A5079303125- Xenotransplantation and immune response
- Virus-based gene therapy research
- Animal Genetics and Reproduction
- Animal Virus Infections Studies
- Organ Transplantation Techniques and Outcomes
- Viral gastroenteritis research and epidemiology
- Cytomegalovirus and herpesvirus research
- Tissue Engineering and Regenerative Medicine
- Animal Disease Management and Epidemiology
- Chromosomal and Genetic Variations
- CRISPR and Genetic Engineering
- Pancreatic function and diabetes
- Hepatitis Viruses Studies and Epidemiology
- Viral Infectious Diseases and Gene Expression in Insects
- HIV Research and Treatment
- Transplantation: Methods and Outcomes
- Immunotherapy and Immune Responses
- Anesthesia and Neurotoxicity Research
- Viral Infections and Immunology Research
- Herpesvirus Infections and Treatments
- Pluripotent Stem Cells Research
- Hepatitis B Virus Studies
- Liver physiology and pathology
- Diabetes and associated disorders
- RNA Interference and Gene Delivery
Freie Universität Berlin
2020-2025
Robert Koch Institute
2014-2024
Ludwig-Maximilians-Universität München
2016-2024
Institute of Virology of the Slovak Academy of Sciences
2022
Institute of Immunology
2022
University of Padua
2022
Federal Institute for Risk Assessment
2022
Sydney Local Health District
2014
Paul Ehrlich Institut
1991-2003
Marie Curie
1990
Abstract Background Xenotransplantation using pig cells, tissues, or organs may be associated with the transmission of porcine microorganisms and development zoonoses. Among all endogenous retroviruses ( PERV s) represent a special risk because they are integrated in genome pigs able to infect human cells. In previous preclinical retrospective clinical trials xenotransplantation, no was observed. The first trial (alginate‐encapsulated) islet cell transplantation New Zealand, which approved...
Abstract The human endogenous retrovirus K family (HERV-K) comprises 30 to 50 closely related proviruses, most of which are defective. In contrast all other retroviruses, some HERV-K proviruses have maintained open reading frames for viral proteins. addition the structural proteins Gag and Env reverse transcriptase, two regulatory (Rec Np9) been described. Malignant melanoma has highest mortality among skin cancers is particularly aggressive. To study expression HERV-K, a set seven primers...
A novel gammaretrovirus named xenotropic murine leukemia virus-related virus (XMRV) has been recently identified and found to have a prevalence of 40% in prostate tumor samples from American patients carrying homozygous R462Q mutation the RNaseL gene. This impairs function innate antiviral type I interferon pathway is known susceptibility factor for cancer. Here, we attempt measure XMRV cancer cases Germany determine whether an analogous association with polymorphism exists.589 were...
Abstract: Background: Xenotransplantation using porcine cells, tissues or organs may be associated with the transmission of endogenous retroviruses (PERVs). More than 50 viral copies have been identified in pig genome and three different subtypes PERV were released from two them able to infect human cells vitro. RNA interference is a promising option inhibit transmission. Methods: We recently selected an efficient si (small interfering) corresponding highly conserved region DNA, which...
Abstract Xenotransplantation from pigs could alleviate the shortage of human tissues and organs for transplantation. Means have been identified to overcome hyperacute rejection acute vascular mechanisms mounted by recipient. The challenge is combine multiple genetic modifications enable normal animal breeding meet demand transplants. We used two methods colocate xenoprotective transgenes at one locus, sequential targeted transgene placement - ‘gene stacking’ cointegration engineered large...
Abstract The International Xenotransplantation Association has updated its original “Consensus Statement on Conditions for Undertaking Clinical Trials of Porcine Islet Products in Type 1 Diabetes,” which was published 2009. This update is timely and important light scientific progress changes the regulatory framework pertinent to islet xenotransplantation. Except chapter “informed consent,” remained relevant 2009 version, all other chapters included initial consensus statement have been...
Abstract Xenotransplantation using pig organs has achieved survival times up to 195 days in orthotopic heart transplantation into baboons. Here we demonstrate that addition an improved immunosuppressive regimen, non-ischaemic preservation with continuous perfusion and control of post-transplantation growth the transplant, prevention transmission porcine cytomegalovirus (PCMV) plays important role achieving long times. For first time PCMV xenotransplantation was associated a reduced...
Abstract: The objective of this study was to evaluate the feasibility and safety a hybrid liver support system with extracorporeal plasma separation bioreactor perfusion in patients acute failure (ALF) who had already fulfilled criteria for high urgency transplantation (LTx). Eight (one male, seven female) were treated terms bridging transplantation. mean age 36.5 yr (range 20 58). Etiology drug‐related two patients, hepatitis B infection three unknown patients. bioreactors charged primary...
The koala retrovirus (KoRV) is a gammaretrovirus closely related to the gibbon ape leukemia virus and induces leukemias immune deficiencies associated with opportunistic infections, such as chlamydiosis. Here we characterize KoRV newly isolated from an animal in German zoo show infection of human rat cell lines vitro rats vivo, using immunological PCR methods for detection. transmembrane envelope protein (p15E) was cloned expressed, p15E-specific neutralizing antibodies able prevent were...
The human endogenous retrovirus-K encodes two potential tumor proteins, Rec and Np9. is related to the Rev protein of HIV-1 has been shown be associated with development in nude mice. Having expression melanomas melanoma cell lines, tools were developed allow transmembrane envelope, Np9 mRNA proteins studied more detail. spliced env, rec np9 was investigated by reverse transcriptase-polymerase chain reaction using a set primers discriminate between full-length mRNA. Env-specific,...
Abstract The overall prognosis of patients with advanced melanoma is poor due to the lack effective treatment. A key factor for successful therapy an early detection disease. Therefore, reliably methods and meaningful tumor markers are required. Expression human endogenous retrovirus (HERV)‐K(HML‐2) was found elevated in melanomas it shown that HERV‐K supports vitro transition cells from adherent a more malignant, nonadherent phenotype. Furthermore, HERV‐K‐specific antibodies correlate...
Numerous copies of endogenous retroviruses are present in the genome mammals including man. Although most them defective, some, e.g., human HERV-K, were found to be expressed under certain physiological conditions. For instance, HERV-K is germ cell tumours and melanomas as well placenta. Most exogenous immunodeficiency virus HIV-1 induce severe immunodeficiencies there increasing evidence that transmembrane envelope (TM) proteins these may involved. We show here particles released from a...
Abstract: Xenotransplantation using porcine cells, tissues, or organs may offer a potential solution for the shortage of allogeneic human organs. Prior to clinical use xenotransplants, three main hurdles must be overcome: immunologic rejection, physiologic incompatibility, and risk transmission pathogens. Designated pathogen‐free breeding pigs can prevent most microbes. However, this is not possible in case endogenous retroviruses (PERV), which are integrated pig genome infect cells vitro....
Porcine circovirus 3 is a newly described circulating worldwide. PCV3 may play an etiologic role in different pig diseases. Two genotypes of were described, PCV3a and PCV3b. In order to analyse whether also present wild boars, animals living near Berlin studied. The had been analysed previously found form two genetically distinct geographically coherent clusters. To detect PCR was performed, the virus detail, parts sequence capsid protein sequenced. addition, screening for PCV1 PCV2...