A5085127273- Immune Cell Function and Interaction
- Immune Response and Inflammation
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Cancer-related gene regulation
- RNA modifications and cancer
- Immune cells in cancer
- T-cell and Retrovirus Studies
- Immunodeficiency and Autoimmune Disorders
- Glycosylation and Glycoproteins Research
- Cytokine Signaling Pathways and Interactions
- Protein purification and stability
- Cancer Research and Treatments
- RNA Research and Splicing
- Cancer Cells and Metastasis
- Psoriasis: Treatment and Pathogenesis
- Periodontal Regeneration and Treatments
- CAR-T cell therapy research
- Inflammasome and immune disorders
- Herpesvirus Infections and Treatments
- Tuberculosis Research and Epidemiology
- Biosimilars and Bioanalytical Methods
- Advanced Biosensing Techniques and Applications
- IL-33, ST2, and ILC Pathways
Charité - Universitätsmedizin Berlin
2005-2024
Berlin Institute of Health at Charité - Universitätsmedizin Berlin
2017-2023
Humboldt-Universität zu Berlin
1994-2019
Freie Universität Berlin
2017-2019
Berlin-Brandenburger Centrum für Regenerative Therapien
2017-2019
Research Institute Bioactive Polymer Systems
2010
Washington University in St. Louis
2010
Feinstein Institute for Medical Research
2010
MRC Laboratory of Molecular Biology
1995-2004
German Rheumatism Research Centre
2003
BCR-ABL is a chimeric oncogene generated by translocation of sequences from the c-abl protein-tyrosine kinase gene on chromosome 9 into BCR 22. Alternative proteins, p210BCR-ABL and p190BCR-ABL, are produced that characteristic chronic myelogenous leukemia acute lymphoblastic leukemia, respectively. Their role in etiology human remains to be defined. Transformed murine hematopoietic cells can used as model function since these made growth factor independent tumorigenic action oncogene. We...
The RBTN2 LIM-domain protein, originally identified as an oncogenic protein in human T-cell leukemia, is essential for erythropoiesis. A possible role transcription during erythropoiesis has been investigated. Direct interaction of the was observed vivo and vitro with GATA1 or -2 zinc-finger factors, well basic helix-loop-helix TAL1. By using mammalian two-hybrid analysis, complexes involving RBTN2, TAL1, GATA1, together E47, heterodimerization partner could be demonstrated. Thus, a...
BKV reactivation plays the causative role in development of BKV-associated nephropathy (BKVAN). Because lack effective therapy, early diagnosis is paramount for prevention BKVAN. Resting uroepithelial cells, excreted first urine before it can be detected plasma. The present study analyzed predictive value BK viruria viremia and its possible advantage BKVAN prediction.Total 4128 serum samples obtained from renal transplant patients were positivity by real-time polymerase chain reaction 433...
Abstract All memory T cells mount an accelerated response on antigen reencounter, but significant functional heterogeneity is present within the respective T-cell subsets as defined by CCR7 and CD45RA expression, thereby warranting further stratification. Here we show that several surface markers, including KLRB1, KLRG1, GPR56, KLRF1, help define low, high, or exhausted cytokine producers human peripheral intrahepatic CD4 + populations. Highest simultaneous production of TNF IFN-γ observed...
TREAT‐ME‐1, a Phase 1/2 open‐label multicenter, first‐in‐human, first‐in‐class trial, evaluated the safety, tolerability and efficacy of treatment with genetically modified autologous mesenchymal stromal cells (MSC), MSC_ apceth_101, in combination ganciclovir patients advanced gastrointestinal adenocarcinoma. Immunological inflammatory markers were also assessed. All (3 1; 7 2) received three cycles MSC_apceth_101 at one dose level on Day 0, 7, 14 followed by administration according to...
