist diversity and phylogeny
University of Oxford
2015-2024
Budapest University of Technology and Economics
2001-2013
Science Oxford
2013
Google (United States)
2011
Hungarian Academy of Sciences
2003-2007
The Honourable Society of Lincoln's Inn
2006-2007
Cancer Research UK
2006-2007
Budapest Institute
1986-2003
Collegium Budapest
2003
Virginia Tech
1993-2000
The adaptive responses of a living cell to internal and external signals are controlled by networks proteins whose interactions so complex that the functional integration network cannot be comprehended intuitive reasoning alone. Mathematical modeling, based on biochemical rate equations, provides rigorous reliable tool for unraveling complexities molecular regulatory networks. budding yeast cycle is challenging test case this approach, because control system known in exquisite detail its...
The molecular machinery of cell cycle control is known in more detail for budding yeast, Saccharomyces cerevisiae, than any other eukaryotic organism. In recent years, many elegant experiments on yeast have dissected the roles cyclin molecules (Cln1–3 and Clb1–6) coordinating events DNA synthesis, bud emergence, spindle formation, nuclear division, separation. These experimental clues suggest a mechanism principal interactions controlling synthesis degradation. Using standard techniques...
To contribute to a deeper understanding of M-phase control in eukaryotic cells, we have constructed model based on the biochemistry promoting factor (MPF) Xenopus oocyte extracts, where there is evidence for two positive feedback loops (MPF stimulates its own production by activating Cdc25 and inhibiting Wee1) negative loop destruction indirectly ubiquitin pathway that degrades cyclin subunit). uncover full dynamical possibilities system, translate regulatory network into set differential...
Abstract Following DNA damage caused by exogenous sources, such as ionizing radiation, the tumour suppressor p53 mediates cell cycle arrest via expression of CDK inhibitor, p21. However, role p21 in maintaining genomic stability absence DNA-damaging agents is unclear. Here, using live single-cell measurements protein proliferating cultures, we show that naturally occurring incurred over S-phase causes p53-dependent accumulation during mother G2- and daughter G1-phases. High levels mediate G1...
PP2A-B55 is one of the major phosphatases regulating cell division. Despite its importance for temporal control during mitotic exit, how B55 substrates are recognized and differentially dephosphorylated unclear. Using phosphoproteomics combined with kinetic modeling to extract B55-dependent rate constants, we have systematically identified assigned their order in exit. These share a bipartite polybasic recognition determinant (BPR) flanking Cdk1 phosphorylation site. Experiments show that...
p53 is activated in response to events compromising the genetic integrity of a cell. Recent data show that activity does not increase steadily with damage but rather fluctuates an oscillatory fashion (Lahav et al., Nature Genetics, 36, 147-150, 2004). Theoretical studies suggest oscillations can arise from combination positive and negative feedbacks or long feedback loop alone. Both are present p53/Mdm2 network, it known what roles they play DNA damage. We developed mathematical model based...
ABSTRACT An analysis was made of cell length and cycle time in time-lapse films the fission yeast Schizosaccharomyces pombe using wild-type (WT) cells those various mutants. The more important conclusions about ‘size controls’ are: (1) there is a marker G2 WT provided by rate change point (RCP) where linear growth increases ∼30%. period before this RCP dependent on size can be called ‘sizer’. after nearly independent ‘timer’. achievement critical threshold at or near which average 0.3...
Cytokinesis follows separase activation and chromosome segregation. This order is ensured in budding yeast by the mitotic exit network (MEN), where Cdc14p dephosphorylates key conserved Cdk1-substrates exemplified anaphase spindle-elongation protein Ase1p. However, metazoans, MEN Cdc14 function not conserved. Instead, PP2A-B55α/ENSA/Greatwall (BEG) pathway controls human Ase1p ortholog PRC1. In this pathway, PP2A-B55 inhibition coupled to Cdk1-cyclin B activity, whereas maintained cyclin...
The transition from G1 into DNA replication (S phase) is an emergent behavior resulting dynamic and complex interactions between cyclin-dependent kinases (Cdks), Cdk inhibitors (CKIs), the anaphase-promoting complex/cyclosome (APC/C). Understanding cellular decision to commit S phase requires a quantitative description of these interactions. We apply imaging single human cells track expression G1/S regulators use data parametrize stochastic mathematical model transition. show that rapid,...
The decision to replicate its DNA is of crucial importance for every cell and, in many organisms, decisive the progression through entire cycle. A comparison animals versus yeast has shown that, although most involved cell-cycle regulators are divergent both clades, they fulfill a similar role and overall network topology G1/S regulation highly conserved. Using germline development as model system, we identified regulatory cascade controlling entry into S phase flowering plant Arabidopsis...
The organisation of mammalian genomes into loops and topologically associating domains (TADs) contributes to chromatin structure, gene expression recombination. TADs many are formed by cohesin positioned CTCF. In proliferating cells, also mediates sister chromatid cohesion, which is essential for chromosome segregation. Current models folding cohesion based on assumptions how CTCF molecules organise the genome. Here we have measured absolute copy numbers dynamics cohesin, CTCF, NIPBL, WAPL...