A5102893380- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Protein Tyrosine Phosphatases
- Galectins and Cancer Biology
- Protein Kinase Regulation and GTPase Signaling
- Superconducting Materials and Applications
- Crystallography and molecular interactions
- PI3K/AKT/mTOR signaling in cancer
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Enzyme Structure and Function
- ATP Synthase and ATPases Research
- Melanoma and MAPK Pathways
- Biochemical and Molecular Research
- Plant Virus Research Studies
- Iron Metabolism and Disorders
- Microtubule and mitosis dynamics
- Particle accelerators and beam dynamics
- Spacecraft and Cryogenic Technologies
- T-cell and B-cell Immunology
- Migration, Health and Trauma
- Phytoplasmas and Hemiptera pathogens
- Genetics and Neurodevelopmental Disorders
- Chemotherapy-related skin toxicity
- Alkaline Phosphatase Research Studies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
University of Freiburg
2017-2025
European Molecular Biology Laboratory
2011-2021
Center for Research in Agricultural Genomics
2017
Institute of Agrifood Research and Technology
2017
Centro de Investigacion Principe Felipe
2005-2013
Migrant Clinicians Network
2001
Hospital Maciel
2000
General Electric (United States)
1977-1988
One is not enough: Two PD-1 pTyr motifs bind to SHP2 simultaneously, inducing a conformational change and phosphatase activation.
Fruit ripening is divided into climacteric and non-climacteric types depending on the presence or absence of a transient rise in respiration rate production autocatalytic ethylene. Melon ideal for study fruit ripening, as both varieties exist. Two introgressions accession PI 161375, encompassed QTLs ETHQB3.5 ETHQV6.3, 'Piel de Sapo' background are able to induce independently. We report that gene underlying ETHQV6.3 MELO3C016540 (CmNAC-NOR), encoding NAC (NAM, ATAF1,2, CUC2) transcription...
Phosphatase of regenerating liver 3 (PRL-3) is suggested as a biomarker and therapeutic target in several cancers. It has well-established causative role cancer metastasis. However, little known about its natural substrates, pathways, biological functions, only few protein substrates have been so far. To improve our understanding the substrate specificity molecular determinants PRL-3 activity, wild-type (WT) protein, two supposedly catalytically inactive mutants D72A C104S, reported...
In the melon exotic accession PI 161375, gene cmv1, confers recessive resistance to Cucumber mosaic virus (CMV) strains of subgroup II. cmv1 prevents systemic infection by restricting bundle sheath cells and impeding viral loading phloem. Here we report fine mapping cloning cmv1. Screening an F2 population reduced region a 132 Kb interval that includes Vacuolar Protein Sorting 41 gene. CmVPS41 is conserved among plants, animals yeast required for post-Golgi vesicle trafficking towards...
Abstract The receptor-linked protein tyrosine phosphatases (RPTPs) are key regulators of cell-cell communication through the control cellular phosphotyrosine levels. Most human RPTPs possess an extracellular receptor domain and tandem intracellular phosphatase domains: comprising active membrane proximal (D1) inactive distal (D2) pseudophosphatase domain. Here we demonstrate that PTPRU is unique amongst in possessing two domains. PTPRU-D1 displays no detectable catalytic activity against a...
Reproductive control of invasive, feral, and domesticated animals is an urgent public health issue. Immunological vaccines are employed as emerging strategy to target different components the reproductive system, achieve immunocastration. Successful use immunogens against parts oocyte, spermatozoon, gonadotropin-releasing hormone (GnRH), luteinizing receptor (LHr) has been reported. In this work, a recombinant DNA vaccine was prepared (P2GnRHrP30) by fusion first extracellular domain canine...
Src homology 2 (SH2) domain‐containing phosphatase (SHP2) mediates important signal transduction upon cell surface receptor stimulation, regulating multiple cellular functions. In addition to the catalytically active phosphotyrosine (pTyr) domain, SHP2 contains two regulatory pTyr‐binding domains: N‐SH2 and C‐SH2 domains. While role of domain is well understood, less clear. To support studies on involvement domains in function, here we describe development a peptide inhibitor containing...