IL-10 is a potent immunoregulatory and anti-inflammatory cytokine. However, therapeutic trials in chronic inflammation have been largely disappointing. It well established that can inhibit Th1 Th2 cytokine production via indirect effects on APC. Less data are available about the influence of IL-17 production, which has recently linked to inflammation. Furthermore, there only few reports direct effect T cells. We demonstrate here directly interfere with TCR-induced IFN-gamma freshly isolated...
Platelet-rich plasma (PRP) therapy is increasingly popular to treat musculoskeletal diseases, including tendinopathies and osteoarthritis (OA). To date, it remains unclear which extent PRP compositions are determined by the immune cell cytokine profile of individuals or preparation method. investigate this, we compared leukocyte distributions different products donor blood samples assessed effect pro-inflammatory cytokines on chondrocytes.
Abstract Aims The gastrointestinal (GI) tract is composed of distinct sub-regions, which exhibit segment-specific differences in microbial colonization and (patho)physiological characteristics. Gut microbes can be collectively considered as an active endocrine organ. Microbes produce metabolites, taken up by the host actively communicate with immune cells gut lamina propria consequences for cardiovascular health. Variation bacterial load composition along GI may influence mucosal...
Abstract Interleukin‐10 (IL‐10), originally identified as an inhibitor of pro‐inflammatory cytokine production, exerts multiple immunomodulatory functions. Its ability to inhibit a Th1 response has been used in clinical trials for the treatment inflammatory diseases including psoriasis. However, little is known about molecular mechanisms IL‐10 We aimed at identifying possiblemediators vitro monocytes by gene chip technology using Hu95a Affymetrix mRNA arrays with 12,000 genes. To prove...
The LIM-only family of proteins comprises four members; two these (LMO1 and LMO2) are involved in human T-cell leukemia via chromosomal translocations, LMO2 is a master regulator hematopoiesis. We have carried out gene targeting the other members family, viz., genes Lmo1, Lmo3 Lmo4, to investigate their role mouse development. None has an obligatory lymphopoiesis. In addition, while null mutations Lmo1 or no discernible phenotype, mutation Lmo4 alone causes perinatal lethality due severe...
// Jobst C. von Einem 1 , Sylvia Peter 2 Christine Günther Hans-Dieter Volk 3 Gerald Grütz Christoph Salat 4 Oliver Stoetzer J. Nelson 5 Marlies Michl Dominik P. Modest Julian W. Holch Martin Angele 6 Christiane Bruns 7 Hanno Niess 6,* and Volker Heinemann 1,* Department of Medical Oncology Comprehensive Cancer Center, University Hospital Grosshadern, LMU, Munich, Germany Apceth GmbH & Co. KG, Institute for Immunology Berlin-Brandenburg Center Regenerative Medicine,...
Naive T helper (Th) lymphocytes are induced to express the il4 (interleukin-4) gene by simultaneous signaling through cell receptor and interleukin (IL)-4 receptor. Upon restimulation with antigen, such preactivated Th can reexpress independent of IL-4 signaling. This memory for expression depends on epigenetic modification locus an increased GATA-3, key transcription factor Th2 differentiation. Here, we have identified a phylogenetically conserved sequence, intronic regulatory element, in...
Abstract LPS hyporesponsiveness is characterized by a diminished production of proinflammatory cytokines which can be caused pretreatment with either (=LPS desensitization) or the combination anti-inflammatory IL-10 and TGF-β. However, resulting hyporesponsive states differ regarding their reversibility IFN-γ-inducing cytokine IL-12. Therefore, we aimed at studying reasons for this differential IL-12 responsiveness IFN-γ-producing cells its consequences in more detail. In an vitro...
Abstract Signal transducers and activators of transcription (STATs) are crucial molecules in cytokine signaling. In th conventional model STAT activation, recruited from a latent pool cytoplasmic monomers to the activated receptor. After binding receptor, they get tyrosine-phosphorylated, dissociate translocate nucleus as activation-induced dimers. Recently, several publications questioned this activation showed existence preassociated before activation. We were able demonstrate these STAT3...