A5064868554- Organ Transplantation Techniques and Outcomes
- Liver Disease and Transplantation
- Liver physiology and pathology
- Tissue Engineering and Regenerative Medicine
- Liver Disease Diagnosis and Treatment
- Pancreatic and Hepatic Oncology Research
- Renal Transplantation Outcomes and Treatments
- Electrospun Nanofibers in Biomedical Applications
- Pancreatic function and diabetes
- 3D Printing in Biomedical Research
- Hepatocellular Carcinoma Treatment and Prognosis
- Organ Donation and Transplantation
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Surgical Simulation and Training
- Clinical Nutrition and Gastroenterology
- Transplantation: Methods and Outcomes
- Colorectal Cancer Treatments and Studies
- Anatomy and Medical Technology
- MicroRNA in disease regulation
- Gastric Cancer Management and Outcomes
- Xenotransplantation and immune response
- Organ and Tissue Transplantation Research
- Esophageal Cancer Research and Treatment
- Esophageal and GI Pathology
- Augmented Reality Applications
Charité - Universitätsmedizin Berlin
2016-2025
Freie Universität Berlin
2017-2025
Humboldt-Universität zu Berlin
2004-2025
Berlin Institute of Health at Charité - Universitätsmedizin Berlin
2017-2024
Deutsche Forschungsgemeinschaft
2020-2024
Cluster of Excellence "Matters of Activity. Image Space Material"
2020-2024
Max Perutz Labs
2006
Triangle
2006
BOKU University
2006
University of Veterinary Medicine Vienna
2006
Background: While 4 randomized controlled clinical trials confirmed the early benefits of hypothermic oxygenated machine perfusion (HOPE), high-level evidence regarding long-term outcomes is lacking. The aim this follow-up study from HOPE-ECD-DBD trial was to compare in patients who underwent liver transplantation using extended criteria donor allografts donation after brain death (ECD-DBD), either HOPE or static cold storage (SCS). Methods: Between September 2017 and 2020, recipients...
Polyomaviruses are small, non-enveloped viruses with a circular double-stranded DNA genome. Using generic polyomavirus PCR targeting the VP1 major structural protein gene, novel was initially identified in resected human liver tissue and provisionally named Human Polyomavirus 12 (HPyV12). Its 5033 bp genome is predicted to encode large small T antigens 3 proteins VP1, VP2 VP3. Phylogenetic analyses did not reveal close relationship any known or animal polyomavirus. Investigation of organs,...
The paper evaluates the application of a mixed reality (MR) headmounted display (HMD) for visualization anatomical structures in complex visceral-surgical interventions. A workflow was developed and technical feasibility evaluated.Medical images are still not seamlessly integrated into surgical interventions and, thus, remain separated from procedure.Surgeons need to cognitively relate 2-dimensional sectional 3-dimensional (3D) during actual intervention. MR applications simulate 3D reduce...
Abstract: The objective of this study was to evaluate the feasibility and safety a hybrid liver support system with extracorporeal plasma separation bioreactor perfusion in patients acute failure (ALF) who had already fulfilled criteria for high urgency transplantation (LTx). Eight (one male, seven female) were treated terms bridging transplantation. mean age 36.5 yr (range 20 58). Etiology drug‐related two patients, hepatitis B infection three unknown patients. bioreactors charged primary...
The detoxification capacities of single-pass albumin dialysis (SPAD), the molecular adsorbents recirculation system, (MARS) and continuous veno-venous hemodiafiltration (CVVHDF) were compared in vitro . In each experiment 4,100 mL toxin-loaded human plasma was processed for 6.5 hours. MARS treatment (n = 6) undertaken combination with CVVHDF. For SPAD CVVHDF a high-flux hollow fiber hemodiafilter (identical to filter) used. Levels ammonia, urea, creatinine, bilirubin, bile acids determined....
Right lobe living donor liver transplantation (LD-LTx) is currently performed at an increasing number of transplant centers. Donor selection, safety, recovery, and postdonation psychological impairment are essential criteria to determine whether under which conditions LD-LTx justifiable. Before commencing the program, approval was obtained from local ethics committee. Potential donors underwent a comprehensive multistep evaluation protocol exclude any that could lead increased operative...
