A5059269914- Cellular Mechanics and Interactions
- Advanced Fluorescence Microscopy Techniques
- Lipid Membrane Structure and Behavior
- Microtubule and mitosis dynamics
- Micro and Nano Robotics
- Psychiatric care and mental health services
- Force Microscopy Techniques and Applications
- Alcoholism and Thiamine Deficiency
- Nuclear Structure and Function
- RNA Research and Splicing
- Advanced Electron Microscopy Techniques and Applications
- Health and Medical Studies
- Psychosomatic Disorders and Their Treatments
- Genetics, Aging, and Longevity in Model Organisms
- Biocrusts and Microbial Ecology
- 3D Printing in Biomedical Research
- Psychoanalysis and Social Critique
- Spaceflight effects on biology
- Immunotherapy and Immune Responses
- Molecular Junctions and Nanostructures
- T-cell and B-cell Immunology
- Meningioma and schwannoma management
- Monoclonal and Polyclonal Antibodies Research
- Erythrocyte Function and Pathophysiology
- Neurology and Historical Studies
Universität Innsbruck
2025
Institute of Photonic Sciences
2019-2024
Klinikum Klagenfurt
1995-2018
Innsbruck Medical University
2015-2016
Institute of Science and Technology Austria
2014-2016
Johannes Kepler University of Linz
2007-2011
Délégation Provence et Corse
2011
Centre d’Immunologie de Marseille-Luminy
2010-2011
Heidelberg University
2011
Institut de Neurobiologie de la Méditerranée
2011
3D amoeboid cell migration is central to many developmental and disease-related processes such as cancer metastasis. Here, we identify a unique prototypic mode in early zebrafish embryos, termed stable-bleb migration. Stable-bleb cells display an invariant polarized balloon-like shape with exceptional speed persistence. Progenitor can be reversibly transformed into irrespective of their primary fate motile characteristics by increasing myosin II activity through biochemical or mechanical...
The nucleus makes the rules Single cells continuously experience and react to mechanical challenges in three-dimensional tissues. Spatial constraints dense tissues, physical activity, injury all impose changes cell shape. How can measure shape deformations ensure correct tissue development homeostasis remains largely unknown (see Perspective by Shen Niethammer). Working independently, Venturini et al. Lomakin now show that act as an intracellular ruler cellular variations. nuclear envelope...
Nucleosomes form heterogeneous groups in vivo, named clutches. Clutches are smaller and less dense mouse embryonic stem cells (ESCs) compared to neural progenitor (NPCs). Using coarse-grained modeling of the pluripotency Pou5f1 gene, we show that genome-wide clutch differences between ESCs NPCs can be reproduced at a single gene locus. Larger formation is associated with changes compaction internucleosome contact probability fiber. single-molecule tracking (SMT), further core histone protein...
We present a structured illumination microscopy based point localization estimator (SIMPLE) that achieves 2-fold increase in single molecule precision compared to conventional centroid estimation methods. SIMPLE advances the recently introduced MINFLUX concept by using precisely phase-shifted sinusoidal wave patterns as nanometric rulers for simultaneous particle on photon count variation over 20 μm field of view. validate silico and experimentally TIRF-SIM setup digital micro-mirror device...
Through the asymmetric distribution of messenger RNAs (mRNAs), cells spatially regulate gene expression to create cytoplasmic domains with specialized functions. In neurons, mRNA localization is required for essential processes such as cell polarization, migration, and synaptic plasticity underlying long-term memory formation. The components driving transport in neurons mammalian are not known. We report first reconstitution a system revealing that tumor suppressor adenomatous polyposis coli...
The development of a polarized neuron relies on the selective transport proteins to axons and dendrites. Although it is well known that microtubule cytoskeleton has central role in establishing neuronal polarity, how its specific organization established maintained poorly understood. Using vivo model system Caenorhabditis elegans, we found highly conserved UNC-119 protein provides link between membrane-associated Ankyrin (UNC-44) microtubule-associated CRMP (UNC-33). Together they form...
Resolving the dynamical interplay of proteins and lipids in live-cell plasma membrane represents a central goal current cell biology. Superresolution concepts have introduced means capturing spatial heterogeneity at nanoscopic length scale. Similar for detecting transitions (superresolution chronoscopy) are still lacking. Here, we show that recently spot-variation fluorescence correlation spectroscopy allows sensing transient confinement times constituents dramatically improved resolution....
Nonadherent polarized cells have been observed to a pearlike, elongated shape. Using minimal model that describes the cell cortex as thin layer of contractile active gel, we show anisotropy stresses, controlled by cortical viscosity and filament ordering, can account for this morphology. The predicted shapes be determined from flow pattern only; they prove independent mechanism at origin flow, are only weakly sensitive cytoplasmic rheology. In case actin flows resulting instability, propose...
Abstract Super-resolution fluorescence microscopy has become a powerful tool in cell biology to observe sub-cellular organization and molecular details below the diffraction limit of light. methods are generally classified into three main concepts: stimulated emission depletion (STED), single molecule localization (SMLM) structured illumination (SIM). Here, we highlight novel concept modulation-enhanced (meLM) which designate as 4 th super-resolution method. Recently, series have emerged,...
Centrosomes play a crucial role during immune cell interactions and initiation of the response. In proliferating cells, centrosome numbers are tightly controlled generally limited to one in G1 two prior mitosis. Defects regulating have been associated with transformation tumorigenesis. Here, we report emergence extra centrosomes leukocytes activation. Upon antigen encounter, dendritic cells pass through incomplete mitosis arrest subsequent phase leading tetraploid accumulated centrosomes....
Biomechanical alterations contribute to the decreased regenerative capacity of hematopoietic stem cells (HSCs) upon aging. RhoA is a key regulator mechano-signaling but its role for mechanotransduction in cell aging has not been investigated yet. Here, we show that murine HSCs respond increased nuclear envelope (NE) tension by inducing NE translocation P-cPLA2, which intrinsically activates RhoA. Interestingly, aged experience physiologically higher intrinsic tension, associated with P-cPLA2...
<title>Abstract</title> Biomechanical alterations contribute to the decreased regenerative capacity of hematopoietic stem cells (HSCs) upon aging. RhoA is a key regulator mechano-signaling but its role for mechanotransduction in cell aging has not been investigated yet. Here, we show that murine HSCs respond increased nuclear envelope (NE) tension by inducing NE translocation P-cPLA2, which intrinsically activates RhoA. Interestingly, aged experience physiologically higher intrinsic tension,...
Abstract A growing body of work suggests that the material properties biomolecular condensates ensuing from liquid–liquid phase separation change with time. How this aging process is controlled and whether distinct can have different biological functions currently unknown. Using Caenorhabditis elegans as a model, we show MEC-2/stomatin undergoes rigidity transition fluid-like to solid-like facilitate transport mechanotransduction, respectively. This switch triggered by interaction between...
Abstract Spontaneous locomotion is a common feature of most metazoan cells, generally attributed to the fundamental properties actomyosin network. This force-producing machinery has been studied down minute molecular details, especially in lamellipodium-driven migration. Nevertheless, how networks work inside contraction-driven amoeboid cells still lacks unifying principles. Here, using stable motile blebs as model system, we image dynamics actin cortex at single filament level and reveal...
In the past decade carbon nanotubes (CNTs) have been widely studied as a potential drug-delivery system, especially with functionality for cellular targeting. Yet, little is known about actual process of docking to cell receptors and transport dynamics after internalization. Here we performed single-particle studies folic acid (FA) mediated CNT binding human carcinoma cells their inside cytosol. particular, employed molecular recognition force spectroscopy, an atomic microscopy based method,...