A5058186432- Gut microbiota and health
- Diet and metabolism studies
- Dialysis and Renal Disease Management
- Sodium Intake and Health
- Alzheimer's disease research and treatments
- Probiotics and Fermented Foods
- Protein Structure and Dynamics
- Helicobacter pylori-related gastroenterology studies
- Clostridium difficile and Clostridium perfringens research
- Nutritional Studies and Diet
- Gastrointestinal motility and disorders
- Clinical Nutrition and Gastroenterology
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Blood Pressure and Hypertension Studies
- Enzyme Structure and Function
- IL-33, ST2, and ILC Pathways
- Digestive system and related health
- Protein Kinase Regulation and GTPase Signaling
- Protein Tyrosine Phosphatases
- NMR spectroscopy and applications
- Muscle metabolism and nutrition
- Gastroesophageal reflux and treatments
- Proteins in Food Systems
- Computational Drug Discovery Methods
- Eosinophilic Esophagitis
Max Delbrück Center
2020-2025
Charité - Universitätsmedizin Berlin
2020-2025
Humboldt-Universität zu Berlin
2021-2025
Freie Universität Berlin
2021-2025
German Centre for Cardiovascular Research
2024
European Molecular Biology Laboratory
2011
University of Leeds
2000-2004
University of Cambridge
2000
Dialysis-related amyloidosis (DRA) involves the aggregation of β2-microglobulin (β2m) into amyloid fibrils. Using Congo red and thioflavin-T binding, electron microscopy, X-ray fiber diffraction, we have determined conditions under which recombinant monomeric β2m spontaneously associates to form fibrils in vitro. Fibrillogenesis is critically dependent on pH ionic strength solution, with low high favoring fibril formation. The morphology formed varies growth conditions. At 4 0.4 M NaCl are...
CKD is characterized by a sustained proinflammatory response of the immune system, promoting hypertension and cardiovascular disease. The underlying mechanisms are incompletely understood but may be linked to gut dysbiosis. Dysbiosis has been described in adults with CKD; however, comorbidities limit CKD-specific conclusions.We analyzed fecal microbiome, metabolites, phenotypes 48 children (with normal kidney function, stage G3-G4, G5 treated hemodialysis [HD], or transplantation) mean±SD...
Dietary high salt (HS) is a leading risk factor for mortality and morbidity. Serum sodium transiently increases postprandially but can also accumulate at sites of inflammation affecting differentiation function innate adaptive immune cells. Here, we focus on how changes in extracellular sodium, mimicking alterations the circulation tissues, affect early metabolic, transcriptional, functional adaption human murine mononuclear phagocytes.Using Seahorse technology, pulsed stable...
Phosphatase of regenerating liver 3 (PRL-3) is suggested as a biomarker and therapeutic target in several cancers. It has well-established causative role cancer metastasis. However, little known about its natural substrates, pathways, biological functions, only few protein substrates have been so far. To improve our understanding the substrate specificity molecular determinants PRL-3 activity, wild-type (WT) protein, two supposedly catalytically inactive mutants D72A C104S, reported...
Journal Article Accepted manuscript Gut dysbiosis contributes to TMAO accumulation in CKD Get access Johannes Holle, Holle Experimental and Clinical Research Center, a cooperation of Charité – Universitätsmedizin Berlin Max Delbrück Center for Molecular Medicine, Berlin, GermanyMax Medicine the Helmholtz Association, GermanyDZHK (German Centre Cardiovascular Research), partner site GermanyDepartment Pediatric Gastroenterology, Nephrology Metabolic Diseases, - Germany Correspondence to:...
Abstract Aims The gastrointestinal (GI) tract is composed of distinct sub-regions, which exhibit segment-specific differences in microbial colonization and (patho)physiological characteristics. Gut microbes can be collectively considered as an active endocrine organ. Microbes produce metabolites, taken up by the host actively communicate with immune cells gut lamina propria consequences for cardiovascular health. Variation bacterial load composition along GI may influence mucosal...