Objective Adipose tissue-derived factors link non-alcoholic fatty liver disease (NAFLD) with obesity, which has also been reported for circulating chemerin. On the other hand, hepatic chemerin and chemokine-like receptor 1 ( CMKLR1 ) mRNA expression not yet studied in an extensively characterized patient collective. Design This study was cross-sectional experimental design. Methods Liver tissue samples were harvested from 47 subjects histologically examined according to NAFLD activity score...
Abstract All memory T cells mount an accelerated response on antigen reencounter, but significant functional heterogeneity is present within the respective T-cell subsets as defined by CCR7 and CD45RA expression, thereby warranting further stratification. Here we show that several surface markers, including KLRB1, KLRG1, GPR56, KLRF1, help define low, high, or exhausted cytokine producers human peripheral intrahepatic CD4 + populations. Highest simultaneous production of TNF IFN-γ observed...
Decellularization of pancreata and repopulation these non-immunogenic matrices with islets endothelial cells could provide transplantable, endocrine Neo- Pancreata. In this study, rat were perfusion decellularized repopulated intact islets, comparing three routes (Artery, Portal Vein, Pancreatic Duct). effectively removed all cellular components but conserved the pancreas specific extracellular matrix. Digital subtraction angiography showed a integrity vascular system contrast emersion into...
Decellularization and recellularization of parenchymal organs may facilitate the generation autologous functional liver organoids by repopulation decellularized porcine matrices with induced cells. We present an accelerated (7 h overall perfusion time) effective protocol for human-scale decellularization pressure-controlled 1% Triton X-100 sodium dodecyl sulfate via hepatic artery (120 mmHg) portal vein (60 mmHg). In addition, we analyzed effect oscillating pressure conditions on pig (n=19)....
Reduced expression of the Indy (“I am Not Dead, Yet”) gene in lower organisms promotes longevity a manner akin to caloric restriction. Deletion mammalian homolog ( mIndy, Slc13a5 ) encoding for plasma membrane–associated citrate transporter expressed highly liver, protects mice from high‐fat diet–induced and aging‐induced obesity hepatic fat accumulation through mechanism resembling We studied possible role mIndy human metabolism. In obese, insulin‐resistant patients with nonalcoholic fatty...
Cell-based extracorporeal liver support is an option to assist or replace the failing organ until regeneration transplantation can be performed. The use of porcine cells tumor cell lines controversial. Primary human cells, obtained from explanted organs found unsuitable for transplantation, are a desirable source as they perform metabolism and regulation. Modular Extracorporeal Liver Support (MELS) concept combines different therapy units, tailored suit individual intra-individual clinical...
Introduction. The development of a bioreactor providing three-dimensional network interwoven capillary membranes with integrated oxygenation and decentralized mass exchange enables the culture primary human liver cells from discarded donor organs for extracorporeal support. Methods. Primary were isolated 54 (donor age 56.7±13.2 years). Between 2.8×1010 6.4×1010 parenchymal (PC) cocultured nonparenchymal (NPC) same organ in bioreactors (n=36). metabolic activity was regularly determined...
Problems with the limited availability of human hepatocytes for cell transplantation may be overcome by efficient cryopreservation techniques and formation appropriate banking. In this study we investigated effect disaccharide trehalose on hepatocytes. For analysis, liver cells were frozen in culture medium containing 10% dimethyl sulfoxide (DMSO) that was supplemented varying concentrations trehalose. During postthawing period, viability, plating efficiency, total protein, proliferation,...
Abstract: Demands for primary human hepatocytes are continuously increasing, while supply is insufficient due to limited cell sources. To improve availability, the present study investigates influence of donor liver characteristics on outcome hepatocyte isolation from surgically removed tissue ( n = 50). Hepatocytes were isolated specimens using a standardized two‐step collagenase perfusion technique. The patient's sex, previous chemotherapy, or histopathology have shown no influence. Donor...