A5005722236- Xenotransplantation and immune response
- Virus-based gene therapy research
- Animal Genetics and Reproduction
- Antimicrobial Resistance in Staphylococcus
- CRISPR and Genetic Engineering
- Organ Transplantation Techniques and Outcomes
- Diabetes and associated disorders
- Pancreatic function and diabetes
- Tissue Engineering and Regenerative Medicine
- Medical and Health Sciences Research
- Streptococcal Infections and Treatments
- Viral Infectious Diseases and Gene Expression in Insects
- Cardiac Ischemia and Reperfusion
- Biomedical Ethics and Regulation
- Neonatal and Maternal Infections
- Electrospun Nanofibers in Biomedical Applications
- Polish Historical and Cultural Studies
- Diabetes Management and Research
- Immunodeficiency and Autoimmune Disorders
- Transplantation: Methods and Outcomes
- Cytomegalovirus and herpesvirus research
- Infections and bacterial resistance
- Health and Medical Studies
- Law and Political Science
- Viral Infections and Immunology Research
Paul Ehrlich Institut
2008-2025
Hudson Institute
2023
The University of Sydney
2023
UCLouvain
2023
University of Fribourg
2023
Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital
2023
Klinikum Brandenburg
2008
Helmholtz Centre for Infection Research
2007
German Center for Infection Research
2004
Porcine cytomegalovirus (PCMV) is widely distributed in pigs and difficult to detect due latency. PCMV infection of source was associated with early graft failure after cardiac renal xenotransplantation into nonhuman primates. Importantly, the first genetically modified pig heart a human may have contributed reduced survival patient. Sensitive reliable assays for detection latent are thus indispensable. Here, we report development five peptide-induced rabbit antisera specific glycoprotein B...
Cardiac xenotransplantation has seen remarkable success in recent years and is emerging as the most promising alternative to human cardiac allotransplantation. Despite these achievements, acute vascular rejection still presents a challenge for long-term xenograft acceptance new insights into innate adaptive immune responses well detailed characterizations of signaling pathways are necessary. In allotransplantation, endothelial cells their sugar-rich surface—the glycocalyx—are known influence...
The blockade of the CD40/CD40L immune checkpoint is considered essential for cardiac xenotransplantation. However, it still unclear which single antibody directed against CD40 or CD40L (CD154), combination antibodies, better at preventing organ rejection. For example, high doses administered in previous experiments might not be feasible treatment humans, while thrombotic side effects were described first-generation anti-CD40L antibodies. To address these issues, we conducted six orthotopic...
Inflammatory responses and coagulation disorders are a relevant challenge for successful cardiac xenotransplantation on its way to the clinic. To cope with this, an effective clinically practicable anti-inflammatory anti-coagulatory regimen is needed. The inflammatory coagulatory response can be reduced by genetic engineering of organ-source pigs. Furthermore, there several therapeutic strategies prevent or reduce following xenotransplantation. However, it still unclear, which combination...
One of the major problems associated with development a vaccine against Streptococcus pyogenes is ability this pathogen to escape recognition by antibodies directed conserved surface-associated determinants and establish infection in setting an acquired immune response. Identification mechanism(s) used S. avoid antigen-specific killing blood was focus study. We showed here that capable surviving human containing high levels G-related α2-macroglobulin-binding protein GRAB, highly bacterial...
Abstract The infection of human transplant recipients by porcine endogenous retrovirus (PERV) is a safety issue for xenotransplantation (XTx). CRISPR/Cas9 technology has enabled the generation pigs free functional PERVs, and susceptibility these animals to reinfection PERVs remains unclear. To assess virological safety, we characterized cell line in which have been inactivated (PK15 clone 15 ) its infectious PERV. First, basal expression PERV pol , PERV‐A receptor (POPAR), reverse...
Abstract Background Porcine induced pluripotent stem cells (pi PSC s) offer an alternative strategy in xenotransplantation ( XT x). As human endogenous retroviruses HERV ), particularly ‐K, are highly expressed natural cells, we compared the expression of porcine PERV ) and retrotransposon LINE ‐1 (L1) open reading frames 1 2 pORF 2) different pi ‐like cell lines with their progenitors (porcine fetal fibroblasts, pFF ). Methods Cells reprogrammed via Sleeping Beauty‐transposed transcription...