Kidney transplantation (KT) is the best treatment for end-stage kidney disease, with graft survival critically affected by recipient's immune response. The role of gut microbiome in modulating this response remains underexplored. Our study investigates how alterations might associate allograft rejection analyzing using 16S rRNA gene amplicon sequencing a multicenter prospective involving 562 samples from 245 individuals which 217 received KT. Overall, composition showed gradual recovery...
Understanding the dynamic relationship between mucus-associated microbiota and host health is critical. However, studies predominantly using stool samples may not accurately represent these bacterial communities. Here, we investigated in gastrointestinal tract of mice terminal ileum humans different sample types: mucosal washes, brushes, scrapings, intestinal contents biopsies, brushes washes humans. We used DNA quantification 16S rRNA amplicon sequencing to evaluate comparability...
The intestine exhibits distinct characteristics along its length, with a substantial immune cell reservoir and diverse microbiota crucial for maintaining health. This study investigates how anatomical location regional influence intestinal abundance. Using conventionally colonized germ-free mice, segment-specific composition microbial communities were assessed. Metagenomic sequencing analyzed microbiome variations, while flow cytometry immunofluorescence examined composition. Microbiome...
Aims: The gastrointestinal (GI) tract is composed of distinct subregions which exhibit segment-specific differences in microbial colonization and (patho)physiological characteristics. Gut microbes can be collectively considered as an active endocrine organ. Microbes produce metabolites, taken up by the host actively communicate with immune cells gut lamina propria consequences for cardiovascular health. Variation bacterial load composition along GI may influence mucosal microenvironment thus...
Abstract Exploring the dynamic relationship between mucus-associated microbiota and host health is pivotal, yet prevalent studies using stool samples may not accurately represent these bacteria. Here, we explored in gastrointestinal tract of mice terminal ileum humans, three different sample types: mucosal washes, scraping, intestinal content biopsies washes humans. We employed DNA quantification 16S rRNA sequencing to assess how comparable information yielded from types, evaluating findings...
Abstract Background Kidney transplantation (KT) is the optimal treatment for end-stage kidney disease, with graft survival critically affected by recipient’s immune response. The role of gut microbiome in modulating this response remains underexplored. Our study investigates how alterations might associate allograft rejection. Methods We analyzed existing biomaterials a multicenter prospective involving 217 KT recipients and 28 donors from German Center Infection Research. Changes were using...
Abstract Background and Aims Chronic kidney disease (CKD) patients face a substantial morbidity risk, especially related to cardiovascular complications. The imbalance of circulating metabolites influences inter-organ crosstalk pathophysiology. In this regard, microbial origin have gained increasing attention. Trimethylamine N-oxide (TMAO) originates from (TMA) metabolism is found at high concentrations in the blood CKD patients. A TMAO concentration known be an independent risk factor for...
Abstract Chronic kidney disease (CKD) is characterized by a sustained pro-inflammatory response. The underlying mechanisms are incompletely understood, but may be linked to gut dysbiosis. Dysbiosis has been described in adults with CKD; however, comorbidities limit CKD-specific conclusions. We analyzed the fecal microbiome, metabolites and immune phenotypes children at three different CKD stages (G3-G4, G5 (hemodialysis), after transplantation) healthy controls. Serum TNF-α sCD14 were...
Introduction: Chronic inflammation is a major risk factor for cardiovascular disease in chronic kidney (CKD). The underlying mechanisms are incompletely understood, but may be linked to gut dysbiosis. We aim describe the microbiota-immune interaction cohort of pediatric CKD, thus independent confounding comorbidities frequently seen adult patients. Methods: analyzed fecal microbiome, plasma metabolites and peripheral immune phenotypes 48 children (normal function (HC, n=10), CKD stage G3-G4...
Conference Abstract| June 01 2000 From native state to amyloid; an investigation into partial unfolding of β-2-microglobulin Neil Kad; Kad *School Biochemistry and Molecular Biology, University Leeds, Leeds LS2 9JT, UK Search for other works by this author on: This Site PubMed Google Scholar Victoria McParland; McParland David Smith; Smith Anthony Brown; Brown #British Biotech Pharmaceuticals Ltd, Wallington road. Oxford, OX4 5LY. Mick Hunter; Hunter Sheena Radford Biochem Soc Trans (2000)...