Abstract A single step approach to tailored nanoparticle‐bioconjugates, enabling the generation of gold nanoparticles by laser ablation and their in situ conjugation with any biomolecule bearing an electron donating function (e.g., thiolated oligonucleotides) is established. The integrity oligonucleotides after stability bioconjugates physiological media are investigated. Their size tailorable via process parameters. This rapid universal method may provide biochemists various...

Gold nanoparticles (GNPs) have interesting optical properties, such as exceptionally high quantum yields and virtually limitless photostability. Therefore, they show the potential for applications biomarkers especially suitable in vivo long-term studies. The generation of GNPs using pulsed laser light rather than chemical means provides nanoparticles, which are remarkably stable a variety media without need stabilizing agents or ligands. This stabilization is achieved by partial oxidation...

To improve well-known titanium implants, pores can be used for increasing bone formation and close bone-implant interface. Selective Laser Melting (SLM) enables the production of any geometry was implant with 250-µm pore size. The size supports vessel ingrowth, as is strongly dependent on fast vascularization. Additionally, proangiogenic factors promote functionalize factors, polycaprolactone (PCL) coating used. following were examined: vascular endothelial growth factor (VEGF), high...

To examine gold nanoparticle reprotoxicity, bovine spermatozoa were challenged with ligand-free or oligonucleotide-conjugated nanoparticles synthesized purely without any surfactants by laser ablation. Sperm motility declined at mass dose of 10 µg/ml (corresponding to ∼14 000 per sperm cell) regardless surface modification. morphology and viability remained unimpaired all concentrations. Transmission electron microscopy showed an modification dependant attachment the cell membrane...

Gold nanoparticles (AuNPs) are promising imaging agents for the long-term visualization and tracing of intracellular functions because they bear outstanding optical properties fairly easily bioconjugated. However, design such multifunctional nanosystems might be limited by their bioavailability. Cell-penetrating peptides (CPPs) have been shown to efficient molecular transporters with very few indices cytotoxicity also in conjunction nanoparticles. In this context, current work aims explore...

Summary In this study, the production of poly(ethylene glycol) diacrylate (PEGDA) scaffolds by micro‐stereolithography based on diode laser curing (DLC) is demonstrated. Sample are also loaded with acetylsalicylic acid (ASS) as model drug. The samples immersed in physiological NaCl solution and drug release characteristics tested. We show that DLC a useful technique to prepare drug‐loaded PEGDA scaffolds. compressive strength enriched DLC‐cured between 7 10 MPa, dependent ASS concentration....

Gebändigte Kraft: Ein photoaktivierbares cyclisches Peptid, das auf einer Oberfläche verankert ist, kann die zeitlich und örtlich definierte Anlagerung von Zellen bei Bestrahlung mit λ=351 nm bewirken. Das inaktive Peptid bindet nicht an Integrin, während aktivierte Form nach der effizient Zelladhäsion vermittelt, wie Mikroskopiebild belegt, durch eine Maske in 100 μm breiten Streifen belichtet wurde. Supporting information for this article is available on the WWW under...

Drug-coated balloons are medical devices designed to locally deliver drug diseased segments of the vessel wall. For these dosage forms, transfer wall needs be examined in detail, since released into blood is cleared from site. In order examine transfer, a new vitro setup was developed combining estimation loss during advancement site application model coronary artery pathway with hydrogel compartment representing, as very simplified model, The fluorescent substances well paclitaxel coated...

Drug-coated balloons (DCB), which have emerged as a therapeutic alternative to drug-eluting stents in percutaneous cardiovascular intervention, are well described with regard clinical efficacy and safety within number of studies. In vitro studies elucidating the correlation between coating additive DCB performance however rare but considered important for understanding requirements improvement established DCB. this regard, we examined three different DCB-systems, were developed former based...

Degradable implant material for bone remodeling that corresponds to the physiological stability of has still not been developed. Promising degradable materials with good mechanical properties are magnesium and alloys. However, excessive gas production due corrosion can lower biocompatibility. In present study we used polymer coating polycaprolactone (PCL), intended rate magnesium. Additionally, improvement geometry increase remodeling. Porous structures known support vessel ingrowth thus...

Contents The current demand for female calves has grown rapidly and controlling the sex of offspring provides an economically flexible management livestock producer. only functioning method efficiently producing separate populations X Y sperm in mammals is based on relative DNA differentiation by high‐speed flow cytometry. In this context, gold nanoparticles conjugated to chromosome‐specific moieties display promising application as novel fluorophor‐alternative high‐throughput screening,...

Abstract Aiming at a speed up of the re‐endothelialization process biodegradable endovascular implants, novel approaches for functionalization poly( l ‐lactide) (PLLA) with anti‐CD34 antibodies were established. We propose three‐step involving PLLA surface activation functional amino groups, attachment protein repelling peptide spacer, and covalent random or site‐selective immobilization antibodies. Obtainable antibody densities antigen binding capacities thoroughly evaluated by means...

Abstract Since late 1990s, polyetheretherketone (PEEK) has presented a promising polymeric alternative to metal implant components, particularly in orthopedic and traumatic applications. However, PEEK is biologically inert, which constrained its potential In this manner, enhancing the bioactivity of huge challenge that must be comprehended completely understand advantages. Up now, two noteworthy methodologies are discussed enhance PEEK, including bulk surface modification. Although latter...

Abstract. Hodgkin and Huxley were the pioneers to abstract biological neuron as an electric circuit nerve signal voltage impulse. The Hodgkin-Huxley theory (Hodgkin Huxley, 1952) has set direction defined goals for much of ensuing research in biophysics. However, 2005, T. Heimburg A. D. Jackson, biophysicists from Copenhagen proposed a new neural called Soliton (Heimburg 2005). In this theory, conduction is density wave. paper, theories are described theoretical comparison been carried out...

Beyond their originally sole mechanical function, current drug-eluting stents (DES) implement the concept of local drug delivery for re-opening stenotic arterial vessels, and prevention in-stent restenosis as one major limitations conventional bare metal (BMS). Current DES consist a permanent metallic stent platform an active agent being released from drug-incorporated polymer coating or porous surface. Although have impressively demonstrated capability reducing restenosis, safety remains...

Calcium alginate and polyacrylamide hydrogels were used as tissue models for the simulated anatomic implantation process.

Titanium is widely used as a bone implant material due to its biocompatibility and high resilience. Since Young’s modulus differs from tissue, the resulting “stress shielding” could lead scaffold loosening. However, by using scaffold-shaped geometry, can be adjusted. Also, porous geometry enables vascularisation ingrowth inside itself. Additionally, growth factors improve these effects. In order create deposit release system for factors, titanium scaffolds coated with degradable polymers....

The development of drug‐eluting coatings based on hyaluronic acid (HA) is especially promising for implant‐associated local drug delivery (LDD) systems, whose implantation provokes high insertion forces, as, instance, cochlear implants or drug‐coated balloons (DCB). lubricious character HA can then reduce the coefficient friction and serve as reservoir simultaneously. In this context, we investigated several plasma‐ wet‐chemical methods deposition HA‐based with LDD function polyamide 12 a...