Abstract Background Decellularized human pulmonary heart valve (dhHV) scaffolds have been shown to be the gold standard especially for younger, adolescent patients. However, valves are limited in availability. Xenogeneic decellularized pig (dpHV) may serve as alternative. Methods The efficacy of DNA reduction processes upon decellularization from German Landrace pigs was analyzed by measurements remaining nucleic acids including proviral porcine endogenous retrovirus (PERV) sequences....
GRAB (Protein G-related α2M-binding protein) is a surface protein of group A streptococci and exhibits high affinity for α2-macroglobulin (α2M), broad-range protease inhibitor. It the sole that has been shown to promote bacterial virulence in mouse model skin infection. The binding site α2M was predicted be N-terminal domain GRAB. In present study, first narrowed down 34 amino acids (amino 34–67) using variable truncated fusion proteins. sequence identified used design overlapping synthetic...
Xenotransplantation may compensate the limited number of human allografts for transplantation using pigs as organ donors. Porcine endogenous retroviruses inherit infectious potential if pig cells, tissues, or organs were transplanted to immunosuppressed recipients. Particularly, ecotropic PERV-C that could recombine with PERV-A highly replication-competent human-tropic PERV-A/C should be excluded from breeds designed xenotransplantation. Because their low proviral background, SLAD/D (SLA,...
Introduction: Porcine cytomegalovirus (PCMV) is widely distributed in pigs and difficult to detect due latency. PCMV infection of source was associated with early graft failure after cardiac renal xenotransplantation into nonhuman primates. Importantly, the first genetically modified pig heart a human may have contributed reduced survival patient. Sensitive reliable assays for detection latent are thus indispensable. Method: Here, we report development five peptide-induced rabbit antisera...
Using pigs as organ donors has advanced xenotransplantation to the point that it is almost ready for clinical use. However, there still a zoonotic risk associated with xenotransplantation, and potential transmission of porcine endogenous retroviruses needs be surveyed. Despite significant attempts eliminate this risk, by selection PERV-C free low expression PERV-A, -B, genome-wide inactivation PERV using CRISPR/Cas9, impact superinfection resistance (SIR) was not investigated. SIR viral...
Am 1. August 2007 trat in Deutschland das Gewebegesetz Kraft, welches unter anderem Regelungen hinsichtlich der Qualität und Sicherheit von Gewebezubereitungen (GWZ) trifft. Klassische GWZ sind Arzneimittel für die Anwendung am Menschen, a) menschliche Gewebe Zellen enthalten oder ausgehend diesen hergestellt werden, b) deren wesentliche Be- Verarbeitungsverfahren EU hinreichend bekannt bzw. neu, aber mit bekannten Verfahren vergleichbar sind, c) Wirkungen Nebenwirkungen aus dem...
Abstract Introduction Porcine endogenous retroviruses (PERVs) are an integral part of the pig genome with infectious potential, as shown in vitro. Hypothesis/gap statement In view nonclinical and clinical xenotransplantation, data essential that give insight into viral pathogenicity. This includes PERV's environmental stability risk. Aim We analyzed two ecotropic PERV‐C (PERV‐C[1312] ‐[5683]), monitoring cell‐free culture supernatants infected ST‐IOWA cells at various time intervals room...
Pig to human xenotransplantation represents an ambitious venture that requires, besides evasion of rejection mechanisms and physiological incompatibilities, the generation pathogen‐free pigs as donors for well characterized xenografts warrant medicinal products do comply with statutory regulatory demands [1–3]. The publication a high quality draft sequence pig genome (Sus scrofa) series accompanying reports first time offered feasibility whole expression profiling porcine tissues cells...
Introduction: Yucatan SLAD/D (SLA, swine leukocyte antigen) haplotype inbred miniature are candidates as organ donors for xenotransplantation. Porcine endogenous retroviruses (PERV) inherit infectious potential if pig cells, tissues, or organs were transplanted to immunosuppressed human recipients. Particularly, ecotropic PERV-C that could recombine with PERV-A highly replication-competent human-tropic PERV-A/C should be excluded from breeds designed pigs do not bear and –B, even they carry...
Introduction: Being on the verge of clinical application xenotransplantation (XTx) using pigs as organ donors experienced a tremendous progress. However, XTx still bears zoonotic risk which potential transmission porcine endogenous retroviruses (PERV) needs to be surveyed. Despite great efforts eliminate this e.g. by selection PERV-C free with low expression possibly functional PERV-A/-B and genome-wide inactivation PERV CRISPR/Cas9, effect superinfection resistance (SIR) was not...