Protein phosphatase-1 (PP1)-disrupting peptides (PDPs) are selective chemical modulators of PP1 that liberate the active catalytic subunit from regulatory proteins; thus allowing dephosphorylation nearby substrates. We have optimized original cell-active PDP3 for enhanced stability, and obtained insights into requirements stabilizing this 23-mer peptide cellular applications. The PDP-Nal was used to dissect involvement in MAPK signaling cascade. Specifically, we demonstrated that, human...
An Electric Power Research Institute sponsored study of superconducting, central-station turbine-generators 300 and 1200 MVA rating has been completed at General Electric. A unique construction concept is used for the superconducting field winding. The goal this to ensure that winding can be energized current flux density predicted by superconductor short-sample data, without "training" instabilities frequently encountered in large coils. remainder rotor components includes an aluminum...
Protein phosphatase‐1 ( PP 1) drives a large amount of phosphoSer/Thr protein dephosphorylations in eukaryotes to counteract multiple kinases signaling pathways. The phosphatase requires divalent metal cations for catalytic activity and contains iron naturally. Iron has been suggested have an influence on 1 through Fe 2+ 3+ oxidation states. However, much biochemical all structural data obtained with recombinant containing Mn ions. Purifying iron‐containing from Escherichia coli thus far not...
Natural polyphenols like oligomeric catechins (procyanidins) derived from green tea and herbal medicines are interesting compounds for pharmaceutical research due to their ability protect against carcinogenesis in animal models. It is nevertheless still unclear how intracellular pathways modulated by polyphenols. Monomeric were shown affect the activity of some protein phosphatases (PPs). The three regenerating liver (PRLs) close relatives promising therapeutic targets cancer. In present...
Vaccinia H1-related (VHR) phosphatase is a dual specificity that required for cell-cycle progression and plays role in cell growth of certain cancers. Therefore, it represents potential drug target. VHR structurally biochemically well characterized, yet its regulatory principles are still poorly understood. Understanding regulation important, not only to comprehend VHR's biological mechanisms roles but also determine druggability as target cancer. Here, we investigated the functional unique...
A 20 MVA superconducting generator has been designed and constructed. Major design issues are discussed in this paper, the chosen solutions described, reasons for their selection presented. component-by-component description of construction process is provided.
Abstract Increasing rates of HIV infection have been found in migrant farmworkers the USA over past decade. By virtue lifestyle, language and culture, these workers are not exposed to typical media prevention messages. To determine their level knowledge about this disease for use messages targeted specifically population, five gender specific focus groups were conducted among Haitian, Jamaican African-American upstate New York. The revealed that health belief system Afro-American primarily...
Mitogen-activated protein kinase (MAPK) pathways are well conserved in most organisms, from yeast to humans. The principal components of these MAP kinases whose activity is regulated by phosphorylation, implicating various MAPK effectors-in particular, phosphatases that inactivate MAPKs dephosphorylation. molecular basis binding specificity such regulatory poorly understood. To try pinpoint potential functional regions within the sequences and help identify new family members, we have...
The single‐copy mouse gene Ptprr gives rise to different protein tyrosine phosphatase (PTP) isoforms in neuronal cells through the use of distinct promoters, alternative splicing, and multiple translation initiation sites. Here, we examined array post‐translational modifications imposed on PTPRR PTPBR7, PTP‐SL, PTPPBSγ42 PTPPBSγ37, which have N‐terminal segments localize parts cell. All were found be short‐lived, constitutively phosphorylated proteins. In addition, transmembrane isoform, was...
Phosphatase of regenerating liver (PRL)-3 (PTP4A3) has gained much attention in cancer research due to its involvement tumor promoting and metastatic processes. It belongs the protein tyrosine phosphatase (PTP) superfamily is thought follow catalytic mechanism shared by this family, which aside from conserved active-site amino acids includes a glutamic acid residue that usually required for integrity active site PTPs. We noted structures PRL-3, PRL-1, PTEN these residues do not clearly align...